Amelioration of dextran sulfate sodium-induced colitis by neuropeptide Y antisense oligodeoxynucleotide
Abstract
Background Neuropeptide Y (NPY) from enteric neurons has been shown to play an important role in immune and inflammatory responses. The purpose of the present study was to investigate the effects of NPY antisense oligodeoxynucleotides (ODNs) on an experimental model of ulcerative colitis (UC).Methods NPY antisense ODNs were administered in experimental colitis induced by dextran sulfate sodium (DSS). The disease activity index (DAI) and histological score were observed. The tumor necrosis factor (TNF)-alpha and NPY levels were measured by enzyme-linked immunosorbent assay. Phosphorylated Akt (p-Akt) expression was determined by immunohistochemical staining. Activated nuclear factor (NF)-kappaB was assessed by western blot analysis. Myeloperoxidase (MPO) activity was determined by using MPO assay kit.Results A significant improvement was observed in DAI and histological score in rats with NPY antisense ODNs, and the increase in NPY and TNF-alpha levels, MPO activity, and the expression p-Akt and p-NF-kappaB in rats with DSS-induced colitis was significantly reduced following the administration of NPY antisense ODNs.Conclusion The administration of NPY antisense ODNs leads to an amelioration of DSS-induced colitis, suggesting that NPY plays an important role in modulating inflammation in colitis, and NPY antisense ODNs may be a useful therapeutic approach to the treatment of UC.
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In a colitis disease model, it was found that an increase in the colonic synthesis of NPY was associated with decreased colonic Y1 receptor expression.
“For example, in a colitis disease model, it was found that an increase in the colonic synthesis of NPY was associated with decreased colonic Y 1 receptor expression [208,209].”
From Anxiety, depression, and the microbiome: A role for gut peptides (2018) · cites this paper as [209]
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References 28 · Cited by 25
- Authors
- Pang, X. H., Li, T. K., Xie, Q., He, F. Q., Cui, D. J., Chen, Y. Q., Huang, X. L., & Gan, H. T.
- Affiliations
- Sichuan University (CN)
- Dali University (CN)
- Corresponding author
- Gan, Hua Tian
- Venue
- International Journal of Colorectal Disease
- Published
- 2010-06-05
- Volume
- 25
- Issue
- 9
- Pages
- 1047-1053
- ISSN
- 0179-1958 (ISSN-L)
- 1432-1262
- OpenAlex
- W2087843844
- Cited by
- 25
- References
- 28
- Citations by year
- 2025: 1 · 2019: 2 · 2017: 3 · 2016: 3 · 2015: 6 · 2014: 2 · 2013: 2 · 2012: 2
- FWCI
- 1.26
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- International Journal of Colorectal Disease (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Inflammatory Bowel Disease
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Health Sciences › Medicine › Epidemiology › Microscopic Colitis
- Keywords
- neuropeptide Y, antisense oligodeoxynucleotides, ulcerative colitis, DSS-induced colitis, inflammatory bowel disease, TNF-α, NF-κB, Akt signaling, myeloperoxidase, colonic inflammation, enteric neurons
- MeSH terms
- Animals, Colitis, Intestinal Mucosa, Male, Peroxidase, Neuropeptide Y, Oligodeoxyribonucleotides, Phosphorylation, Tumor Necrosis Factor-alpha, Signal Transduction, Dextran Sulfate, Fluorescein-5-isothiocyanate, Rats, Sprague-Dawley, Phosphatidylinositol 3-Kinases, Proto-Oncogene Proteins c-akt, Rats, Transcription Factor RelA
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