MiR-338-3p inhibits TNF-α-induced lipogenesis in human sebocytes.
Abstract
Objective To suppress TNF-α-induced lipogenesis in sebocytes (associated with acne development) with microRNA-338-3p (miR-338-3p) and to explore the underlying mechanisms.Results TNF-α increased lipid droplet formation in sebocytes which were used as in vitro model of inflammation-induced acne. Flow cytometry and TLC assays validated that miR-338-3p could suppress TNF-α-induced lipid droplet formation, down-regulate the expression of PREX2a, and inactivate AKT signaling in sebocytes. In addition, suppression of AKT activity by the PI3 K and AKT inhibitors diminished TNF-α-induced lipogenesis. PREX2a siRNA mimics the effects of miR-338-3p on AKT phosphorylation and lipogenesis. PREX2a overexpression consistently restored lipogenesis and AKT phosphorylation attenuated by miR-338-3p.Conclusions MiR-338-3p suppresses the TNF-α-induced lipogenesis in sebocytes by targeting PREX2a and down-regulating PI3K/AKT signaling.
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Activating miR-338, to some extent, can influence the emotional function of the brain.
“Thus, activating miR-338, to some extent, can influence the emotional function of the brain [38].”
Silencing miR-338-5p can result in neuronal polarity loss and a considerable decrease in the number of neurons.
“Other researches have shown that silencing miR-338-5p can result in neuronal polarity loss and a considerable decrease in the number of neurons.”
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References 11 · Cited by 20
- Authors
- Liu, J., Cao, L., Feng, Y., Li, Y., & Li, T.
- Affiliations
- Northeast Forestry University (CN)
- Corresponding author
- Li, Tao
- Venue
- Biotechnology Letters
- Published
- 2017-06-09
- Volume
- 39
- Issue
- 9
- Pages
- 1343-1349
- ISSN
- 0141-5492 (ISSN-L)
- 1573-6776
- OpenAlex
- W2623384553
- Cited by
- 20
- References
- 11
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 1 · 2022: 4 · 2021: 3 · 2020: 5 · 2019: 1 · 2018: 1
- FWCI
- 2.17
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biotechnology Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Heilongjiang province scientific research institution innovation grants (YC2016D002)
- Topics
- Health Sciences › Medicine › Dermatology › Acne and Rosacea Treatments and Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- miR-338-3p, TNF-α, sebocytes, lipogenesis, lipid droplet formation, acne, PI3K/Akt signaling, Akt phosphorylation, microRNA
- MeSH terms
- Cells, Cultured, Chromatography, Thin Layer, Epithelial Cells, Flow Cytometry, Humans, Tumor Necrosis Factor-alpha, Signal Transduction, Phosphatidylinositol 3-Kinases, Guanine Nucleotide Exchange Factors, MicroRNAs, Lipogenesis, Proto-Oncogene Proteins c-akt, Lipid Droplets
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