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Cited by 9Photoreceptor and optogenetics researchIon Transport and Channel RegulationIon channel regulation and function

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Combining different ion-selective channelrhodopsins to control water flux by light

Open access
Lin, Fei, Tang, Ruijing, Zhang, Chong, Scholz, Nicole, Nagel, Georg, Gao, Shiqiang·2023·Pflügers Archiv - European Journal of Physiology
DOI 10.1007/s00424-023-02853-5PMC PMC10730689

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Abstract

Water transport through water channels, aquaporins (AQPs), is vital for many physiological processes including epithelial fluid secretion, cell migration and adipocyte metabolism. Water flux through AQPs is driven by the osmotic gradient that results from concentration differences of solutes including ions. Here, we developed a novel optogenetic toolkit that combines the light-gated anion channel Gt ACR1 either with the light-gated K + channel Hc KCR1 or the new Na + channelrhodopsin Hc NCR1 with high Na + permeability, to manipulate water transport in Xenopus oocytes non-invasively. Water efflux through AQP was achieved by light-activating K + and Cl - efflux through Hc KCR1 and Gt ACR1. Contrarily, when Gt ACR1 was co-expressed with Hc NCR1, inward movement of Na + and Cl - was light-triggered, and the resulting osmotic gradient led to water influx through AQP1. In sum, we demonstrate a novel optogenetic strategy to manipulate water movement into or out of Xenopus oocytes non-invasively. This approach provides a new avenue to interfere with water homeostasis as a means to study related biological phenomena across cell types and organisms.

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Metadata

Authors
Lin, F., Tang, R., Zhang, C., Scholz, N., Nagel, G., & Gao, S.
Affiliations
  • University of Würzburg (DE)
  • Fujian Medical University (CN)
  • Leipzig University (DE)
Corresponding author
Gao, Shiqiang
ORCID
Venue
Pflügers Archiv - European Journal of Physiology
Publisher
Springer Science and Business Media LLC
Published
2023-09-05
Volume
475
Issue
12
Pages
1375-1385
ISSN
  • 0031-6768 (ISSN-L)
  • 1432-2013
DOI
10.1007/s00424-023-02853-5
PMC
PMC10730689
OpenAlex
W4386456499
Cited by
9
References
1
Citations by year
2026: 3 · 2025: 3 · 2024: 1 · 2023: 2
FWCI
1.09
Type
journal article
Language
en
Open access
Licence
Locations
  • Pflügers Archiv - European Journal of Physiology (publishedVersion, cc-by)
  • Research Square (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Qucosa (Saxon State and University Library Dresden) (submittedVersion, cc-by)
Funders
Julius-Maximilians-Universität Würzburg
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Photoreceptor and optogenetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
Keywords
aquaporins, water transport, optogenetics, osmotic gradient, water flux, Xenopus oocytes, light-gated ion channels
MeSH terms
Channelrhodopsins, Biological Transport, Oocytes, Permeability, Water, Aquaporins

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