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Cited by 32Bacterial Genetics and BiotechnologyDrug Transport and Resistance MechanismsATP Synthase and ATPases Research

Role of the Group 2 Mrp sodium/proton antiporter in rapid response to high alkaline shock in the alkaline- and salt-tolerant Dietzia sp. DQ12-45-1b

Fang, Hui, Qin, Xiao-Yu, Zhang, Kai-Duan, Nie, Yong, Wu, Xiao-Lei·2018·Applied Microbiology and Biotechnology
DOI 10.1007/s00253-018-8846-3
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Abstract

The six- and seven-subunit Na + /H + antiporters (Mrp) are widely distributed in bacteria. They are reported to be integral for pH homeostasis in alkaliphilic bacteria when adapting to high pH environments. In this study, operons encoding for the six-subunit Na + /H + antiporters were found in the genomes of all studied Dietzia strains, which have different alkaline-resistant abilities. Disruption of the operon in the strain Dietzia sp. DQ12-45-1b which leads to declined growth in presence of hypersaline and alkaline conditions suggested that the six-subunit Na + /H + antiporter played an important role in hypersaline and alkaline resistance. Although the complexes DqMrp from DQ12-45-1b (strain with high alkaline resistance) and DaMrp from D. alimentaria 72 T (strain with low alkaline resistance) displayed Na + (Li + )/H + antiport activities, they functioned optimally at different pH levels (9.0 for DQ12-45-1b and 8.0 for 72 T ). While both antiporters functioned properly to protect Escherichia coli cells from salt shock, only the DqMrp-containing strain survived the high alkaline shock. Furthermore, real-time PCR results showed that the expression of mrpA and mrpD induced only immediately after DQ12-45-1b cells were subjected to the alkaline shock. These results suggested that the expression of DqMrp might be induced by a pH gradient across the cell membrane, and DqMrp mainly functioned at an early stage to respond to the alkaline shock.

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Cited by

Sorted by citations

Authors
Fang, H., Qin, X.-Y., Zhang, K.-D., Nie, Y., & Wu, X.-L.
Affiliations
Peking University (CN)
Corresponding author
  • Nie, Yong
  • Wu, Xiao-Lei
ORCID
Venue
Applied Microbiology and Biotechnology
Publisher
Springer Science and Business Media LLC
Published
2018-03-03
Volume
102
Issue
8
Pages
3765-3777
ISSN
  • 0175-7598 (ISSN-L)
  • 1432-0614
DOI
10.1007/s00253-018-8846-3
OpenAlex
W2791576242
Cited by
32
References
0
Citations by year
2026: 4 · 2025: 2 · 2024: 5 · 2023: 2 · 2022: 7 · 2021: 3 · 2020: 6 · 2019: 2 · 2018: 1
FWCI
1.81
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Applied Microbiology and Biotechnology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (31225001, 31300108)
  • Ministry of Science and Technology of the People's Republic of China (2014CB846002)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › ATP Synthase and ATPases Research
Keywords
Na+/H+ antiporter, MRP, alkaliphilic bacteria, Dietzia, pH homeostasis, salt tolerance, MrpA, gene induction
MeSH terms
Hydrogen-Ion Concentration, Operon, Stress, Physiological, Gene Expression Regulation, Bacterial, Sodium-Hydrogen Exchangers, Actinobacteria

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