Cell Culture for Three-Dimensional Modeling in Rotating-wall Vessels: an Application of Simulated Microgravity
Abstract
High-density, three-dimensional cell cultures are difficult to grow in vitro. The rotating-wall vessel (RWV) described here has cultured BHK-21 cells to a density of 1.1 X 10(7) cells/ml. Cells on microcarriers were observed to grow with enhanced bridging in this batch culture system. The RWV is a horizontally rotated tissue culture vessel with silicon membrane oxygenation. This design results in a low-turbulence, low-shear cell culture environment with abundant oxygenation. The RWV has the potential to culture a wide variety of normal and neoplastic cells.
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Rotating wall vessel bioreactors utilize low shear, low turbulence biomechanical forces to influence cell differentiation and aggregation in three dimensions as a suspension.
“Rotating wall vessel bioreactors or rotary cell culture systems (Figure 2) utilize low shear, low turbulence biomechanical forces to influence cell differentiation and aggregation in three dimensions as a suspension (Schwarz et al., 1992).”
From Three-dimensional modelling of ovarian cancer: from cell lines to organoids for discovery and personalized medicine (2022) · cites this paper as Schwarz et al., 1992
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References 18 · Cited by 399
- Authors
- Schwarz, R. P., Goodwin, T. J., & Wolf, D. A.
- Affiliations
- Johnson Space Center (US)
- ORCID
- Goodwin, Thomas J.
- Venue
- Journal of Tissue Culture Methods
- Published
- 1992-06
- Volume
- 14
- Issue
- 2
- Pages
- 51-57
- ISSN
- 1381-5741 (ISSN-L)
- 0271-8057
- 1573-0603
- OpenAlex
- W2016138335
- Cited by
- 399
- References
- 18
- Citations by year
- 2026: 4 · 2025: 9 · 2024: 7 · 2023: 13 · 2022: 17 · 2021: 18 · 2020: 17 · 2019: 12 · 2018: 17 · 2017: 13 · 2016: 17 · 2015: 16 · 2014: 13 · 2013: 13 · 2012: 18
- FWCI
- 2.12
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Methods in Cell Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Spaceflight effects on biology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- rotating wall vessel, simulated microgravity, 3D cell culture, microcarriers, BHK-21 cells, high cell density culture, tumor cell culture
- MeSH terms
- Animals, Cell Count, Cell Line, Equipment Design, Cricetinae, Kidney, Microspheres, Rotation, Weightlessness Simulation, Cell Culture Techniques, Bioreactors
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