Novel association between apoptotic sperm biomarkers with seminal biochemical parameters and acetylcholinesterase activity in patients with teratozoospermia
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Objective We aimed to determine whether the dysfunction of physiological apoptosis and specific seminal biochemical parameters could be associated with male infertility and sperm morphological defects. Study design Ejaculated sperm samples from sixty patients with isolated teratozoospermia and thirty fertile donors were analyzed. The proportion of both viable and dead spermatozoa expressing activated caspases was detected by fluorescence microscopy through the use of different specific carboxyfluorescein-labeled caspase inhibitors FLICA. The different stages of apoptosis in human were qualitatively and quantitatively determined by using the AO/EB fluorescent staining method. The levels of the seminal biochemical parameters (acetylcholinesterase (AChE), lactate dehydrogenase (LDH), creatine phosphokinase (CK), iron (Fe), calcium (Ca), and phosphorus (P)) were evaluated spectrophotometrically. Results Patients with teratozoospermia showed significantly higher proportions of dead and live spermatozoa with activated caspases and spermatozoa in the late stage of apoptosis when compared to controls. Among the different studied biochemical seminal parameters, the rates of acetylcholinesterase activity, creatine phosphokinase, iron, and calcium were significantly increased in the patient group. However, the rate of phosphorus was significantly decreased. Interestingly, significant relationships were found between the studied biochemical and apoptotic biomarkers and the rates of atypi…
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One study found that patients with teratozoospermia had a higher proportion of spermatozoa with late-stage apoptosis, and there was a substantial correlation between the frequencies of atypical sperm forms and apoptotic biomarkers.
“One study found that patients with teratozoospermia had a higher proportion of spermatozoa with late-stage apoptosis, and there was a substantial correlation between the frequencies of atypical sperm forms and apoptotic biomarkers (81).”
From Isolated teratozoospermia: revisiting its relevance in male infertility: a narrative review (2024) · cites this paper as [81]
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- Authors
- Ammar, O., Mehdi, M., Tekeya, O., Neffati, F., & Haouas, Z.
- Affiliations
- University of Monastir (TN)
- Hospital Fatuma Bourguiba Monastir (TN)
- Corresponding author
- Ammar, Oumaima
- Venue
- Journal of Assisted Reproduction and Genetics
- Published
- 2019-09-11
- Volume
- 36
- Issue
- 11
- Pages
- 2367-2378
- ISSN
- 0740-7769 (ISSN-L)
- 1058-0468
- 1573-7330
- PMC
- PMC6885487
- OpenAlex
- W2973033983
- Cited by
- 11
- References
- 80
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 3 · 2022: 1 · 2021: 1 · 2020: 2
- FWCI
- 0.96
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Assisted Reproduction and Genetics (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministère de l’Enseignement Supérieur, de la Recherche Scientifique et des Technologies de l'Information et de la Communication (This work was supported by funds allocated to the Research laboratory of Histology and Genetic LR40ES18 by the Ministère Tunisien de l’Enseingement Supérieur et de la Recherché Scientifique.)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Reproductive biology and impacts on aquatic species
- Keywords
- teratozoospermia, sperm apoptosis, acetylcholinesterase, seminal plasma biochemistry, caspase activation, male infertility, creatine kinase, lactate dehydrogenase, iron, calcium, phosphorus
- MeSH terms
- Teratozoospermia, Acetylcholinesterase, Adult, Fertility, Humans, Infertility, Male, Male, Prospective Studies, Semen, Sperm Motility, Spermatozoa, Biomarkers, Apoptosis
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