Transcriptome analysis of probiotic Lactobacillus casei Zhang during fermentation in soymilk
Open accessAbstract
Lactobacillus casei Zhang is a widely recognized probiotic bacterium, which is being commercially used in China. To study the gene expression dynamics of L. casei Zhang during fermentation in soymilk, a whole genome microarray was used to screen for differentially expressed genes when grown to the lag phase, the late logarithmic phase, and the stationary phase. Comparisons of different transcripts next to each other revealed 162 and 63 significantly induced genes in the late logarithmic phase and stationary phase, of which the expression was at least threefold up-regulated and down-regulated, respectively. Approximately 38.4% of the up-regulated genes were associated with amino acid transport and metabolism notably for histidine and lysine biosynthesis, followed by genes/gene clusters involved in carbohydrate transport and metabolism, lipid transport and metabolism, and inorganic ion transport and metabolism. The analysis results suggest a complex stimulatory effect of soymilk-based ecosystem on the L. casei Zhang growth. On the other hand, it provides the very first insight into the molecular mechanism of L. casei strain for how it will adapt to the protein-rich environment.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
These genes may contribute to environmental adaptability and antimicrobial-based competition by maintaining metal ion homeostasis and modulating stress response mechanisms.
“They may contribute to environmental adaptability and antimicrobial-based competition by maintaining metal ion homeostasis and modulating stress response mechanisms [39].”
From Comparative Genomics-Based Evaluation of <i>Lacticaseibacillus rhamnosus</i> KFOM 0134 as a Probiotic against <i>Staphylococcus aureus</i> (2026) · cites this paper as [39]
The “Inorganic ion transport and metabolism” section links altered genes to ion homeostasis, and the “Stress response” section links gene expression to acid adaptation. The paper also lists antimicrobial-peptide transport genes under defense mechanisms, but it does not establish that the genes contribute to antimicrobial-based competition. (LLM, conf=0.84)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
The citation lists haven’t been fetched yet
References 63 · Cited by 27
- Authors
- Wang, J.-C., Zhang, W.-Y., Zhong, Z., Wei, A.-B., Bao, Q.-H., Zhang, Y., Sun, T.-S., Postnikoff, A., Meng, H., & Zhang, H.-P.
- Affiliations
- grid.411638.9 0000000417569607 Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, School of Food Science and Engineering Inner Mongolia Agricultural University 010018 Huhhot China
- Inner Mongolia Agricultural University (CN)
- Shanghai Jiao Tong University (CN)
- Corresponding author
- Meng, He
- Venue
- Journal of Industrial Microbiology and Biotechnology
- Publisher
- Oxford University Press (OUP)
- Published
- 2012-01-01
- Volume
- 39
- Issue
- 1
- Pages
- 191-206
- ISSN
- 1367-5435 (ISSN-L)
- 1476-5535
- OpenAlex
- W2003674075
- Cited by
- 27
- References
- 63
- Citations by year
- 2026: 1 · 2025: 2 · 2023: 1 · 2022: 2 · 2021: 2 · 2020: 3 · 2018: 1 · 2017: 3 · 2016: 2 · 2015: 3 · 2014: 3 · 2013: 4
- FWCI
- 3.27
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Journal of Industrial Microbiology & Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (863 Program, 30860219)
- Ministry of Education of the People's Republic of China (IRT0967)
- Earmarked Fund for Modern Agro-industry Technology Research System (nycytx-0501)
- National High-tech Research and Development Program
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Keywords
- Lactobacillus casei Zhang, probiotic bacteria, soy milk fermentation, transcriptome, gene expression dynamics, growth phase, amino acid transport, histidine biosynthesis, lysine biosynthesis, carbohydrate metabolism, lipid metabolism, inorganic ion transport
- MeSH terms
- Amino Acids, Animals, Carbon, China, Fermentation, Lacticaseibacillus casei, Gene Expression Regulation, Bacterial, Ion Transport, Probiotics, Gene Expression Profiling, Soy Milk, Lipid Metabolism
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
