The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Bacillus subtilis Endospores at High Purity and Recovery Yields: Optimization of Growth Conditions and Purification Method
Abstract
Bacillus subtilis endospores have applications in different fields including their use as probiotics and antigen delivery vectors. Such specialized applications frequently require highly purified spore preparations. Nonetheless, quantitative data regarding both yields and purity of B. subtilis endospores after application of different growth conditions and purification methods are scarce or poorly reported. In the present study, we conducted several quantitative and qualitative analyses of growth conditions and purification procedures aiming generation of purified B. subtilis spores. Based on two growth media and different incubations conditions, sporulation frequencies up to 74.2 % and spore concentrations up to 7 × 10(9) spores/ml were achieved. Application of a simplified spore isolation method, in which samples were incubated with lysozyme and a detergent, resulted in preparations with highly purified spores at the highest yields. The present study represents, therefore, an important contribution for those working with B. subtilis endospores for different biotechnological purposes.
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“Various flask-scale investigations have reported spore yields ranging from approximately 1 × 10 6 to 7 × 10 9 spores/mL [7-10,20].”
From Investigation of a Broad-Bean Based Low-Cost Medium Formulation for Bacillus subtilis MSCL 897 Spore Production (2023) · cites this paper as [8]
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Cites
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Self-citationOptimizing Bacillus subtilis spore isolation and quantifying spore harvest purity
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Cited by
Effect of Medium Components and Culture Conditions in Bacillus subtilis EA-CB0575 Spore Production
Posada-Uribe, L. F., Romero-Tabarez, M., & Villegas-Escobar, V. (2015) · Bioprocess and Biosystems Engineering · doi:10.1007/s00449-015-1428-1 · Cited by 106
Enhanced Spore Production of Bacillus subtilis Grown in a Chemically Defined Medium
Monteiro, S. M. S., Clemente, J. J., Carrondo, M. J. T., & Cunha, A. E. (2014) · Advances in Microbiology · doi:10.4236/aim.2014.48049 · Cited by 50
Abuhena, M., Al-Rashid, J., Azim, M. F., Khan, M. N. M., Kabir, M. G., Barman, N. C., Rasul, N. M., Akter, S., & Huq, M. A. (2022) · Scientific Reports · doi:10.1038/s41598-022-15366-5 · Cited by 50
Bacillus Based Microbial Formulations: Optimization of the Production Process
Stamenkovic-Stojanovic, S., Karabegovic, I., Beskoski, V., Nikolic, N., & Lazic, M. (2019) · Hemijska industrija · doi:10.2298/hemind190214014s · Cited by 37
Grigs, O., Didrihsone, E., & Bolmanis, E. (2023) · Fermentation · doi:10.3390/fermentation9040390 · Cited by 7
Metadata
- Authors
- Tavares, M. B., Souza, R. D., Luiz, W. B., Cavalcante, R. C. M., Casaroli, C., Martins, E. G., Ferreira, R. C. C., & Ferreira, L. C. S.
- Affiliations
- Universidade de São Paulo (BR)
- Venue
- Current Microbiology
- Published
- 2012-11-27
- Volume
- 66
- Issue
- 3
- Pages
- 279-285
- ISSN
- 0343-8651 (ISSN-L)
- 1432-0991
- OpenAlex
- W1992009956
- Cited by
- 64
- References
- 15
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 7 · 2023: 9 · 2022: 7 · 2021: 5 · 2020: 4 · 2019: 3 · 2018: 6 · 2017: 2 · 2016: 1 · 2015: 6 · 2014: 3 · 2013: 3
- FWCI
- 2.30
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Current Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacillus and Francisella bacterial research
- Keywords
- Bacillus subtilis, endospores, spore purification, spore yield, growth conditions, lysozyme treatment, detergent treatment, spore isolation, probiotics
- MeSH terms
- Bacillus subtilis, Culture Media, Spores, Bacterial, Time Factors
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
