Localization of the Na + -d-glucose cotransporter SGLT1 in the blood-brain barrier
Abstract
Immunoreactivity of the Na+-D-glucose cotransporter SGLT1 was demonstrated in intracerebral capillaries of rat and pig. Immunostaining suggested that SGLT1 is located in the luminal membrane of the endothelial cells and in intracellular vesicles. Using in situ hybridization, SGLT1 mRNA was not detectable in intracerebral capillaries of non-treated or sham-operated Wistar rats. However, 1 day after a transient occlusion of the right middle cerebral artery, SGLT1 mRNA was detected in capillaries of both brain hemispheres. Expression of SGLT1 was also demonstrated in primary cultures of capillary endothelial cells from pig using polymerase chain reaction after reverse transcription and western blotting. The data suggest that SGLT1 participates in transport of D-glucose across the blood-brain barrier and is upregulated after brain ischemia and reperfusion.
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Endothelial cells also express sodium glucose cotransporter 1 (SGLT1).
“Endothelial cells also express sodium glucose cotransporter 1 (SGLT1) (159) that is found in neurons (671), but its physiological role in glucose transport across the BBB remains elusive.”
From Blood-Brain Barrier: From Physiology to Disease and Back (2019) · cites this paper as [159]
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References 31 · Cited by 82
- Authors
- Elfeber, K., K�hler, A., Lutzenburg, M., Osswald, C., Galla, H.-J., Witte, O. W., & Koepsell, H.
- Affiliations
- University of Würzburg (DE)
- Düsseldorf University Hospital (DE)
- University of Münster (DE)
- Corresponding author
- Koepsell, Hermann
- Venue
- Histochemistry and Cell Biology
- Published
- 2004-03-01
- Volume
- 121
- Issue
- 3
- Pages
- 201-207
- ISSN
- 0948-6143 (ISSN-L)
- 1432-119X
- OpenAlex
- W1991091534
- Cited by
- 82
- References
- 31
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 5 · 2022: 2 · 2021: 5 · 2020: 5 · 2019: 3 · 2018: 3 · 2017: 3 · 2016: 7 · 2015: 2 · 2014: 8 · 2013: 1 · 2012: 2
- FWCI
- 3.16
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Histochemistry and Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- Topics
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Keywords
- SGLT1, blood-brain barrier, glucose transport, capillary endothelial cells, luminal membrane, intracellular vesicles, middle cerebral artery occlusion, cerebral ischemia, reperfusion, cerebral ischemia-reperfusion injury, rats, pigs
- MeSH terms
- Animals, Blood-Brain Barrier, Cells, Cultured, Glucose, Immunohistochemistry, Male, Membrane Glycoproteins, Monosaccharide Transport Proteins, Swine, Up-Regulation, Rats, Wistar, Endothelial Cells, Sodium-Glucose Transporter 1, Rats
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