Combined effect of ligament stem cells and umbilical-cord-blood-derived CD34+ cells on ligament healing
Abstract
Transplantation of ligament-tissue-derived stem cells has become a promising approach in the repair of injured ligament. Neovascularization plays an important role in ligament healing and remodeling. Recently, human umbilical-cord-blood-derived CD34+ cells have been reported to contribute to neoangiogenesis. Therefore, we performed a series of experiments to test our hypothesis that the combination of medial collateral ligament stem cells (MCL-SCs) and umbilical-cord-blood-derived CD34+ cells has synergistic effects on tendon healing. MCL-SCs and umbilical-cord-blood-derived CD34+ cells were isolated and cultured. Rat MCL injury was treated by MCL-SCs and/or CD34+ cells. Response to the cell therapy was assessed by gross observation, histological evaluation and biomechanical testing at 2 and 4 weeks after each treatment. Although each cell therapy group induced macroscopic and morphological recovery in healing MCLs, the combined use of MCL-SCs/CD34+ cells led to further improvement in healing quality. Capillary density was significantly higher in the CD34+ cell transplantation groups than in the other groups at week 2. Biomechanical testing demonstrated that the failure load of the healing ligament was greatest in the combination therapy group. The combination of MCL-SCs and CD34+ cells as a cell therapeutic thus enhances healing and restores biomechanical function toward normal after MCL injury. The findings obtained in our study suggest that the combination of MCL-SCs and C…
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In a rat model of MCL injury, concurrent administration of MCL-MSCs and CD34+ cells showed improved ligament healing, characterized by grossly diminished swelling, more regularly aligned fibers, increased type I collagen deposition and neovascularization, and higher tensile strength.
“After MCL injury was treated by transplantation of MCL-MSCs and/or CD34+ cells, the results demonstrated that the group with concurrent administration of MCL-MSCs and CD34+ cells showed improved ligament healing, characterized by grossly diminished swelling, more regularly aligned fibers, increased type I collagen deposition and neovascularization, and higher tensile strength.”
From Mesenchymal Stem Cells for Enhanced Healing of the Medial Collateral Ligament of the Knee Joint (2023) · cites this paper as [64]
Transplantation of a combination of MCL-MSCs and CD34+ cells could represent a promising therapeutic strategy for MCL injury.
“The authors concluded that transplantation of a combination of MCL-MSCs and CD34+ cells could represent a promising therapeutic strategy for MCL injury.”
From Mesenchymal Stem Cells for Enhanced Healing of the Medial Collateral Ligament of the Knee Joint (2023) · cites this paper as [64]
Cites
Tei, K., Matsumoto, T., Mifune, Y., Ishida, K., Sasaki, K., Shoji, T., Kubo, S., Kawamoto, A., Asahara, T., Kurosaka, M., et al. (2008) · Stem Cells · doi:10.1634/stemcells.2007-0671 · Cited by 78 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Mesenchymal Stem Cells for Enhanced Healing of the Medial Collateral Ligament of the Knee Joint
Lee, C.-S., Jeon, O.-H., Han, S.-B., & Jang, K.-M. (2023) · Medicina · doi:10.3390/medicina59040725 · Cited by 7 · see citing works
- Authors
- Jiang, D., Yang, S., Gao, P., Zhang, Y., Guo, T., Lin, H., & Geng, H.
- Affiliations
- Shanghai Jiao Tong University (CN)
- Harbin Medical University (CN)
- Corresponding author
- Geng, Hongquan
- Venue
- Cell and Tissue Research
- Published
- 2015-07-30
- Volume
- 362
- Issue
- 3
- Pages
- 587-595
- ISSN
- 0302-766X (ISSN-L)
- 1432-0878
- OpenAlex
- W977976844
- Cited by
- 20
- References
- 1
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 3 · 2023: 2 · 2021: 2 · 2020: 1 · 2019: 4 · 2018: 1 · 2017: 2
- FWCI
- 1.03
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Cell and Tissue Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Tendon Structure and Treatment
- Health Sciences › Medicine › Urology › Periodontal Regeneration and Treatments
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Keywords
- ligament healing, medial collateral ligament injury, neoangiogenesis, neovascularization, capillary density, failure load, tissue engineering
- MeSH terms
- Animals, Biomechanical Phenomena, Cell Survival, Female, Fetal Blood, Flow Cytometry, Humans, Male, Rabbits, Stem Cells, Wound Healing, Rats, Sprague-Dawley, Medial Collateral Ligament, Knee, Neovascularization, Physiologic, Antigens, CD34, Recovery of Function, Collagen Type I, Stem Cell Transplantation, Cell Shape
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
