Expression of Human Growth Hormone by the Eukaryotic Alga, Chlorella
Abstract
A method to use Chlorella to express a recombinant heterologous protein that can be recovered from the extracellular medium has been developed. Plasmids are constructed with an extracellular secretion signal sequence inserted between a promoter region and a gene for human growth hormone (hGH). The plasmids also contain a Kanr region which confers resistance to the antibiotic G418. Protoplasts are prepared by enzymatic treatment, and the plasmid is introduced by incubation of the protoplasts with polyethylene glycol and dimethyl sulfoxide. Cells are then grown in the presence of G418, and the medium is collected from 6 days after transfection. hGH is measured by immunoassay, and values for expressed hGH of about 200-600 ng/ml are obtained.
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Nuclear transformation has been achieved in a number of other green algae species, such as members of the Chlorella and volvox genuses.
“while nuclear transformation has been achieved in a number of other green algae species, such as members of the Chlorella and volvox genuses [99,100].”
From Biofuels from algae: Challenges and potential (2010) · cites this paper as [100]
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References 26 · Cited by 139
- Authors
- Hawkins, & Nakamura, M.
- Affiliations
- Toyota Motor Corporation (Japan) (JP)
- ORCID
- Hawkins,, Richard L.
- Venue
- Current Microbiology
- Published
- 1999-06
- Volume
- 38
- Issue
- 6
- Pages
- 335-341
- ISSN
- 0343-8651 (ISSN-L)
- OpenAlex
- W2010476490
- Cited by
- 139
- References
- 26
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 7 · 2023: 5 · 2022: 4 · 2021: 7 · 2020: 6 · 2019: 6 · 2018: 3 · 2017: 13 · 2016: 14 · 2015: 18 · 2014: 14 · 2013: 7 · 2012: 3
- FWCI
- 0.33
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Current Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- human growth hormone, Chlorella, heterologous expression, recombinant protein secretion, protoplast transfection, polyethylene glycol-mediated transformation, G418 selection
- MeSH terms
- Amino Acid Sequence, Anti-Bacterial Agents, Chlorella, Chloroquine, Cosmids, Gentamicins, Hot Temperature, Introns, Molecular Sequence Data, Plasmids, Promoter Regions, Genetic, Transfection, Blotting, Southern, Blotting, Western, Gene Expression, Electroporation, Human Growth Hormone, Protein Sorting Signals
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