Expression of sapovirus virus-like particles in mammalian cells
Abstract
Sapovirus (SaV) is an etiological agent of acute gastroenteritis in human and swine. SaV can be divided into five genogroups, GI to GV. Virus-like particles (VLPs) morphologically similar to native SaV have been expressed for GI, GII, GIII and GV strains in insect cells, although only low expression levels were observed for GII strains. In this study, we report the successful expression of SaV GII VLPs using cultured mammalian COS-7 and 293T cells. Our results demonstrated that this mammalian expression system was able to express and form SaV VLPs.
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Expression of a recombinant form of the major capsid protein is sufficient for the assembly of virus-like particles (VLPs), which exhibit the same morphology as virions.
“As is the case for many members of the Caliciviridae, expression of a recombinant form of the major capsid protein is sufficient for the assembly of virus-like particles (VLPs), which exhibit the same morphology as virions [26–29].”
From Vesivirus 2117 capsids more closely resemble sapovirus and lagovirus particles than other known vesivirus structures (2017) · cites this paper as [29]
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Conley, M., Emmott, E., Orton, R., Taylor, D., Carneiro, D. G., Murata, K., Goodfellow, I. G., Hansman, G. S., & Bhella, D. (2017) · Journal of General Virology · doi:10.1099/jgv.0.000658 · Cited by 8 · see citing works
Self-citation
- Authors
- Oka, T., Hansman, G. S., Katayama, K., Ogawa, S., Nagata, N., Miyamura, T., & Takeda, N.
- Affiliations
- National Institute of Infectious Diseases (JP)
- Venue
- Archives of Virology
- Published
- 2005-08-23
- Volume
- 151
- Issue
- 2
- Pages
- 399-404
- ISSN
- 0003-9012 (ISSN-L)
- 0304-8608
- 1432-8798
- OpenAlex
- W2049446688
- Cited by
- 16
- References
- 0
- Citations by year
- 2024: 1 · 2022: 1 · 2020: 1 · 2016: 1 · 2015: 1 · 2012: 2
- FWCI
- 0.85
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Archives of Virology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Health Sciences Foundation
- Topics
- Health Sciences › Medicine › Infectious Diseases › Viral gastroenteritis research and epidemiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Keywords
- sapovirus, virus-like particles, mammalian cell expression systems, COS-7 cells, HEK293T cells, acute gastroenteritis, pigs, insect cell expression
- MeSH terms
- Animals, Cell Line, Chlorocebus aethiops, Humans, Virus Replication, Gene Expression Regulation, Viral, Sapovirus
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