Microarray analysis reveals similarity between CD8+CD28- T cells from young and elderly persons, but not of CD8+CD28+ T cells
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We isolated highly purified CD8+CD28+ and CD8+CD28- T cell populations from healthy young and elderly persons for gene expression profiling using Affymetrix oligonucleotide microarrays. We demonstrate that the gene expression profile of CD8+CD28- T cells is very similar in young and elderly persons. In contrast, CD8+CD28+ in elderly differ from CD8+CD28+ in young persons. Hierarchical clustering revealed that CD8+CD28+ in elderly are located between CD8+CD28+ in young and CD8+CD28- (young and old) T cells regarding their differentiation state. Our study demonstrates a dichotomy of gene expression levels between CD8+CD28+ T cells in young and elderly persons but a similarity between CD8+CD28- T cells in young and elderly persons. As CD8+CD28+ T cells from elderly and young persons are distinct due to a different composition of the population, these results suggest that the gene expression profile does not depend on chronological age but depends on the differentiation state of the individual cell types.
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Separate analysis of CD28+ and CD28− CD8+ T cells in young and old individuals was consistent with old CD28+CD8+ T cells being more differentiated, as also suggested by epigenetic studies.
“A separate analysis of CD28+ and CD28− CD8+ T cells in young and old individuals were consistent with the model that old CD28+CD8+ T cells were more differentiated (Lazuardi et al., 2009) as also suggested by the epigenetic studies of CD8+ T cell subsets (Moskowitz et al., 2017).”
From Successful and Maladaptive T Cell Aging (2017) · cites this paper as Lazuardi et al., 2009
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References 31 · Cited by 43
- Authors
- Lazuardi, L., Herndler-Brandstetter, D., Brunner, S., Laschober, G. T., Lepperdinger, G., & Grubeck-Loebenstein, B.
- Affiliations
- Austrian Academy of Sciences (AT)
- Venue
- Biogerontology
- Published
- 2008-08-27
- Volume
- 10
- Issue
- 2
- Pages
- 191-202
- ISSN
- 1389-5729 (ISSN-L)
- 1573-6768
- OpenAlex
- W2082872592
- Cited by
- 43
- References
- 31
- Citations by year
- 2026: 1 · 2025: 1 · 2023: 1 · 2022: 3 · 2021: 1 · 2019: 2 · 2018: 1 · 2017: 3 · 2016: 1 · 2015: 3 · 2014: 2 · 2013: 2 · 2012: 5
- FWCI
- 2.36
- Type
- journal article
- Language
- en
- Open access
- green
- epub.oeaw.ac.at
- Licence
- other-oa
- www.springer.com/tdm (tdm)
- Locations
- Biogerontology (publishedVersion)
- PubMed (publishedVersion)
- Oesterreichisches Musiklexikon online (Institut für kunst- und musikhistorische Forschungen der Österreichischen Akademie der Wissenschaften) (submittedVersion, other-oa)
- Austrian Biographical Dictionary (Austrian Academy of Sciences) (submittedVersion, other-oa)
- Funders
- Austrian Science Fund
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
- Keywords
- CD8+CD28- T cells, T cell gene expression, aging, age-related gene expression, T cell differentiation, gene expression profiling
- MeSH terms
- Adult, Age Factors, Aged, Aged, 80 and over, Aging, Cell Differentiation, Female, Humans, Immunologic Memory, Male, Reproducibility of Results, Cluster Analysis, T-Lymphocyte Subsets, CD28 Antigens, CD8-Positive T-Lymphocytes, Reverse Transcriptase Polymerase Chain Reaction, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling
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