Lack of specificity of commercially available antisera against muscarinergic and adrenergic receptors
Open accessAbstract
Commercially available antisera against five subtypes of muscarinic receptors and nine subtypes of adrenoceptors showed highly distinct immunohistochemical staining patterns in rat ureter and stomach. However, using the M(1-4) muscarinic receptor subtypes and alpha(2B)-, beta(2)-, and beta(3)-adrenoceptors as examples, Western blots with membranes prepared from cell lines stably expressing various subtypes of muscarinic receptors or adrenoceptors revealed that each of the antisera recognized a set of proteins that differed between the cell lines used but lacked specificity for the claimed target receptor. We propose that receptor antibodies need better validation before they can reliably be used.
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There is uncertainty around the usefulness of antibodies against GPCRs.
“However, there is uncertainty around the usefulness of antibodies against GPCRs (Michel et al., 2009”
From Self-reported symptom burden in postural orthostatic tachycardia syndrome (POTS): A narrative review of observational and interventional studies (2023) · cites this paper as Pradidarcheep et al., 2009
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How reliable are G-protein-coupled receptor antibodies?
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“G protein-coupled receptors (GPCRs) comprise a large class of proteins that regulate many physiological functions such as vision, smell, taste, neurotransmission, cardiac output, and pain perception (83, 84).”
Influential
- Authors
- Pradidarcheep, W., Stallen, J., Labruyère, W. T., Dabhoiwala, N. F., Michel, M. C., & Lamers, W. H.
- Affiliations
- Srinakharinwirot University (TH)
- Amsterdam UMC Location University of Amsterdam (NL)
- Corresponding author
- Lamers, Wouter H.
- Venue
- Naunyn-Schmiedeberg's Archives of Pharmacology
- Published
- 2009-02-07
- Volume
- 379
- Issue
- 4
- Pages
- 397-402
- ISSN
- 0028-1298 (ISSN-L)
- 1432-1912
- OpenAlex
- W1985491990
- Cited by
- 152
- References
- 0
- Citations by year
- 2025: 3 · 2024: 8 · 2023: 7 · 2022: 2 · 2021: 1 · 2020: 6 · 2019: 4 · 2018: 10 · 2017: 8 · 2016: 4 · 2015: 10 · 2014: 18 · 2013: 11 · 2012: 13
- FWCI
- 8.02
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc
- Locations
- Naunyn-Schmiedeberg s Archives of Pharmacology (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Research Publications (Maastricht University) (publishedVersion, cc-by-nc)
- UvA-DARE (University of Amsterdam) (publishedVersion, other-oa)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Pure Amsterdam UMC (submittedVersion)
- Research Publications (Maastricht University) (submittedVersion, other-oa)
- Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Keywords
- muscarinic receptors, adrenergic receptors, antisera specificity, receptor antibodies, antibody validation, alpha2B-adrenoceptor, beta2-adrenergic receptor, beta3-adrenergic receptor, immunohistochemistry, Western blot, rat stomach
- MeSH terms
- Animals, Antibodies, Antibody Specificity, Brain Chemistry, Cell Line, Humans, Immune Sera, Immunohistochemistry, Receptors, Adrenergic, Receptors, Muscarinic, Transfection, Urinary Tract, Blotting, Western, Rats, Wistar, Gastrointestinal Tract, Rats
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