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Prevention the formation of biofilm on orthopedic implants by melittin thin layer on chitosan/bioactive glass/vancomycin coatings
Open accessAbstract
Methicillin-resistant and Vancomycin-resistant Staphylococcus aureus bacteria (MRSA and VRSA, respectively) can seriously jeopardizes bone implants. This research aimed to examine the potential synergistic effects of Melittin and vancomycin in preventing MRSA and VRSA associated bone implant infections. Chitosan/bioactive glass nanoparticles/vancomycin composites were coated on hydrothermally etched titanium substrates by casting method. The composite coatings were coated by Melittin through drop casting technique. Melittin raised the proliferation of MC3T3 cells, making it an appropriate option as osteoinductive and antibacterial substance in coatings of orthopedic implants. Composite coatings having combined vancomycin and Melittin eliminated both planktonic and adherent MRSA and VRSA bacteria, whereas coatings containing one of them failed to kill the whole VRSA bacteria. Therefore, chitosan/bioactive glass/vancomycin/Melittin coating can be used as a bone implant coating because of its anti-infective properties.
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Peptide-based therapies have a dual ability to combat infection and promote healing.
“Peptide-based therapies, which encompass antimicrobial peptides and growth factors, are particularly appealing for their dual ability to combat infection and promote healing [23,24].”
From Advancements in Regenerative Therapies for Orthopedics: A Comprehensive Review of Platelet-Rich Plasma, Mesenchymal Stem Cells, Peptide Therapies, and Biomimetic Applications (2025) · cites this paper as [23]
The Abstract reports that melittin, an antimicrobial peptide, increased MC3T3 cell proliferation and that coatings combining melittin with vancomycin eliminated MRSA and VRSA bacteria. This supports infection control and a healing-related cellular effect in this specific implant coating, but the paper does not establish that peptide-based therapies generally, including growth factors, both combat infection and promote healing. (LLM, conf=0.86)
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Research works printed but not linked: 25
- Raphel J, Holodniy M, Goodman SB, Heilshorn SC. Multifunctional coatings to simultaneously promote osseointegration and prevent infection of orthopaedic implants. Biomaterials. 2016. https://doi.org/10.1016/j.biomaterials.2016.01.016. · DOI
- Goodman SB, Yao Z, Keeney M, Yang F. The future of biologic coatings for orthopaedic implants. Biomaterials. 2013. https://doi.org/10.1016/j.biomaterials.2013.01.074. · DOI
- Kazemzadeh-Narbat M, Lai BF, Ding C, Kizhakkedathu JN, Hancock RE, Wang R. Multilayered coating on titanium for controlled release of antimicrobial peptides for the prevention of implant-associated infections. Biomaterials. 2013. https://doi.org/10.1016/j.biomaterials.2013.04.036. · DOI
- Pashaei F, Bevalian P, Akbari R, Bagheri KP. Single dose eradication of extensively drug resistant Acinetobacter spp. in a mouse model of burn infection by melittin antimicrobial peptide. Microb Pathogen. 2019. https://doi.org/10.1016/j.micpath.2018.11.055. · DOI
- Andersson DI. Persistence of antibiotic resistant bacteria. Curr Opin Microbiol. 2003. https://doi.org/10.1016/j.mib.2003.09.001. · DOI
- Ionita D, Bajenaru-Georgescu D, Totea G, Mazare A, Schmuki P, Demetrescu I. Activity of vancomycin release from bioinspired coatings of hydroxyapatite or TiO2 nanotubes. Inter J Pharm. 2017. https://doi.org/10.1016/j.ijpharm.2016.11.062. · DOI
- Hiramatsu K. Vancomycin-resistant Staphylococcus aureus: a new model of antibiotic resistance. Lancet Infect Dis. 2001. https://doi.org/10.1016/S1473-3099(01)00091-3. · DOI
- Walsh TR, Howe RA. The prevalence and mechanisms of vancomycin resistance in Staphylococcus aureus. Ann Rev Microb. 2002. https://doi.org/10.1146/annurev.micro.56.012302.160806. · DOI
- Weigel LM, Clewell DB, Gill SR, Clark NC, McDougal LK, Flannagan SE, et al. Genetic analysis of a high-level vancomycin-resistant isolate of Staphylococcus aureus. Science. 2003. https://doi.org/10.1126/science.1090956. · DOI
- Friaes A, Resina C, Manuel V, Lito L, Ramirez M, Melo-Cristino J. Epidemiological survey of the first case of vancomycin-resistant Staphylococcus aureus infection in Europe. Epid Infect. 2015. https://doi.org/10.1017/S0950268814001423. · DOI
- Peter M, Binulal NS, Soumya S, Nair SV, Furuike T, Tamura H, et al. Nanocomposite scaffolds of bioactive glass ceramic nanoparticles disseminated chitosan matrix for tissue engineering applications. Carbohydr Polym. 2010. https://doi.org/10.1016/j.carbpol.2009.08.001. · DOI
- Boccaccini AR, Erol M, Stark WJ, Mohn D, Hong Z, Mano JF. Polymer/bioactive glass nanocomposites for biomedical applications: a review. Composit Sci Technol. 2010. https://doi.org/10.1016/j.compscitech.2010.06.002. · DOI
