Infliximab abrogates adenine-induced chronic kidney disease via modulation of the MAPK/JNK/ASK signaling pathway in rats
Open accessAbstract
Chronic kidney disease (CKD) is a prominent cause of death worldwide. Infliximab is one of the anti-TNF-α; herein, we studied the effect of infliximab on adenine-induced CKD. To inspect the role of infliximab, either ameliorative or curative, on CDK induced with adenine. Thirty Wistar albino rats were separated into five groups of 6 rats' each: rats of group Ι were kept as control given saline, rats of group II were treated with infliximab (5 mg/kg, i.p.) for 5 weeks, rats of group ΙΙΙ (the diseased group) had an adenine containing diet (0.25% W/W in feed) for 5 weeks, rats of group ΙV (the ameliorative group) had an adenine-containing diet and infliximab (5 mg/kg, i.p.) for 5 weeks simultaneously, and rats of group V (the curative group) had adenine containing diet then a single dose of infliximab (5 mg/kg, i.p.) was given in the 6th week. Infliximab treatment revealed a decrease in the plasma levels of urea, creatinine, NGAL, and MDA with a substantial increase in TAC. Also, inflammatory mediators such as IL-6 and NF-κB were significantly decreased with the down-regulation of the ASK1/MAPK/JNK pathway. Caspase 3 was downregulated. Also, infliximab treatment exhibited improvement in the histological and immunohistochemical kidney changes. Through its involvement in reducing oxidative stress, inflammation, and apoptosis, infliximab has an ameliorative and curative effect on CKD induced with adenine.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Animal experiments have shown that TNF-α-blocking Infliximab improves renal function through attenuating fibrosis and decreasing albumin excretion in a diabetic nephropathy rat model.
“Furthermore, animal experiments have shown that TNF-α-blocking Infliximab improves renal function through attenuating fibrosis 20 and decreasing albumin excretion in a diabetic nephropathy rat model, 21,22”
From The effect of <scp>GLP</scp> ‐1 receptor agonists on circulating inflammatory markers in type 2 diabetes patients: A systematic review and meta‐analysis (2025) · cites this paper as [20]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
No references could be resolved yet.
Research works printed but not linked: 41
- Abdelrahman AM Al Suleimani YM Manoj P Effect of infliximab, a tumor necrosis factor-alpha inhibitor, on doxorubicin-induced nephrotoxicity in rats Naunyn Schmiedebergs Arch Pharmacol 2020 393 1 121 130 10.1007/s00210-019-01719-x 31501914 · DOI
- Ali BH Mohammed A Sirin AA The effect of sildenafil on rats with adenine—induced chronic kidney disease Biomed Pharmacother 2018 108 391 402 10.1016/j.biopha.2018.09.061 30236848 · DOI
- Ali BH Al Salam S Al Suleimani Y Effects of the SGLT-2 inhibitor canagliflozin on adenine-induced chronic kidney disease in rats Cell Physiol Biochem 2019 52 1 27 39 10.33594/000000003 30790503 · DOI
- Cai H Su S Li Y Protective effects of Salvia miltiorrhiza on adenine-induced chronic renal failure by regulating the metabolic profiling and modulating the NADPH oxidase/ROS/ERK and TGF-β/Smad signaling pathways J Ethno Pharmacol 2018 212 153 165 10.1016/j.jep.2017.09.021 29032117 · DOI
- Cheng X Shen Y Li R Targeting TNF: a therapeutic strategy for Alzheimer’s disease Drug Discov 2014 19 1822 1827 24998784
- Corremans R Neven E Maudsley S Progression of established non-diabetic chronic kidney disease is halted by metformin treatment in rats Kidney Int 2022 101 5 929 944 10.1016/j.kint.2022.01.037 35271933 · DOI
- Dadsetan S Balzano T Forteza J Reducing peripheral inflammation with infliximab reduces neuroinflammation and improves cognition in rats with hepatic encephalopathy Front Mol Neurosci 2016 9 106 10.3389/fnmol.2016.00106 27853420 PMC5089983 · DOI
- Dawson-Saunders B, Trapp R (2001) Basic, and clinical biostatics, 3rd edn. Lang Medical Book, McGraw Hill Medical Publishing Division, New York, 161–218
- Diwan V Mistry A Gobe G Adenine-induced chronic kidney and cardiovascular damage in rats Epub 2013 68 2 197 207 23732959
