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Cited by 678RNA Interference and Gene DeliveryMolecular Biology Techniques and ApplicationsViral Infectious Diseases and Gene Expression in Insects

Mammalian cell transfection: the present and the future

Open access
Kim, Tae Kyung, Eberwine, James H.·2010·Analytical and Bioanalytical Chemistry
DOI 10.1007/s00216-010-3821-6PMC PMC2911531
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Abstract

Transfection is a powerful analytical tool enabling study of the function of genes and gene products in cells. The transfection methods are broadly classified into three groups; biological, chemical, and physical. These methods have advanced to make it possible to deliver nucleic acids to specific subcellular regions of cells by use of a precisely controlled laser-microscope system. The combination of point-directed transfection and mRNA transfection is a new way of studying the function of genes and gene products. However, each method has its own advantages and disadvantages so the optimum method depends on experimental design and objective.

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  • The heterogeneity in plasmid transfection is probably due to the size of the plasmid (~5000 bp) compared to siRNA (20 bp).

    “This is probably due to the size of the plasmid (∼5000 bp) when compared to siRNA (20 bp), as previously described (6).”

    From Direct transfection of clonal organoids in Matrigel microbeads: a promising approach toward organoid-based genetic screens (2018) · cites this paper as [6]

    The Methodological diversity section mentions variable transfection efficiency by cell type or condition, and Transfection of RNAs discusses siRNA delivery. Neither passage attributes heterogeneity in plasmid transfection to plasmid size relative to siRNA, or gives the claimed ~5000 bp and 20 bp figures. Searches for that comparison and both sizes found no supporting passage; page numbers were unavailable in our copy. (LLM, conf=0.73)

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Research works printed but not linked: 37

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Cited by

Sorted by citations

Authors
Kim, T. K., & Eberwine, J. H.
Affiliations
University of Pennsylvania (US)
Corresponding author
Eberwine, James H.
ORCID
Eberwine, James H.
Venue
Analytical and Bioanalytical Chemistry
Publisher
Springer Science and Business Media LLC
Published
2010-06-13
Volume
397
Issue
8
Pages
3173-3178
ISSN
  • 1618-2642 (ISSN-L)
  • 1618-2650
DOI
10.1007/s00216-010-3821-6
PMC
PMC2911531
OpenAlex
W2090693843
Cited by
678
References
1
Citations by year
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FWCI
3.13
Type
journal article
Language
en
Open access
Licence
cc-by-nc
Locations
  • Analytical and Bioanalytical Chemistry (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
Keywords
mammalian cell transfection, nucleic acid delivery, mRNA transfection, gene function analysis
MeSH terms
Animals, Cell Line, Genetic Vectors, Humans, Mammals, Transfection

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