CoChat
Sign Up
Cited by 36

The DOI matches its Crossref record.

Overexpression of ROCK in human breast cancer cells: Evidence that ROCK activity mediates intracellular membrane traffic of lysosomes

Nishimura, Yukio, Itoh, Kazuyuki, Yoshioka, Kiyoko, Tokuda, Kazuo, Himeno, Masaru·2003·Pathology & Oncology Research
DOI 10.1007/bf03033750
Open this paper in CoChat — free account

Abstract

Small GTPase Rho and its downstream effectors, ROCK family of Rho-associated serine-threonine kinases, are thought to participate in cell morphology, motility, and tumor progression through regulating the rearrangement of actin cytoskeleton. Here we present evidence that transfection of human breast cancer cells with cDNA encoding a dominant active mutant of ROCK causes dispersal of lysosomal vesicles throughout the cytoplasm without perturbing the machinery of the endocytic pathway. The intracellular distribution of lysosomes and endocytosed transferrin, an early endosomal marker, were further assessed by confocal immunofluorescence microscopy. In the active ROCK transfected cells the lysosomal proteins, cathepsin D, LIMPII, and LAMP1, were found throughout the cytoplasm in dispersed small vesicles, which were accessible to the endocytosed Texas Red-labeled transferrin. 3D-image analysis of lysosomal distribution in the active ROCK transfectants revealed abundant punctate signals in the peripheral region of the basal plasma membrane. Cells expressing vector alone did not exhibit these alterations. Wortmannin, a phosphatidylinositol 3-kinase inhibitor, induced LIMPII-positive/ transferrin negative large vacuoles in the perinuclear region, and disappearence of the dispersed small vesicular structures. To our knowledge, this is the first evidence that increasing ROCK expression contributes to selective cellular dispersion of lysosomes in invasive breast cancer cells.