- Arul KT, Ramesh M, Chennakesavan C, Karthikeyan V, Manikandan E, Umar A et al. Novel multifunctional of magnesium ions (Mg+ +) incorporated calcium phosphate nanostructures. J Alloys Compd. 2018. https://doi.org/10.1016/j.jallcom.2017.09.254. · DOI
- Kolanthai E, Sindu PA, Arul KT, Chandra VS, Manikandan E, Kalkura SN. Agarose encapsulated mesoporous carbonated hydroxyapatite nanocomposites powder for drug delivery. J Photochem Photobiol B: Biol. 2017. https://doi.org/10.1016/j.jphotobiol.2016.12.005. · DOI
- Ramadoss P, Arul KT, Ramya JR, Begam MR, Chandra VS, Manikandan E. Enhanced mechanical strength and sustained drug release of gelatin/keratin scaffolds. Mater Lett. 2017. https://doi.org/10.1016/j.matlet.2016.09.095. · DOI
- Yang CC, Lin CC, Yen SK. Electrochemical deposition of vancomycin/chitosan composite on Ti alloy. J Electrochem Soc. 2011 https://doi.org/10.1149/2.105112jes. · DOI
- Gebhardt F, Seuss S, Turhan MC, Hornberger H, Virtanen S, Boccaccini AR. Characterization of electrophoretic chitosan coatings on stainless steel. Mater Lett. 2012. https://doi.org/10.1016/j.matlet.2011.08.088. · DOI
- Mehdipour M, Afshar A, Mohebali M. Electrophoretic deposition of bioactive glass coating on 316L stainless steel and electrochemical behavior study. Appl Surf Sci. 2012. https://doi.org/10.1016/j.apsusc.2012.06.038. · DOI
- Ordikhani F, Simchi A. Long-term antibiotic delivery by chitosan-based composite coatings with bone regenerative potential. Appl Surf Sci. 2014. https://doi.org/10.1016/j.apsusc.2014.07.197. · DOI
- Cruz J, Kawasaki M, Gorski W. Electrode coatings based on chitosan scaffolds. Anal Chem. 2000. https://doi.org/10.1021/ac990954b. · DOI
- Haimi S, Moimas L, Pirhonen E, Lindroos B, Huhtala H, Räty S, et al. Calcium phosphate surface treatment of bioactive glass causes a delay in early osteogenic differentiation of adipose stem cells. J Biomed Mater Res A. 2009. https://doi.org/10.1002/jbm.a.32233. · DOI
- Brogden KA. Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria?. Nature Rev Microbiol. 2005. https://doi.org/10.1038/nrmicro1098. · DOI · Paper page
- Yarlagadda V, Samaddar S, Paramanandham K, Shome BR, Haldar J. Membrane disruption and enhanced inhibition of cell‐wall biosynthesis: a synergistic approach to tackle vancomycin‐resistant bacteria. Angew Chem Int Ed Engl. 2015. https://doi.org/10.1002/anie.201507567. · DOI
- Xie W, Xu P, Wang W, Liu Q. Preparation and antibacterial activity of a water-soluble chitosan derivative. Carbohydr Polym. 2002. https://doi.org/10.1016/S0144-8617(01)00370-8. · DOI
- Echezarreta-Lopez MM, Landin M. Using machine learning for improving knowledge on antibacterial effect of bioactive glass. Inter J Pharm. 2013. https://doi.org/10.1016/j.ijpharm.2013.06.036. · DOI
Cited by
Sorted by citations
Goulian, A. J., Goldstein, B., & Saad, M. A. (2025) · Journal of Clinical Medicine · doi:10.3390/jcm14062061 · Cited by 25
“Peptide-based therapies, which encompass antimicrobial peptides and growth factors, are particularly appealing for their dual ability to combat infection and promote healing [23,24].”
Metadata
- Authors
- Zarghami, V., Ghorbani, M., Bagheri, K. P., & Shokrgozar, M. A.
- Affiliations
- Sharif University of Technology (IR)
- Pasteur Institute of Iran (IR)
- Corresponding author
- Ghorbani, Mohammad
- Bagheri, Kamran Pooshang
- Venue
- Journal of Materials Science: Materials in Medicine
- Published
- 2021-06-22
- Volume
- 32
- Issue
- 7
- Pages
- 75-75
- ISSN
- 0957-4530 (ISSN-L)
- 1573-4838
- PMC
- PMC8219550
- OpenAlex
- W3173906486
- Cited by
- 26
- References
- 0
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 7 · 2023: 4 · 2022: 6 · 2021: 3
- FWCI
- 4.70
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Journal of Materials Science Materials in Medicine (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Lirias (KU Leuven) (submittedVersion, cc-by)
- Topics
- Health Sciences › Medicine › Surgery › Orthopedic Infections and Treatments
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Engineering › Mechanical Engineering › Surface Treatment and Coatings
- Keywords
- melittin, vancomycin, biofilm inhibition, orthopedic implants, methicillin-resistant Staphylococcus aureus, vancomycin-resistant Staphylococcus aureus, chitosan, bioactive glass nanoparticles, composite coatings, osteoinduction, MC3T3-E1 cells, antibacterial coatings, synergistic antibacterial activity
- MeSH terms
- Antimicrobial Peptides, Animals, Anti-Bacterial Agents, Bacterial Adhesion, Ceramics, Melitten, Microscopy, Electron, Scanning, Orthopedics, Powders, Staphylococcus aureus, Surface Properties, Titanium, Vancomycin, 3T3 Cells, Biofilms, Bone Substitutes, Prostheses and Implants, Coated Materials, Biocompatible, Vancomycin Resistance, Chitosan, Cell Proliferation, Mice, Nanoparticles, Methicillin-Resistant Staphylococcus aureus
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