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Cited by 272

The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.

High levels of microRNA-21 in the stroma of colorectal cancers predict short disease-free survival in stage II colon cancer patients

Open access
Nielsen, Boye Schnack, Jørgensen, Stine, Fog, Jacob Ulrik, Søkilde, Rolf, Christensen, Ib Jarle, Hansen, Ulla, Brünner, Nils, Baker, Adam, Møller, Søren, Nielsen, Hans Jørgen·2011·Clinical & Experimental Metastasis
DOI 10.1007/s10585-010-9355-7
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Abstract

Approximately 25% of all patients with stage II colorectal cancer will experience recurrent disease and subsequently die within 5 years. MicroRNA-21 (miR-21) is upregulated in several cancer types and has been associated with survival in colon cancer. In the present study we developed a robust in situ hybridization assay using high-affinity Locked Nucleic Acid (LNA) probes that specifically detect miR-21 in formalin-fixed paraffin embedded (FFPE) tissue samples. The expression of miR-21 was analyzed by in situ hybridization on 130 stage II colon and 67 stage II rectal cancer specimens. The miR-21 signal was revealed as a blue chromogenic reaction, predominantly observed in fibroblast-like cells located in the stromal compartment of the tumors. The expression levels were measured using image analysis. The miR-21 signal was determined as the total blue area (TB), or the area fraction relative to the nuclear density (TBR) obtained using a red nuclear stain. High TBR (and TB) estimates of miR-21 expression correlated significantly with shorter disease-free survival (p = 0.004, HR = 1.28, 95% CI: 1.06-1.55) in the stage II colon cancer patient group, whereas no significant correlation with disease-free survival was observed in the stage II rectal cancer group. In multivariate analysis both TB and TBR estimates were independent of other clinical parameters (age, gender, total leukocyte count, K-RAS mutational status and MSI). We conclude that miR-21 is primarily a stromal microRNA, which when measured by image analysis identifies a subgroup of stage II colon cancer patients with short disease-free survival.

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Metadata

Authors
Nielsen, B. S., Jørgensen, S., Fog, J. U., Søkilde, R., Christensen, I. J., Hansen, U., Brünner, N., Baker, A., Møller, S., & Nielsen, H. J.
Venue
Clinical & Experimental Metastasis
Publisher
Springer Science and Business Media LLC
Published
2010-10-31
Volume
28
Issue
1
Pages
27-38
ISSN
  • 0262-0898 (ISSN-L)
  • 1573-7276
DOI
10.1007/s10585-010-9355-7
OpenAlex
W2015423537
Cited by
272
References
51
FWCI
6.14
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: High levels of microRNA-21 in the stroma of colorectal cancers predict short disease-free survival in stage II colon cancer patients. https://app.cochat.ai/papers/10.1007/s10585-010-9355-7
The paper
Nielsen, B. S., Jørgensen, S., Fog, J. U., Søkilde, R., Christensen, I. J., Hansen, U., Brünner, N., Baker, A., Møller, S., & Nielsen, H. J. (2011). High levels of microRNA-21 in the stroma of colorectal cancers predict short disease-free survival in stage II colon cancer patients. Clinical & Experimental Metastasis. https://doi.org/10.1007/s10585-010-9355-7

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