- Elenkov IJ Iezzoni DG Daly A Cytokine dysregulation, inflammation, and well-being NeuroImmunoModulation 2005 12 255 269 10.1159/000087104 16166805 · DOI
- Elzbieta S Krzysztof JC Maria KM Helena D Agnieszka M Effect of infliximab on the levels of TNF-alpha and TGF-beta in the whole blood cultures of irradiated patients Folia Histochem Cytobiol 2008 46 3 291 297 19056532
- Eto N Wada T Inagi R Podocyte protection by darbepoetin: preservation of the cytoskeleton and nephrin expression Kidney Int 2007 72 455 463 10.1038/sj.ki.5002311 17457371 · DOI
- Farris AB Colvin RB Renal interstitial fibrosis: mechanisms and evaluation Curr Opin Nephrol Hypertens 2012 21 289 300 10.1097/MNH.0b013e3283521cfa 22449945 PMC3354760 · DOI
- Fawcett JK Scott JE A rapid and precise method for the determination of urea J Clin Pathol 1960 13 2 156 159 10.1136/jcp.13.2.156 13821779 PMC480024 · DOI
- Gong Q, He LL, Wang ML, Ouyang H, Gao H, Feng Y, Yang S, Du L, Li J, Luo Y (2019) Anemoside B4 protects rat kidney from adenine-induced injury by attenuating inflammation and fibrosis and enhancing podocin and nephrin expression. Evid-Based Complement Alternat Med 11 PMC6582884 31275420
- Gori P Patel A Nilay Solanki N Protective effects of lycopene against adenine-induced chronic renal failure in rats Indian J Physiol Pharmacol 2021 65 2 74 85 10.25259/IJPP_188_2020 · DOI
- Haiting H Xu L Yanwu C Inhibition of TRPC6 signal pathway alleviates podocyte injury induced by TGF-β1 Cell Physiol Biochem 2017 41 1 163 172 10.1159/000455985 28214865 · DOI
- Hsu PK Powe NR Recent trends in the prevalence of chronic kidney disease: not the same old song Curr Opin Nephrol Hypertens 2017 26 187 196 10.1097/MNH.0000000000000315 28319485 · DOI
- Ito SY Eri Manabe Yi Dai Juzentaihoto improves adenine-induced chronic renal failure in BALB/c mice via suppression of renal fibrosis and inflammation J Pharmacol Sci 2022 184 1 172 78 10.1016/j.jphs.2021.10.009 34924123 · DOI
- Kato N Matsumoto M Kogawa M Critical role of p38 MAPK for regeneration of the sciatic nerve following crush injury in vivo J Neuroinflammation 2013 10 757 10.1186/1742-2094-10-1 PMC3541116 23282009 · DOI
- Kim HJ Vaziri ND Norris K High-calorie diet with moderate protein restriction prevents cachexia and ameliorates oxidative stress, inflammation, and proteinuria in experimental chronic kidney disease Clin Exp Nephrol 2010 14 536 547 10.1007/s10157-010-0340-x 20820841 PMC3206096 · DOI
- Koracevic D Koracevic G Djordjevic V Method for the measurement of antioxidant activity in human fluids J Clin Pathol 2001 54 356 361 10.1136/jcp.54.5.356 11328833 PMC1731414 · DOI
- Livak KJ Schmittgen TD Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Δ Δ C (T)) method Methods (San Diego, Calif) 2001 25 4 402 8 10.1006/meth.2001.1262 11846609 · DOI · Paper page
- Malek M Nematbakhsh M Renal ischemia/reperfusion injury; from pathophysiology to treatment J Renal Inj Prev 2015 4 20 27 26060833 PMC4459724
- Mohamad HE Abo-Elmatty DM Wahba NS Infliximab and/or MESNA alleviate doxorubicin-induced Alzheimer’s disease-like pathology in rats: a new insight into TNF-α/Wnt/β-catenin signaling pathway Life Sci 2022 3 301 120613 10.1016/j.lfs.2022.120613 35523286 · DOI
- Nishitoh H Saitoh M Mochida Y Takeda K Nakano H Rothe M Miyazono K Ichijo H ASK1 is essential for JNK/SAPK activation by TRAF2 Mol Cell 1998 2 3 389 395 10.1016/S1097-2765(00)80283-X 9774977 · DOI
- Pisoni R Wille K Tolwani A The epidemiology of severe acute kidney injury: from BEST to PICARD, in acute kidney injury: new concepts Nephron Clin Pract 2008 109 4 188 191 10.1159/000142927 18802366 · DOI
- Pollock I Rampling D Greenwal SE Desmin expression in rhabdomyosarcoma: influence of the desmin clone and immunohistochemical method J Clin Pathol 1995 48 6 535 38 10.1136/jcp.48.6.535 7665696 PMC502683 · DOI
- Poutoglidou F Pourzitaki C Manthou ME Effects of long-term infliximab and tocilizumab treatment on anxiety-like behavior and cognitive function in naive rats Pharmacol Rep 2022 74 1 84 95 10.1007/s43440-021-00328-x 34569017 · DOI
