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Lipid hydroperoxide measurement by oxidation of Fe2+ in the presence of xylenol orange. Comparison with the TBA assay and an iodometric method
Abstract
Study of the role of hydroperoxides and lipid peroxidation in disease requires simple and sensitive methods for direct hydroperoxide measurement. We report on a technique for measuring hydroperoxide which relies upon the rapid hydroperoxide‐mediated oxidation of Fe 2+ under acidic conditions. Fe 3+ forms a chromophore with xylenol orange which absorbs strongly at 560 nm, yielding an apparent E 560 (for H 2 O 2, butyl hydroperoxide and cumene hydroperoxide) of 4.3×10 4 M−1 cm−1. The assay was validated in a study of liposomal lipid peroxidation and shown to give results comparable with those obtained by an iodometric method or by measuring conjugated dienes. The assay involving thiobarbituric acid, by comparison, underestimates lipid peroxidation and does not measure hydroperoxide per se.
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Citations
Cited by
Monteiro, D. A., de Almeida, J. A., Rantin, F. T., & Kalinin, A. L. (2006) · Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology · doi:10.1016/j.cbpc.2006.01.004 · Cited by 384
Bi, J. L., & Felton, G. W. (1995) · Journal of Chemical Ecology · doi:10.1007/bf02035149 · Cited by 376
de Pablos, R. M., Herrera, A. J., Espinosa-Oliva, A. M., Sarmiento, M., Muñoz, M. F., Machado, A., & Venero, J. L. (2014) · Journal of Neuroinflammation · doi:10.1186/1742-2094-11-34 · Cited by 201
Dietrich, J.-B., Mangeol, A., Revel, M.-O., Burgun, C., Aunis, D., & Zwiller, J. (2005) · Neuropharmacology · doi:10.1016/j.neuropharm.2005.01.018 · Cited by 168
CP-31398, a novel p53-stabilizing agent, induces p53-dependent and p53-independent glioma cell death
Wischhusen, J., Naumann, U., Ohgaki, H., Rastinejad, F., & Weller, M. (2003) · Oncogene · doi:10.1038/sj.onc.1207198 · Cited by 158
Metadata
- Authors
- Jiang, Z., Woollard, A. C. S., & Wolff, S. P.
- Venue
- Lipids
- Publisher
- Wiley
- Published
- 1991-10
- Volume
- 26
- Issue
- 10
- Pages
- 853-856
- ISSN
- 0024-4201 (ISSN-L)
- 1558-9307
- OpenAlex
- W2078843599
- Cited by
- 634
- References
- 0
- FWCI
- 3.77
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Lipid hydroperoxide measurement by oxidation of Fe2+ in the presence of xylenol orange. Comparison with the TBA assay and an iodometric method. https://app.cochat.ai/papers/10.1007/bf02536169
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- Jiang, Z., Woollard, A. C. S., & Wolff, S. P. (1991). Lipid hydroperoxide measurement by oxidation of Fe2+ in the presence of xylenol orange. Comparison with the TBA assay and an iodometric method. Lipids. https://doi.org/10.1007/bf02536169
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