Overview

Integrity signals

Registry

Crossref · 10.1007/bf03033750

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 53

  1. Paterson HF, Self AJ, Garrett MD et al: Microinjection of recombinant p21rho induces rapid changes in cell morphology. J Cell Biol 111: 1001–1007, 1990. · DOI
  2. Perona R, Esteve P, Jimene, B et al: Tumorigenic activity of rho genes from Aphysia California. Oncogene 8: 1285–1292, 1993.
  3. Kishi K, Sasaki T, Kuroda S et al: Regulation of cytoplasmic division of Xenopus Embryo by rhop21 and its inhibitory GDP/GTP exchange protein (rhoGDI). J Cell Biol 120: 1187–1195, 1993. · DOI
  4. Takaishi K, Kikuchi A, Kuroda S et al; Involvement of rhop21 and its inhibitory GDP/GTP exchange protein (rhoGDI) in cell motility. Mol Cell Biol 13: 72–79, 1993 · DOI
  5. Hall, A. Small GTP-binding proteins and the regulation of the actin cytoskeleton. Annu Rev Cell Biol 10: 31–54, 1994 · DOI
  6. Prendergast GC, Khosravi-Far R, Solski PA, et al: Critical role of Rho in cell transformation by oncogenic Ras. Oncogene 10: 2289–2296, 1995
  7. Fritz G, Just I, Kaina B: Rho GTPases are over-expressed in human tumors. Int J Cancer 81: 682–687, 1999 · DOI
  8. Yoshioka K, Nakamori S, Itoh K: Overexpression of small GTP-binding protein RhoA promotes invasion of tumor cells. Cancer Res 59: 2004–2010, 1999
  9. Yoshioka K, Matsumura F, Akedo H, Itoh K: Small GTP-binding protein Rho stimulates the actomyosin system, leading to invasion of tumor cells. J Biol Chem 273: 5146–5154, 1998. · DOI
  10. Leung T, Manser E, Tan L, Lim L: A novel serine/threonine kinase binding the Ras-related RhoA GTPase which translocates the kinase to the peripheral membranes. J Biol Chem 270: 29051–29054, 1995. · DOI
  11. Itoh K, Yoshioka K, Akedo H et al: An essential part for Rho-associated kinase in the transcellular invasion of tumor cells. Nature Medicine 5: 221–225, 1999 · DOI
  12. Sloane BF, Moin K, Sameni M et al: Membrane association of cathepsin B can be induced by transfection of human breast epithelial cells with c-Ha-Ras oncogene. J Cell Sci 107: 373–384, 1994 · DOI
  13. Sameni M, Elliott E, Ziegler G et al: Cathepsin B and D are localized at the surface of human breast cancer cells. Pathol Oncol Res 1:43–53, 1995 · DOI
  14. Taniguchi S, Nishimura Y, Takahashi, T et al: Augmented excretion of procathepsin L of a fos-transferred highly metastatic cell line. Biochem Biophys Res Commun 168: 520–526, 1990 · DOI
  15. Kageshita T, Yoshii A, Kimura T et al: Biochemical and immunohistochemical analysis of cathepsins B, H, L and D in human melanocytic tumours. Arch Dermatol Res 287: 266–272, 1995 · DOI
  16. Albini A, Graf J, Kitten GT et al: 17β-Estradiol regulates and v-Ha-ras transfection constitutively enhances MCF7 breast cancer cell interactions with basement membrane. Proc Natl Acad Sci US 83: 8182–8186, 1986 · DOI
  17. Ichikawa T, Kyprianou N, Isaacs JT: Genetic instability and the acquisition of metastatic ability by rat mammary cancer cells following v-H-ras oncogene transfection. Cancer Res 50: 6349–6357, 1990.
  18. Nishimura Y, Sameni M, Sloane BF: Malignant transformation alters intracellular trafficking of lysosomal cathepsin D in human breast epithelial cells. Pathol Oncol Res 4: 283–296, 1998 · DOI
  19. Nishimura Y, Itoh K, Yoshioka, K et al: Small guanosine triphosphatase Rho/Rho-associated kinase as a novel regulator of intracellular redistribution of lysosomes in invasive tumor cells. Cell Tissue Res 342: 341–351, 2000. · DOI · Paper page
  20. Uehata M, Ishizaki T, Satoh H et al: A key role for pl60ROCK-mediated Ca++sensitization of smooth muscle in hypertension. Nature 389: 990–994, 1997. · DOI
  21. Matsui T, Amano M, Xamamoto T et al: Rho-associated kinase, a novel serine threonine kinase, as a putative target for the small GTP binding protein Rho. EMBO J 15: 2208–2216, 1996 · DOI
  22. Nakagawa O, Fujisawa K, Ishizaki et al: ROCK-I and ROCK-II; two isoforms of Rho-associated coiled-coil forming protein serine/threonine kinase in mice. FEBS Lett 392: 189–193, 1996 · DOI
  23. Amano M, Chihara K, Kimur, Y et al: Formation of actin stress fibers and focal adhesions enhanced by Rho-kinase. Science 275: 1308–1311, 1997. · DOI · Paper page
  24. Ishizaki T, Naito M, Fujisaw, K et al: pl60ROCK, a Rho-associated coiled-coil forming protein kinase, works downstream of Rho and induces focal adhesion. FEBS Lett 404: 118–124, 1997 · DOI
  25. Nishimura Y, Itoh K, Xoshioka K, et al: A role for small GTPase RhoA in regulating intracellular membrane traffic of lysosomes in invasive rat hepatoma cells. Histochem J 34: 189–213, 2002. · DOI · Paper page
  26. Lippincott-Schwart J, Fambrough DM: Lysosomal membrane dynamics: structure and interorganellar movement of a major lysosomal membrane glycoprotein. J Cell Biol 102: 1593–1605, 1986. · DOI
  27. Griffiths G, Hoflack B, Simon, K et al: The mannose 6-phosphate receptor and the biogenesis of lysosomes. Cell 52: 329–341, 1998 · DOI
  28. Kornfeld S, Mellman I: The biogenesis of lysosomes. Ann Rev Cell Biol 5: 483–525, 1989. · DOI
