Downregulation of miR-23a-3p improves cognitive function in rats after subarachnoid hemorrhage by targeting VCAN
Abstract
Subarachnoid hemorrhage (SAH) is a complicated and deadly disorder. Dysregulation of miRNAs in SAH has been widely reported. This investigation elucidated the function of miR-23a-3p in the in vivo and in vitro models of SAH. The miR-23a-3p and VCAN levels in SAH rats and sham controls were detected by RT-qPCR. The SAH rats were intracerebrally administrated with miR-23a-3p antagomir. Morphological changes and brain function were assessed. The isolated brain microvascular endothelial cells (BMECs), identified by immunofluorescence staining, were used as the model of SAH in vitro. The viability and apoptosis of BMECs were evaluated using MTT, flow cytometry, and western blotting analyses. Targeted relationship between miR-23a-3p and VCAN was predicted in miRDB and validated by a luciferase reporter assay. We found that the miR-23a-3p level was upregulated in rats after SAH, while VCAN was downregulated. Silencing miR-23a-3p attenuated neurological deficits and neuronal apoptosis in rats after SAH. VCAN was verified to be targeted by miR-23a-3p. Functionally, miR-23a-3p downregulation or VCAN overexpression inhibited BMEC apoptosis and promoted cell activity. Moreover, knockdown of VCAN eliminated the influence of miR-23a-3p inhibition in BMECs. Overall, suppression of miR-23a-3p improves cognitive function after SAH by targeting VCAN.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Up-regulated expression of miR-23a-3p has been identified in blood samples of acute cerebral hemorrhage patients and in a rat model of subarachnoid hemorrhage, leading to augmented apoptosis of cerebral vascular endothelial cells by suppressing ZO-1, and formation of cerebral hematoma.
“Up-regulated expression of miR-23a-3p has been identified both in the blood samples of acute cerebral hemorrhage patients and in a rat model of subarachnoid hemorrhage, leading to augmented apoptosis of cerebral vascular endothelial cells by suppressing the expression of the target gene ZO-1, as well as the formation of cerebral hematoma.”
From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [65]
Suppression of miR-23a-3p can inhibit apoptosis of cerebral microvascular endothelial cells by targeting VCAN, and promote cell activity and improve cognitive function after subarachnoid hemorrhage in rats.
“Meanwhile, the suppression of miR-23a-3p can inhibit the apoptosis of cerebral microvascular endothelial cells by targeting VCAN, and thereafter promote cell activity and improve cognitive function after subarachnoid hemorrhage in rats [64,65].”
From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [65]
Cites
Blood-Brain Barrier: From Physiology to Disease and Back
Sweeney, M. D., Zhao, Z., Montagne, A., Nelson, A. R., & Zlokovic, B. V. (2019) · Physiological Reviews · doi:10.1152/physrev.00050.2017 · Cited by 2,294 · cited 1× · see citing works
“It is well known that maintenance of BBB integrity is essential for central nervous system (CNS) homeostasis [37].”
Background
Flagged first, then by citations
Cited by
Sorted by citations
Recent advances of miR-23 in human diseases and growth development
Qian, X., Jiang, Y., Yang, Y., Zhang, Y., Xu, N., Xu, B., Pei, K., Yu, Z., & Wu, W. (2025) · Non-coding RNA Research · doi:10.1016/j.ncrna.2024.12.010 · Cited by 5 · see citing works
- Authors
- Xue, C., Wang, R., & Jia, Y.
- Corresponding author
- Xue, Cheng
- ORCID
- Venue
- Medical Molecular Morphology
- Published
- 2022-02-08
- Volume
- 55
- Issue
- 2
- Pages
- 146-157
- ISSN
- 0918-4287 (ISSN-L)
- 1860-1480
- 1860-1499
- OpenAlex
- W4210947668
- Cited by
- 3
- References
- 1
- Citations by year
- 2024: 3
- FWCI
- 0.32
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Medical Molecular Morphology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Health Sciences › Medicine › Neurology › Intracranial Aneurysms: Treatment and Complications
- Health Sciences › Medicine › Neurology › Intracerebral and Subarachnoid Hemorrhage Research
- Keywords
- subarachnoid hemorrhage, miR-23a-3p, versican, microRNA, cognitive function, neuronal apoptosis, brain microvascular endothelial cells, antagomir, neuroprotection, microRNA target regulation
- MeSH terms
- Animals, Cognition, Subarachnoid Hemorrhage, Down-Regulation, MicroRNAs, Endothelial Cells, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
