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Quantitative analysis of lipids by thin‐layer chromatography
Abstract
A procedure is described for the quantitative analysis of neutral and phospholipids by thinlayer chromatography (TLC) employing densitometry. The chromatophates are prepared with the usual solvent systems. The spots are charred under standard conditions and analyzed with a Photovolt Corp. densitometer equipped with a special stage designed for holding 20ȕ20 cm chromatoplates. Each spot on the chromatoplate gives a peak of density values which is used for quantitative analysis. Radioactive lipids are analyzed by autoradiography by the densitometry of radiograms of chromatoplates developed from X‐ray films. The precision of the method is demonstrated on model mixtures of mono, di‐and triglycerides, neural and phospholipids and C 14 labeled lipids. Results of the analysis of several samples of rat liver lipids compared closely to those obtained by silicic acid column chromatography.
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[54] Thin-layer chromatography of lipids
(1969) · Methods in Enzymology · doi:10.1016/s0076-6879(69)14056-2 · Cited by 573
Complete separation of lipid classes on a single thin-layer plate
Freeman, C. P., & West, D. (1966) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)39297-x · Cited by 567
Quantitative densitometric thin-layer chromatography of lipids using copper acetate reagent
Fewster, M. E., Burns, B. J., & Mead, J. F. (1969) · Journal of Chromatography A · doi:10.1016/s0021-9673(00)99173-8 · Cited by 403
Characteristics and Composition of Watermelon, Pumpkin, and Paprika Seed Oils and Flours
El-Adawy, T. A., & Taha, K. M. (2001) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf001117+ · Cited by 259
Lipid analysis by quantitative thin‐layer chromatography
Privett, O. S., Blank, M. L., Codding, D. W., & Nickell, E. C. (1965) · Journal of the American Oil Chemists' Society · doi:10.1007/bf02635573 · Cited by 172
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Metadata
- Authors
- Blank, M. L., Schmit, J. A., & Privett, O. S.
- Venue
- Journal of the American Oil Chemists' Society
- Publisher
- Wiley
- Published
- 1964-05
- Volume
- 41
- Issue
- 5
- Pages
- 371-376
- ISSN
- 0003-021X (ISSN-L)
- 1558-9331
- OpenAlex
- W2091976327
- Cited by
- 239
- References
- 0
- FWCI
- 17.86
- Type
- journal article
- Language
- en
- Open access
- closed
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- Blank, M. L., Schmit, J. A., & Privett, O. S. (1964). Quantitative analysis of lipids by thin‐layer chromatography. Journal of the American Oil Chemists' Society. https://doi.org/10.1007/bf02654817
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