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Cited by 144Mercury impact and mitigation studiesHeavy Metal Pollution RemediationAquaculture disease management and microbiota

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Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction

Zhang, Weiwei, Chen, Lingxin, Liu, Dongyan·2012·Applied Microbiology and Biotechnology
DOI 10.1007/s00253-011-3454-5
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Abstract

The Pseudomonas putida strain SP1 was isolated from marine environment and was found to be resistant to 280 μM HgCl₂. SP1 was also highly resistant to other metals, including CdCl₂, CoCl₂, CrCl₃, CuCl₂, PbCl₂, and ZnSO₄, and the antibiotics ampicillin (Ap), kanamycin (Kn), chloramphenicol (Cm), and tetracycline (Tc). mer operon, possessed by most mercury-resistant bacteria, and other diverse types of resistant determinants were all located on the bacterial chromosome. Cold vapor atomic absorption spectrometry and a volatilization test indicated that the isolated P. putida SP1 was able to volatilize almost 100% of the total mercury it was exposed to and could potentially be used for bioremediation in marine environments. The optimal pH for the growth of P. putida SP1 in the presence of HgCl₂ and the removal of HgCl₂ by P. putida SP1 was between 8.0 and 9.0, whereas the optimal pH for the expression of merA, the mercuric reductase enzyme in mer operon that reduces reactive Hg²⁺ to volatile and relatively inert monoatomic Hg⁰ vapor, was around 5.0. LD₅₀ of P. putida SP1 to flounder and turbot was 1.5 × 10⁹ CFU. Biofilm developed by P. putida SP1 was 1- to 3-fold lower than biofilm developed by an aquatic pathogen Pseudomonas fluorescens TSS. The results of this study indicate that P. putida SP1 is a low virulence strain that can potentially be applied in the bioremediation of HgCl₂ contamination over a broad range of pH.

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Metadata

Authors
Zhang, W., Chen, L., & Liu, D.
Affiliations
Chinese Academy of Sciences (CN)
Corresponding author
Chen, Lingxin
ORCID
Venue
Applied Microbiology and Biotechnology
Publisher
Springer Science and Business Media LLC
Published
2011-07-13
Volume
93
Issue
3
Pages
1305-1314
ISSN
  • 0175-7598 (ISSN-L)
  • 1432-0614
DOI
10.1007/s00253-011-3454-5
OpenAlex
W2167639117
Cited by
144
References
0
Citations by year
2026: 6 · 2025: 6 · 2024: 13 · 2023: 13 · 2022: 13 · 2021: 14 · 2020: 6 · 2019: 9 · 2018: 9 · 2017: 9 · 2016: 12 · 2015: 16 · 2014: 8 · 2013: 5 · 2012: 5
FWCI
3.89
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Applied Microbiology and Biotechnology (publishedVersion)
  • PubMed (publishedVersion)
  • Knowledge Repository of Yantai Institute of Coastal Zone Research, CAS (Yantai Institute of Coastal Zone Research) (submittedVersion)
Funders
  • National Natural Science Foundation of China
  • Chinese Academy of Sciences
Topics
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Mercury impact and mitigation studies
  • Physical Sciences › Environmental Science › Water Science and Technology › Heavy Metal Pollution Remediation
  • Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
Keywords
Pseudomonas putida, mercury resistance, mer operon, mercuric reductase, mercury volatilization, mercury bioremediation, metal resistance, antibiotic resistance, biofilm, virulence
MeSH terms
Animals, Anti-Bacterial Agents, Biodegradation, Environmental, Fish Diseases, Flatfishes, Flounder, Hydrogen-Ion Concentration, Mercuric Chloride, Mercury, Microbial Sensitivity Tests, Molecular Sequence Data, Operon, Oxidoreductases, Pseudomonas Infections, RNA, Ribosomal, 16S, Seawater, Volatilization, Pseudomonas putida, Sequence Analysis, DNA, Drug Resistance, Bacterial

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