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Endopolygalacturonase genes and enzymes from Saccharomyces cerevisiae and Kluyveromyces marxianus
Open accessAbstract
The gene encoding endopolygalacturonase (EC 3.2.1.15) has been cloned, sequenced and expressed from three strains of Saccharomyces cerevisiae (including nonsecretors) and three strains of Kluyveromyces marxianus. Both control and coding regions showed small differences within each species, one including loss of a potential glycosylation site. Two non-secreting S. cerevisiae strains (FY1679 and var. uvarum) had non-transcribed copies of functional genes. Maximum enzyme activity was achieved with the S. cerevisiae FY1679 gene in an expressing vector, with an enzyme activity of 51 micromol of reducing sugar released from polygalacturonic acid microg protein(-1) min(-1), the highest so far reported for a yeast.
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“Bacteria have various advantages over filamentous fungi as they do not experience rheological issues in fermentation broth and do not produce a mixture of pectinases [24].”
From Screening and optimization of extracellular pectinase produced by Bacillus thuringiensis SH7 (2023) · cites this paper as [24]
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Research works printed but not linked: 4
- [13]13. Nelson NJ (1957) Colorimetric analysis of sugars. In: Colowick SP, Kaplan NO (eds) Methods in enzymology, vol 3. Academic Press, New York, pp 85±86
- [17]17. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning (a laboratory manual). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
- [20]20. Schwan RF, Cooper RM, Rose AH, Wheals AE (1996) Endo- polygalacturonase of the yeast Kluyveromyces marxianus is constitutive, highly active on native pectin and is the main ex- tracellular protein. In: Visser J, Voragen AGJ (eds) Progress in biotechnology 14: pectins and pectinases. Elsevier Press, Amsterdam, pp 861±868
- [24]24. Visser J, Voragen AGJ (1996) Progress in biotechnology 14: pectins and pectinases. Elsevier, Amsterdam
Cited by
The hemibiotrophic lifestyle of Colletotrichum species
Münch, S., Lingner, U., Floss, D. S., Ludwig, N., Sauer, N., & Deising, H. B. (2008) · Journal of Plant Physiology · doi:10.1016/j.jplph.2007.06.008 · Cited by 233
Screening and optimization of extracellular pectinase produced by Bacillus thuringiensis SH7
Umar, M., Rehman, A., Khan, I., Hayat, P., Hayat, A., Rehman, M. U., Shah, T. A., Dawoud, T. M., Hadrach, S., & Bourhia, M. (2023) · Open Chemistry · doi:10.1515/chem-2022-0358 · Cited by 8
“filamentous fungi as they do not experience rheological issues in fermentation broth and do not produce a mixture of pectinases [24].”
Background
Metadata
- Authors
- Jia, J., & Wheals, A.
- Affiliations
- University of Bath (GB)
- ORCID
- Jia, Jianhua
- Venue
- Current Genetics
- Published
- 2000-12
- Volume
- 38
- Issue
- 5
- Pages
- 264-270
- ISSN
- 0172-8083 (ISSN-L)
- 1432-0983
- OpenAlex
- W1974170353
- Cited by
- 33
- References
- 0
- Citations by year
- 2025: 1 · 2023: 1 · 2021: 1 · 2019: 2 · 2018: 1 · 2017: 1 · 2016: 3 · 2014: 1 · 2013: 3 · 2012: 2
- FWCI
- 0.36
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Current Genetics (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
- Keywords
- endopolygalacturonase, Saccharomyces cerevisiae, Kluyveromyces marxianus, gene cloning, gene expression, glycosylation sites, polygalacturonic acid, reducing sugar release, yeast enzymes
- MeSH terms
- Amino Acid Sequence, Cloning, Molecular, Kluyveromyces, Molecular Sequence Data, Polygalacturonase, Recombinant Proteins, Saccharomyces cerevisiae, Species Specificity
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