RAPD Analysis of Somaclonal Variants Derived from Embryo Callus Cultures of Peach
Abstract
Peach [Prunus persica (L.) Batsch] regenerants from cv 'Sunhigh' embryo no. 156, regenerants obtained from cv 'Redhaven' embryo no. 30, and two peach cultivars 'Sunhigh' and 'Redhaven', were screened for polymorphic RAPD (Random Amplified Polymorphic DNA) markers with up to 60 10-mer primers. Although 35 primers produced results with scoreable bands, only 10 of the primers revealed polymorphism for regenerants of embryo no. 156 and cv 'Sunhigh', and 1 revealed a low level of polymorphism for regenerants of embryo no. 30 and cv 'Redhaven'. This study demonstrates the feasibility of using RAPD markers to identify somaclonal variants of peach and provides evidence for the existence of genetic differences among these variants.
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RAPD can sometimes yield inconsistent results and may not be as reproducible as other methods such as AFLP and SSR.
“Despite its simplicity and low cost, RAPD can sometimes yield inconsistent results and may not be as reproducible as other methods, such as AFLP and SSR [121,122].”
From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [121]
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References 26 · Cited by 102
- Authors
- Hashmi, G., Huettel, R., Meyer, R., Krusberg, L., & Hammerschlag, F.
- Affiliations
- Agricultural Research Service (US)
- University of Maryland, College Park (US)
- ORCID
- Meyer, R.
- Venue
- Plant Cell Reports
- Published
- 1997-06
- Volume
- 16
- Issue
- 9
- Pages
- 624-627
- ISSN
- 0721-7714 (ISSN-L)
- 1432-203X
- OpenAlex
- W2052677050
- Cited by
- 102
- References
- 26
- Citations by year
- 2025: 1 · 2024: 3 · 2023: 2 · 2022: 2 · 2021: 1 · 2020: 2 · 2019: 2 · 2017: 2 · 2016: 3 · 2015: 1 · 2014: 4 · 2013: 3 · 2012: 7
- FWCI
- 1.06
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Plant Cell Reports (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Mapping and Diversity in Plants and Animals
- Keywords
- somaclonal variation, peach, RAPD markers, plant regeneration, genetic polymorphism, Redhaven cultivar, micropropagation
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