The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Optimization of a microplate reader method for the analysis of changes in mitochondrial membrane potential in Chlamydomonas reinhardtii cells using the fluorochrome JC-1
Open accessAbstract
The fluorochrome JC-1 is mainly used in mammalian cells to estimate mitochondrial membrane potential (MMP) as a stress marker, with far less data being available for plants or green algae. To address this, we have validated the possibility for changes in MMP to be used as a sensitive stress indicator in the green alga Chlamydomonas reinhardtii . To optimize the method, we analyzed the conditions for applicable MMP determination, including the proper buffer, excitation/emission wavelengths, and solvent (dimethyl sulfoxide, DMSO) influence on the fluorescence signal, and evaluated usefulness of the method for toxicological research. Our results have allowed us to develop a complete protocol for the estimation of MMP changes in C. reinhardtii . Statistical analyses have confirmed the reproducibility, sensitivity, and applicability of this method for toxicological studies.
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“Dyes such as SYTOX Green or propidium iodide allow differentiation between dead and living cells, while the JC-1 probe facilitates the assessment of mitochondrial activity, which is valuable in studies investigating the effects of environmental stress on microalgae [234].”
From Multi-Sensing Monitoring of the Microalgae Biomass Cultivation Systems for Biofuels and Added Value Products Synthesis—Challenges and Opportunities (2025) · cites this paper as [234]
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MITOTIC REPLICATION OF DEOXYRIBONUCLEIC ACID IN CHLAMYDOMONAS REINHARDI
Sueoka, N. (1960) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.46.1.83 · Cited by 791
[29] Mitochondrial membrane potential monitored by JC-1 dye
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Cytometric analysis of immunosenescence
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Self-citation
Research works printed but not linked: 1
- Harris EH (2009) The Chlamydomonas sourcebook: introduction to Chlamydomonas and its laboratory use. Academic Press, New York
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Dębowski, M., Kazimierowicz, J., & Zieliński, M. (2025) · Applied Sciences · doi:10.3390/app15137324 · Cited by 30
Metadata
- Authors
- Harshkova, D., Zielińska, E., & Aksmann, A.
- Affiliations
- University of Gdańsk (PL)
- Corresponding author
- Aksmann, Anna
- Venue
- Journal of Applied Phycology
- Published
- 2019-07-10
- Volume
- 31
- Issue
- 6
- Pages
- 3691-3697
- ISSN
- 0921-8971 (ISSN-L)
- 1573-5176
- OpenAlex
- W2960489995
- Cited by
- 16
- References
- 31
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 5 · 2023: 1 · 2022: 2 · 2021: 1 · 2020: 3
- FWCI
- 0.50
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Journal of Applied Phycology (publishedVersion, cc-by)
- Funders
- National Science Centre Poland (UMO-2016/23/B/NZ9/00963)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Keywords
- mitochondrial membrane potential, Chlamydomonas reinhardtii, JC-1, microplate reader, green algae, stress symptoms, dimethyl sulfoxide, toxicology, method validation
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- CoChat. (2026). Citation check: Optimization of a microplate reader method for the analysis of changes in mitochondrial membrane potential in Chlamydomonas reinhardtii cells using the fluorochrome JC-1. https://app.cochat.ai/papers/10.1007/s10811-019-01860-3
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- Harshkova, D., Zielińska, E., & Aksmann, A. (2019). Optimization of a microplate reader method for the analysis of changes in mitochondrial membrane potential in Chlamydomonas reinhardtii cells using the fluorochrome JC-1. Journal of Applied Phycology. https://doi.org/10.1007/s10811-019-01860-3
