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Cited by 19Enzyme Catalysis and ImmobilizationEnzyme Production and CharacterizationBiofuel production and bioconversion

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Economical production of isomaltulose from agricultural residues in a system with sucrose isomerase displayed on Bacillus subtilis spores

Zhan, Yijing, Zhu, Ping, Liang, Jinfeng, Xu, Zheng, Feng, Xiaohai, Liu, Yi, Xu, Hong, Li, Sha·2020·Bioprocess and Biosystems Engineering
DOI 10.1007/s00449-019-02206-6
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Abstract

A safe, efficient, environmentally friendly process for producing isomaltulose is needed. Here, the biocatalyst, sucrose isomerase (SIase) from Erwinia rhapontici NX-5, displayed on the surface of Bacillus subtilis 168 spores (food-grade strain) was applied for isomaltulose production. The anchored SIase showed relatively high bioactivity, suggesting that the surface display system using CotX as the anchoring protein was successful. The stability of the anchored SIase was also significantly better. Thermal stability analysis showed that 80% of relative activity was retained after incubation at 40 °C and 45 °C for 60 min. To develop an economical industrial fermentation medium, untreated beet molasses (30 g/L) and cold-pressed soybean powder (50 g/L) were utilised as the main broth components for SIase pilot-scale production. Under the optimal conditions, the productive spores converted 92% of sucrose after 6 h and the conversion rate was 45% after six cycles. Isomaltulose production with this system using the agricultural residues, untreated beet molasses and soybean powder, as substrates is cost-effective and environmentally friendly and can help to overcome issues due to the genetic background.

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Metadata

Authors
Zhan, Y., Zhu, P., Liang, J., Xu, Z., Feng, X., Liu, Y., Xu, H., & Li, S.
Affiliations
Nanjing Tech University (CN)
Corresponding author
  • Xu, Hong
  • Li, Sha
ORCID
Venue
Bioprocess and Biosystems Engineering
Publisher
Springer Science and Business Media LLC
Published
2019-09-24
Volume
43
Issue
1
Pages
75-84
ISSN
  • 1615-7591 (ISSN-L)
  • 1615-7605
DOI
10.1007/s00449-019-02206-6
OpenAlex
W2976599452
Cited by
19
References
41
Citations by year
2026: 2 · 2025: 3 · 2024: 2 · 2023: 3 · 2022: 4 · 2021: 3 · 2020: 2
FWCI
0.74
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Bioprocess and Biosystems Engineering (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture (grant No. XTB1804)
  • State Key Laboratory of Materials-Oriented Chemical Engineering (grant No. ZK201606)
  • National Key R&D Program of China (2017YFD0400403)
  • National Natural Science Foundation of China (21878152)
  • Natural Science Foundation of Jiangsu Province (BK20160985)
  • Six Talent Peaks Project in Jiangsu Province and Qing Lan Project. (grant No. SWYY-027)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
  • Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
Keywords
sucrose isomerase, Bacillus subtilis spores, surface display, Erwinia rhapontici, sugar beet molasses, soybean flour, agricultural residues, enzyme thermostability
MeSH terms
Bacillus subtilis, Erwinia, Fungal Proteins, Glucosyltransferases, Hot Temperature, Isomaltose, Spores, Bacterial, Sucrose

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