- Qin W Xu Z Lu Y Mixed organic solvents induce renal injury in rats PLoS ONE 2012 7 9 10.1371/journal.pone.0045873 PMC3448683 23029287 · DOI
- Raftery G He J Pearce R Disease activity and cognition in rheumatoid arthritis: an open label pilot study Arthritis Res Ther 2012 14 263 10.1186/ar4108 PMC3674605 23211089 · DOI
- Ramos-Vara JA Kiupel M Baszler T Suggested guidelines for immunohistochemical techniques in veterinary diagnostic laboratories J Vet Diagn Invest 2008 20 393 413 10.1177/104063870802000401 18599844 · DOI
- Saritemur M Un H Cadirci E Tnf-α inhibition by infliximab as a new target for the prevention of glycerol-contrast-induced nephropathy Environ Toxicol Pharmacol 2015 39 2 577 588 10.1016/j.etap.2015.01.002 25682004 · DOI
- Satoh K Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method Clin Chim Acta 1978 90 1 37 43 10.1016/0009-8981(78)90081-5 719890 · DOI
- Schirmeister J Willmann H Kiefer H Plasma creatinine as rough indicator of renal function Dtsch Med Wochenschr 1964 22 89 1018 1023 10.1055/s-0028-1111251 14135304 · DOI
- Tasdemir C Tasdemir S Vardi N Protective effect of infliximab on ischemia/reperfusion-induced damage in rat kidney Ren Fail 2012 34 1144 1149 10.3109/0886022X.2012.717490 22950848 · DOI
- Triantafillidis JK Papalois AE Parasi A Favorable response to subcutaneous administration of infliximab in rats with experimental colitis World J Gastroenterol 2005 11 43 6843 6847 10.3748/wjg.v11.i43.6843 16425394 PMC4725043 · DOI
- Yokozawa T Zheng PD Oura H Animal model of adenine-induced chronic renal failure in rats Nephron 1986 44 230 234 10.1159/000183992 3785486 · DOI
- Younis NN, Mohamed HE, Shaheen MA, Abdelghafour AM, Hammad SK (2021) Inactivation of Wnt/β-catenin/renin-angiotensin axis by tumor necrosis factor-alpha inhibitor, infliximab, ameliorates CKD induced in rats. Biochem Pharmacol 185 33482150
- Zhang HF Wang YL Gao C Salvianolic acid A attenuates kidney injury and inflammation by inhibiting NF-κB and p38 MAPK signaling pathways in 5/6 nephrectomized rats Acta Pharmacol Sin 2018 39 12 1855 1864 10.1038/s41401-018-0026-6 29795135 PMC6289371 · DOI
- Zoccali C Vanholder R Massy ZA European renal and cardiovascular medicine (EURECA-m) working group of the European Renal Association – European Dialysis Transplantation Association (ERA-EDTA), The systemic nature of CKD Nat Rev Nephrol 2017 13 344 358 10.1038/nrneph.2017.52 28435157 · DOI
Cited by
Sorted by citations
Ren, Y., Chen, Y., Zheng, W., Kong, W., Liao, Y., Zhang, J., Wang, M., & Zeng, T. (2025) · Diabetes, Obesity and Metabolism · doi:10.1111/dom.16366 · Cited by 59 · see citing works
- Authors
- Nageeb, M. M., Talaat, A., Reda, S. M., & Elsammak, G. A.
- Affiliations
- Zagazig University (EG)
- Corresponding author
- Nageeb, Mahitab M.
- Venue
- Naunyn-Schmiedeberg's Archives of Pharmacology
- Published
- 2023-07-04
- Volume
- 397
- Issue
- 1
- Pages
- 207-219
- ISSN
- 0028-1298 (ISSN-L)
- 1432-1912
- PMC
- PMC10771379
- OpenAlex
- W4383058352
- Cited by
- 7
- References
- 0
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 1
- FWCI
- 1.36
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Naunyn-Schmiedeberg s Archives of Pharmacology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Greater South Information System
- Funders
- Zagazig University
- Topics
- Health Sciences › Medicine › Nephrology › Chronic Kidney Disease and Diabetes
- Health Sciences › Medicine › Nephrology › Acute Kidney Injury Research
- Health Sciences › Medicine › Nephrology › Dialysis and Renal Disease Management
- Keywords
- infliximab, chronic kidney disease, adenine-induced nephropathy, anti-TNF therapy, oxidative stress, inflammation, apoptosis, caspase-3, NF-κB, interleukin-6, nitric oxide synthase, malondialdehyde, neutrophil gelatinase-associated lipocalin
- MeSH terms
- Infliximab, Tumor Necrosis Factor Inhibitors, Adenine, Animals, Kidney, Rats, Wistar, Oxidative Stress, MAP Kinase Signaling System, Rats, Renal Insufficiency, Chronic
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