  29. Okazaki I, Himeno M, Ezaki J et al: Purification and characterization of an 85 kDa sialoglycoprotein in rat liver. J Biochem 111: 763–769, 1992. · DOI
  30. Sandoval IV, Arredondo JJ, Alcalde J et al: The residues Leu(Ile)475-Ile(Leu)476, contained in the extended carboxyl cytoplasmic tail, are critical for targeting of the resident lysosomal membrane protein LIMPII to lysosomes. J Biol Chem 269: 6622–6631, 1994 · DOI
  31. Tabuchi N, Akasaki K, Tsuji H: Two acidic amino acid residues, Asp(470) and Glu(471), contained in the carboxyl cytoplasmic tail of a major lysosomal membrane protein, LGP85/LIMPII, are important for its accumulation in secondary lysosomes. Biochem Biophys Res Commun 270: 557–563, 2000. · DOI
  32. Soule HD, Vazguez J, Long et al.: A human cell line from a pleural effusion derived from a breast carcinoma. J Natl Cancer Inst 51: 1409–1416, 1973. · DOI
  33. Nishimura Y, Higaki M, Kato K: Identification of a precursor form of cathepsin D in microsomal lumen: characterization of enzymatic activation and proteolytic processing in vitro. Biochem Biophys Res Commun 148: 335–343, 1987 · DOI
  34. Nishimura Y, Kawabata T, Kato K: Identification of latent procathepsins B and L in microsomal lumen: characterization of enzymatic activation and proteolytic processing in vitro. Arch Biochem Biophys 261: 64–71, 1988 · DOI
  35. Matteoni R, Kreis TE: Translocation and clustering of endosomes and lysosomes depends on microtubules. J Cell Biol 105: 1253–1265, 1987 · DOI
  36. Mu FT, Callaghan JM, Steele-Mortimer O et al: EEAl, an early endosome-associated protein. EEAl is a conserved alpha-helical peripheral membrane protein flanked by cysteine “fingers” and contains a calmodulin-binding IQ motif. J Biol Chem 270: 13503–13511, 1995 · DOI
  37. Stack JH, Emr SD: Genetic and biochemical studies of protein sorting to the yeast vacuole. Curr Biol 5: 641–646, 1993. · DOI
  38. Brown WJ Emr SD, Plutner H, Balch WE: Role for phosphatidylinositol 3-kinase in the sorting and transport of newly synthesized lysosomal enzymes in mammalian cells. J Cell Biol 130: 797–805, 1995 · DOI
  39. Davidson H: Wortmannin causes mis-targeting of procathepsin D. Evidence for the involvement of a phosphatidylinositol 3-kinase in vesicular transport to lysosomes. J Cell Biol 130: 797–805, 1995 · DOI
  40. Reaves B, Bright NA, Mullock B, Luzio JP: The effect of wortmannin on the localization of lysosomal type I integral membrane glycoproteins suggests a role for phosphoinositide 3-kinase activity in regulating membrane traffic late in the endocytic pathway. J Cell Sci 109: 749–762, 1996 · DOI
  41. Pfeffer SR: GTP-binding proteins in intracellular transport. Trends Cell Biol 2: 41–46, 1992 · DOI
  42. Zerial M, Stenmark H: Rab GTPases in vesicular transport. Curr Opin Cell Biol 5: 613–620, 1993 · DOI
  43. Chavrier P, Goud B: The role of ARF and Rab GTPases in membrane transport. Curr Opin Cell Biol, 11: 466–475, 1999 · DOI
  44. Pfeffer SR: Transport-vesicle targeting: tethers before SNAREs. Nature Cell Biol 1: E17-E22, 1999. · DOI
  45. Bucci C, Thomsen P, Nicoziani P et al: Rab7: A key to lysosome biogenesis. Mol Biol Cell 11: 467–480, 2000 · DOI
  46. Press B, Feng Y, Hoflack B, Windinger-Nes A: Mutant Rab7 causes the accumulation of cathepsin D and cation-independent mannose 6-phosphate receptor in an early endocytic compartment. J Cell Biol 140: 1075–1089, 1998. · DOI
  47. Hirose M, Ishizaki T, Watanabe N et al: Molecular dissection of the Rho-associated kinase (pl60ROCK)-regulated neurite remodeling in neuroblastoma N1E-115 cells. J Cell Biol 141: 1625–1636, 1998 · DOI
  48. Hollenbeck PJ, Swanson JA: Radial extension of macrophage tubular lysosomes supported by kinesin. Nature 346: 864–866, 1990 · DOI
  49. Nakata T, Hirokawa N: Point mutation of adenosine triphosphate-binding motif generated rigor kinesin that selectively blocks anterograde lysosome membrane transport. J Cell Biol 131: 1039–1053, 1995 · DOI
  50. Hotta K, Tanak K, Mino A, et al: Interaction of the Rho family small G proteins with kinectin, an anchoring protein of kinesin motor. Biochem Biophys Res Commun 225: 69–74, 1996 · DOI
  51. Vignal E, Blangy A, Martin M, et al: Kinectin is a key effector of RhoG microtubule-dependent cellular activity. Mol Cell Biol 21:8022–8034, 2001 · DOI
  52. Timar J, Tang D, Bazaz R, et al: PKC mediates 12-(S)-HETE-induced cytoskeletal rearrangement in B16a melanoma cells. Cell Motil Cytoskel 26: 49–65, 1993 · DOI
  53. Timar J, Bazaz R, Kimler V, et al: Immunomorphological characterization and effects of 12-(S)-HETE on a dynamic intracellular pool of the αIIbβ3-integrin in melanoma cells. J Cell Sci 108: 2175–2186, 1995 · DOI

Cited by

Sorted by citations

Metadata

Authors
Nishimura, Y., Itoh, K., Yoshioka, K., Tokuda, K., & Himeno, M.
Venue
Pathology & Oncology Research
Publisher
Springer Science and Business Media LLC
Published
2003-06
Volume
9
Issue
2
Pages
83-95
ISSN
1219-4956
DOI
10.1007/bf03033750
Cited by
36
References
0
Language
en
Licence
www.springer.com/tdm (tdm)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review