On the Iron Requirement of Lactobacilli Grown in Chemically Defined Medium
Abstract
The iron requirement of four strains of lactobacilli (L. acidophilus, L. delbrueckii subsp. bulgaricus, L. plantarum, and L. pentosus) was studied in a synthetic medium under aerobic or anaerobic conditions. Effects of iron salt and iron-chelated compounds were tested on bacterial growth in manganese-free or -supplemented media. No significant growth stimulation was observed in any condition. These results support the absolute manganese requirement for optimum growth of lactobacilli and the needless incorporation of iron in growth media.
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A few organisms have dispensed with an iron requirement altogether, including lactic acid bacteria and Borrelia burgdorferi.
“A few organisms seem to have dispensed with an iron requirement altogether, including the lactic acid bacteria92,93 and the pathogenic spirochete Borrelia burgdorferi.94”
From Metals in Motion: Understanding Labile Metal Pools in Bacteria (2025) · cites this paper as [93]
Cites
Iron requirement and search for siderophores in lactic acid bacteria
Pandey, A., Bringel, F., & Meyer, J.-M. (1994) · Applied Microbiology and Biotechnology · doi:10.1007/bf00173337 · Cited by 117 · see citing works
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Kortman, G. A. M., Raffatellu, M., Swinkels, D. W., & Tjalsma, H. (2014) · FEMS Microbiology Reviews · doi:10.1111/1574-6976.12086 · Cited by 291 · see citing works
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Dostal, A., Fehlbaum, S., Chassard, C., Zimmermann, M. B., & Lacroix, C. (2013) · FEMS Microbiology Ecology · doi:10.1111/j.1574-6941.2012.01461.x · Cited by 129 · see citing works
Parmanand, B. A., Kellingray, L., Le Gall, G., Basit, A. W., Fairweather-Tait, S., & Narbad, A. (2019) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2019.01.010 · Cited by 110 · see citing works
Genetic modification of iron metabolism in mice affects the gut microbiota
Buhnik-Rosenblau, K., Moshe-Belizowski, S., Danin-Poleg, Y., & Meyron-Holtz, E. G. (2012) · BioMetals · doi:10.1007/s10534-012-9555-5 · Cited by 53 · see citing works
A Heme-responsive Regulator Controls Synthesis of Staphyloferrin B in Staphylococcus aureus
Laakso, H. A., Marolda, C. L., Pinter, T. B., Stillman, M. J., & Heinrichs, D. E. (2016) · Journal of Biological Chemistry · doi:10.1074/jbc.m115.696625 · Cited by 52 · see citing works
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Helmann, J. D. (2025) · Biochemistry · doi:10.1021/acs.biochem.4c00726 · Cited by 49 · see citing works
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- Authors
- Imbert, M., & Blondeau, R.
- Affiliations
- Université de Lille (FR)
- Venue
- Current Microbiology
- Published
- 1998-07-01
- Volume
- 37
- Issue
- 1
- Pages
- 64-66
- ISSN
- 0343-8651 (ISSN-L)
- 1432-0991
- OpenAlex
- W2049257708
- Cited by
- 102
- References
- 1
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 6 · 2023: 5 · 2022: 5 · 2021: 6 · 2020: 1 · 2019: 8 · 2018: 2 · 2017: 5 · 2016: 5 · 2015: 5 · 2014: 6 · 2013: 4 · 2012: 9
- FWCI
- 0.71
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Current Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Keywords
- lactobacilli, iron requirements, manganese requirement, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus plantarum, Lactobacillus pentosus, chemically defined medium, iron chelators, iron salts, bacterial growth, aerobic and anaerobic conditions
- MeSH terms
- Aerobiosis, Anaerobiosis, Bacteriological Techniques, Culture Media, Iron, Iron Chelating Agents, Lactobacillus, Magnesium Sulfate
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