Control of apple blue mold by Pichia pastoris recombinant strains expressing cecropin A
Abstract
Recombinant Pichia pastoris yeasts expressing cecropin A (GS115/CEC), was evaluated for the control of the blue mold of apple caused by Penicillium expansum due to cecropin A peptide's effective antimicrobial effects on P. expansum spores by the thiazolyl blue (MTT) assay. Then, the protein concentration was determined and it was expressed at high levels up to 14.2 mg/L in the culture medium. Meanwhile, the population growth was assayed in vivo. The population growth of recombinant strain GS115/CEC was higher than that of non-transformed strain GS115 in red Fuji apples wounds. Recombinant yeast strains GS115/CEC significantly inhibited growth of germinated P. expansum spores in vitro and inhibited decay development caused by P. expansum in apple fruits in vivo when compared with apple fruits inoculated with sterile water or the yeast strain GS115/pPIC (plasmid pPIC9k transformed in GS115). This study demonstrated the potential of expression of the antifungal peptide in yeast for the control of postharvest blue mold infections on pome fruits.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Cecropin A was also expressed in Pichia pastoris, generating a strain controlling apple blue mold caused by P. expansum.
“Cecropin A was also expressed in Pichia pastoris, generating a strain controlling apple blue mold caused by P. expansum (Ren et al. 2012).”
From Biocontrol yeasts: mechanisms and applications (2019) · cites this paper as Ren et al. 2012
The citation lists haven’t been fetched yet
References 31 · Cited by 11
- Authors
- Ren, X., Kong, Q., Wang, H., Yu, T., Tang, Y.-J., Zhou, W.-W., & Zheng, X.
- Affiliations
- Shihezi University (CN)
- Zhejiang University (CN)
- Corresponding author
- Zhou, Wen-Wen
- Zheng, Xiaodong
- Venue
- Bioprocess and Biosystems Engineering
- Published
- 2011-11-23
- Volume
- 35
- Issue
- 5
- Pages
- 761-767
- ISSN
- 1615-7591 (ISSN-L)
- 1615-7605
- OpenAlex
- W2080305202
- Cited by
- 11
- References
- 31
- Citations by year
- 2024: 1 · 2022: 1 · 2020: 1 · 2019: 2 · 2018: 2 · 2017: 1 · 2016: 3
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Bioprocess and Biosystems Engineering (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Postharvest Quality and Shelf Life Management
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
- Keywords
- Penicillium expansum, cecropin A, Pichia pastoris, recombinant yeast, antifungal peptides, postharvest disease control, fruit decay, wound colonization, spore germination inhibition
- MeSH terms
- Penicillium, Pichia, Plant Diseases, Recombinant Proteins, Gene Expression, Antimicrobial Cationic Peptides, Malus
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
