The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.
Solute accumulation in the deep-sea bacterium Photobacterium profundum
Abstract
The identity and amounts of intracellular solutes in the deep-sea bacterium Photobacterium profundum strain SS9 were studied using nuclear magnetic resonance techniques. P. profundum strain SS9, a moderate piezophile which grows optimally at 20-30 MPa primarily accumulated glutamate and betaine, with lesser amounts of alanine, beta-hydroxybutyrate (beta-HB) and oligomers composed of the beta-HB units when grown at 0.1 MPa to early stationary phase. When grown at the optimal pressure, the cells preferentially increased intracellular concentrations of beta-HB and beta-HB oligomers, while the amino acid pools remained relatively constant. Since the organic solutes increased with increasing external NaCl in the medium, they are functioning as osmolytes. The beta-HB molecules represent a novel class of osmolytes, termed 'piezolytes,' whose cellular levels responded to hydrostatic pressure as well as osmotic pressure. Factors such as cell growth stage and temperature were also examined for their effect on the solute distribution in these cells.
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“Recently, another mechanism for pressure adaptation has been hypothesized involving “piezolytes” (11,15): small organic solutes that counteract the effects of pressure on proteins, potentially allowing proteins to work over greater depth ranges but possibly also constraining species depths if regulation of these solutes is limited (11,(13)(14)(15)(16)(17).”
From Marine fish may be biochemically constrained from inhabiting the deepest ocean depths (2014) · cites this paper as [15]
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Self-citationAdaptations To High Hydrostatic Pressure
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Yancey, P. H. (2005) · Journal of Experimental Biology · doi:10.1242/jeb.01730 · Cited by 1,868
Organic compatible solutes of halotolerant and halophilic microorganisms
Roberts, M. F. (2005) · Saline Systems · doi:10.1186/1746-1448-1-5 · Cited by 665
Self-citationMethé, B. A., Nelson, K. E., Deming, J. W., Momen, B., Melamud, E., Zhang, X., Moult, J., Madupu, R., Nelson, W. C., Dodson, R. J., et al. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0504766102 · Cited by 560
Bio-mining the microbial treasures of the ocean: New natural products
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Marine fish may be biochemically constrained from inhabiting the deepest ocean depths
Yancey, P. H., Gerringer, M. E., Drazen, J. C., Rowden, A. A., & Jamieson, A. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1322003111 · Cited by 284
Metadata
- Authors
- Martin, D., Bartlett, D., & Roberts, M.
- Affiliations
- Boston College (US)
- Scripps Institution of Oceanography (US)
- Corresponding author
- Bartlett, Douglas
- Venue
- Extremophiles
- Published
- 2002-12-01
- Volume
- 6
- Issue
- 6
- Pages
- 507-514
- ISSN
- 1431-0651 (ISSN-L)
- 1433-4909
- OpenAlex
- W2026086591
- Cited by
- 133
- References
- 51
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 5 · 2023: 8 · 2022: 10 · 2021: 10 · 2020: 14 · 2019: 5 · 2018: 9 · 2017: 9 · 2016: 6 · 2015: 9 · 2014: 6 · 2013: 6 · 2012: 5
- FWCI
- 1.95
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Extremophiles (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Physical Sciences › Environmental Science › Ecology › Polar Research and Ecology
- Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
- Keywords
- Photobacterium profundum, osmolytes, piezophiles, hydrostatic pressure, glutamate, betaine, beta-hydroxybutyrate, amino acid pool, sodium chloride, growth stages, temperature effects, deep-sea bacteria
- MeSH terms
- Adaptation, Physiological, Alanine, Betaine, Biological Transport, Hydrostatic Pressure, Intracellular Fluid, Models, Biological, Photobacterium, Saline Solution, Hypertonic, Temperature, Water Microbiology, Water-Electrolyte Balance, Glutamic Acid, Nuclear Magnetic Resonance, Biomolecular, 3-Hydroxybutyric Acid
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