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Cited by 19Lipid Membrane Structure and BehaviorBacterial Genetics and BiotechnologyLipid metabolism and biosynthesis

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Long-Term Adaptation of Bacillus subtilis 168 to Extreme pH Affects Chemical and Physical Properties of the Cellular Membrane

Petrackova, Denisa, Vecer, Jaroslav, Svobodova, Jaroslava, Herman, Petr·2010·Journal of Membrane Biology
DOI 10.1007/s00232-010-9226-9
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Abstract

We characterized physical and chemical properties of cell-membrane fragments from Bacillus subtilis 168 (trpC2) grown at pH 5.0, 7.0 and 8.5. Effects of long-term bacterial adaptation reflected in growth rates and in changes of the membrane lipid composition were correlated with lipid order and dynamics using time-resolved fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene. We demonstrate that the pH adaptation results in a modification of a fatty acid content of cellular membranes that significantly influences both the lipid-chain order and dynamics. For cultivation at acidic conditions, the lipid order increases and membrane dynamics decreases compared to pH 7.0. This results in rigid and ordered membranes. Cultivation at pH 8.5 causes slight membrane disordering. Instant pH changes induce qualitatively similar but smaller effects. Proton flux measurements performed on intact cells adapted to both pH 5.0 and 8.5 revealed lower cell-membrane permeability compared to bacteria cultivated at pH optimum. Our results indicate that both acidic and alkalic pH stress represent a permanent challenge for B. subtilis to keep a functional membrane state. The documented adaptation-induced adjustments of membrane properties could be an important part of mechanisms maintaining an optimal intracellular pH at a wide range of extracellular proton concentrations.

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Metadata

Authors
Petrackova, D., Vecer, J., Svobodova, J., & Herman, P.
Venue
Journal of Membrane Biology
Publisher
Springer Science and Business Media LLC
Published
2010-02
Volume
233
Issue
1-3
Pages
73-83
ISSN
  • 0022-2631 (ISSN-L)
  • 1432-1424
DOI
10.1007/s00232-010-9226-9
OpenAlex
W1968051911
Cited by
19
References
0
Citations by year
2026: 1 · 2025: 2 · 2024: 1 · 2021: 1 · 2020: 1 · 2018: 3 · 2017: 1 · 2016: 1 · 2015: 2 · 2014: 2 · 2013: 1 · 2012: 1
FWCI
0.49
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • The Journal of Membrane Biology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords
Bacillus subtilis 168, pH adaptation, bacterial membranes, membrane lipid composition, fatty acid content, lipid chain order, membrane dynamics, membrane permeability, proton flux, intracellular pH homeostasis
MeSH terms
Bacillus subtilis, Cell Membrane, Diphenylhexatriene, Fluorescence Polarization, Hydrogen-Ion Concentration

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