The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Ten commercial antibodies for alpha-1-adrenergic receptor subtypes are nonspecific
Open accessAbstract
Commercial antibodies are used widely to quantify and localize the alpha1-adrenergic receptor (AR) subtypes, alpha1A, alpha1B, and alpha1D. We tested ten antibodies, from abcam and Santa Cruz, using western blot with heart and brain tissue from wild-type (WT) mice and mice with systemic knockout (KO) of one or all three subtypes. We found that none of the antibodies detected a band in WT that was absent in the appropriate KO or in the KO that was null for all alpha1-ARs (ABDKO). We conclude that the antibodies we tested are not specific for alpha1-ARs. These results raise caution with prior studies using these reagents. For now, competition radioligand binding is the only reliable approach to quantify the alpha1-AR subtype proteins. Receptor protein localization remains a challenge.
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“Simply citing a company name and target leaves much ambiguity and, in a surprisingly large percentage of cases, leads scientists to waste money and time trying to optimize the wrong reagent [27–29].”
From Open Science 2.0: Towards a truly collaborative research ecosystem (2023) · cites this paper as [28]
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Decreased blood pressure response in mice deficient of the α 1b -adrenergic receptor
Cavalli, A., Lattion, A.-L., Hümmler, E., Nenniger-Tosato, M., Pedrazzini, T., Aubert, J.-F., Michel, M. C., Yang, M., Lembo, G., Vecchione, C., et al. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.21.11589 · Cited by 289
O’Connell, T. D., Ishizaka, S., Nakamura, A., Swigart, P. M., Rodrigo, M. C., Simpson, G. L., Cotecchia, S., Rokosh, G. D., Grossman, W., Foster, E., et al. (2003) · Journal of Clinical Investigation · doi:10.1172/jci16100 · Cited by 205
Self-citationRokosh, G. D., & Simpson, P. C. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.132552699 · Cited by 193
Self-citation1-Adrenergic receptors prevent a maladaptive cardiac response to pressure overload
O’Connell, T. D. (2006) · Journal of Clinical Investigation · doi:10.1172/jci22811 · Cited by 143
Jensen, B. C., Swigart, P. M., Laden, M.-E., DeMarco, T., Hoopes, C. W., & Simpson, P. C. (2009) · Journal of the American College of Cardiology · doi:10.1016/j.jacc.2009.05.056 · Cited by 73
Self-citationSignal transduction and regulation: Are all α1-adrenergic receptor subtypes created equal?
Hein, P., & Michel, M. C. (2006) · Biochemical Pharmacology · doi:10.1016/j.bcp.2006.11.001 · Cited by 54
α1d Adrenoceptor signaling is required for stimulus induced locomotor activity
Kreibich, A. S., Coughlin, L., Fu, H., Wang, B., Valladares, O., Valentino, R. J., & Blendy, J. A. (2003) · Molecular Psychiatry · doi:10.1038/sj.mp.4001351 · Cited by 48
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Clinical implications from studies of α1 adrenergic receptor knockout mice
Koshimizu, T., Tanoue, A., & Tsujimoto, G. (2006) · Biochemical Pharmacology · doi:10.1016/j.bcp.2006.11.002 · Cited by 13
Perez, D. M. (2006) · Humana Press eBooks · doi:10.1385/1592599311 · Cited by 13
Simpson, P. C. (2006) · Humana Press eBooks · doi:10.1385/1-59259-931-1:207 · Cited by 7
Self-citationJensen, B. C., Swigart, P. M., Myagmar, B., Shah, S. C., DeMarco, T., Hoopes, C. W., & Simpson, P. C. (2007) · Circulation · doi:10.1161/circ.116.suppl_16.ii_289 · Cited by 3
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Bordeaux, J., Welsh, A. W., Agarwal, S., Killiam, E. J., Baquero, M. T., Hanna, J. A., Anagnostou, V., & Rimm, D. L. (2010) · BioTechniques · doi:10.2144/000113382 · Cited by 597
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How reliable are G-protein-coupled receptor antibodies?
Michel, M. C., Wieland, T., & Tsujimoto, G. (2009) · Naunyn-Schmiedeberg's Archives of Pharmacology · doi:10.1007/s00210-009-0395-y · Cited by 306
O’Connell, T. D., Jensen, B. C., Baker, A. J., & Simpson, P. C. (2013) · Pharmacological Reviews · doi:10.1124/pr.112.007203 · Cited by 205
Self-citationRapid Identification of Monospecific Monoclonal Antibodies Using a Human Proteome Microarray
Jeong, J. S., Jiang, L., Albino, E., Marrero, J., Rho, H., Hu, J., Hu, S., Vera, C., Poueymiroy, D. B., Rivera-Pacheco, Z. A., et al. (2012) · Molecular & Cellular Proteomics · doi:10.1074/mcp.o111.016253 · Cited by 171
Metadata
- Authors
- Jensen, B. C., Swigart, P. M., & Simpson, P. C.
- Affiliations
- San Francisco VA Medical Center (US)
- Corresponding author
- Simpson, Paul C.
- Venue
- Naunyn-Schmiedeberg's Archives of Pharmacology
- Published
- 2008-11-07
- Volume
- 379
- Issue
- 4
- Pages
- 409-412
- ISSN
- 0028-1298 (ISSN-L)
- 1432-1912
- PMC
- PMC2653258
- OpenAlex
- W2077606276
- Cited by
- 176
- References
- 12
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 7 · 2023: 8 · 2022: 6 · 2021: 11 · 2020: 10 · 2019: 11 · 2018: 9 · 2017: 8 · 2016: 14 · 2015: 8 · 2014: 20 · 2013: 14 · 2012: 12
- FWCI
- 4.12
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Naunyn-Schmiedeberg s Archives of Pharmacology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- U.S. Department of Veterans Affairs
- National Heart, Lung, and Blood Institute (R01HL031113)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Immunology and Microbiology › Immunology › Monoclonal and Polyclonal Antibodies Research
- Keywords
- alpha1-adrenoceptors, commercial antibodies, antibody specificity, receptor knockout mice, receptor localization
- MeSH terms
- Animals, Antibodies, Antibody Specificity, Brain Chemistry, Mice, Inbred C57BL, Myocardium, Blotting, Western, Receptors, Adrenergic, alpha-1, Mice, Knockout, Mice
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