The DOI matches its Crossref record; 62 of the 62 references it cites were found in a registry or an index.
FieF (YiiP) from Escherichia coli mediates decreased cellular accumulation of iron and relieves iron stress
Abstract
The Escherichia coli yiiP gene encodes an iron transporter, ferrous iron efflux (FieF), which belongs to the cation diffusion facilitator family (CDF). Transcription of fieF correlated with iron concentration; however, expression appeared to be independent of the ferrous iron uptake regulator Fur. Absence of FieF led to decreased growth of E. coli cells in complex growth medium but only if fur was additionally deleted. The presence of EDTA was partially able to relieve this growth deficiency. Expression of fieF in trans rendered the double deletion strain more tolerant to iron. Furthermore, E. coli cells exhibited reduced accumulation of (55)Fe when FieF was expressed in trans. FieF catalyzed active efflux of Zn(II) in antiport with protons energized by NADH via the transmembrane pH gradient in everted membrane vesicles. Using the iron-sensitive fluorescent indicator PhenGreen-SK encapsulated in proteoliposomes, transmembrane fluxes of iron cations were measured with purified and reconstituted FieF by fluorescence quenching. This suggests that FieF is an iron and zinc efflux system, which would be the first example of iron detoxification by efflux in any organism.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“To date, alternative ferrous Fe import systems have been identified, including ZupT, YfeABCD, FutABC and EfeUOB 71-76 .”
From Nutritional immunity: the battle for nutrient metals at the host–pathogen interface (2022) · cites this paper as [72]
Citations
Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Molecular cloning: A laboratory manual
Deininger, P. L. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,684
CRC Handbook of Chemistry and Physics
(2014) · doi:10.1201/b17118 · Cited by 57,706
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.
Datsenko, K., & Wanner, B. (2000) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.120163297 · Cited by 15,902
CRC Handbook of Chemistry and Physics
P, D. (1992) · Journal of Molecular Structure · doi:10.1016/0022-2860(92)85083-s · Cited by 9,443
Andrews, S. C., Robinson, A. K., & Rodríguez-Quiñones, F. (2003) · FEMS Microbiology Reviews · doi:10.1016/s0168-6445(03)00055-x · Cited by 2,702
Miller, J. H. (1992) · Medical Entomology and Zoology · Cited by 2,488
Pedigrees of some mutant strains of Escherichia coli K-12
Bachmann, B. (1972) · Bacteriological Reviews · doi:10.1128/br.36.4.525-557.1972 · Cited by 1,700
Positional cloning of zebrafish ferroportin1 identifies a conserved vertebrate iron exporter
Donovan, A., Brownlie, A., Zhou, Y., Shepard, J., Pratt, S. J., Moynihan, J., Paw, B. H., Drejer, A., Barut, B., Zapata, A., et al. (2000) · Nature · doi:10.1038/35001596 · Cited by 1,667
Efflux-mediated heavy metal resistance in prokaryotes
Nies, D. H. (2003) · FEMS Microbiology Reviews · doi:10.1016/s0168-6445(03)00048-2 · Cited by 1,567
Self-citation
Cited by
Sorted by citations
Feist, A. M., Henry, C. S., Reed, J. L., Krummenacker, M., Joyce, A. R., Karp, P. D., Broadbelt, L. J., Vassily Hatzimanikatis, A., & Palsson, B. Ø. (2007) · Molecular Systems Biology · doi:10.1038/msb4100155 · Cited by 1,408
Magnetotactic Bacteria and Magnetosomes
Faivre, D., & Schüler, D. (2008) · Chemical Reviews · doi:10.1021/cr078258w · Cited by 877
Nutritional immunity: the battle for nutrient metals at the host–pathogen interface
Murdoch, C. C., & Skaar, E. P. (2022) · Nature Reviews Microbiology · doi:10.1038/s41579-022-00745-6 · Cited by 701
Coordination Chemistry of Bacterial Metal Transport and Sensing
Ma, Z., Jacobsen, F. E., & Giedroc, D. P. (2009) · Chemical Reviews · doi:10.1021/cr900077w · Cited by 643
A bacterial view of the periodic table: genes and proteins for toxic inorganic ions
Silver, S. D., & Phung, L. T. (2005) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-005-0019-6 · Cited by 525
Metadata
- Authors
- Grass, G., Otto, M., Fricke, B., Haney, C. J., Rensing, C., Nies, D. H., & Munkelt, D.
- Affiliations
- Martin Luther University Halle-Wittenberg (DE)
- University of Arizona (US)
- Corresponding author
- Grass, Gregor
- Venue
- Archives of Microbiology
- Published
- 2004-11-11
- Volume
- 183
- Issue
- 1
- Pages
- 9-18
- ISSN
- 0003-9276 (ISSN-L)
- 0302-8933
- 1432-072X
- OpenAlex
- W2104808495
- Cited by
- 233
- References
- 62
- Citations by year
- 2026: 5 · 2025: 13 · 2024: 12 · 2023: 9 · 2022: 12 · 2021: 6 · 2020: 14 · 2019: 10 · 2018: 13 · 2017: 9 · 2016: 8 · 2015: 11 · 2014: 17 · 2013: 11 · 2012: 12
- FWCI
- 5.71
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Archives of Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- National Institute of Environmental Health Sciences (ES 04940, P42ES004940)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Keywords
- fiefs, cation diffusion facilitator, Escherichia coli, iron stress, iron accumulation, Fur regulator, zinc efflux, antiport, iron detoxification, transmembrane pH gradient, proteoliposomes
- MeSH terms
- Bacterial Proteins, Biological Transport, Active, Escherichia coli, Fluorescent Dyes, Genetic Complementation Test, Hydrogen-Ion Concentration, Iron, Organic Chemicals, Repressor Proteins, Zinc, Ion Transport, Gene Deletion, Membrane Transport Proteins, Escherichia coli Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
