Effect of molecular chaperone on the soluble expression of recombinant fab fragment in E. coli
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Coexpression of DnaK/DnaJ/GrpE results in a 2.5-fold increase in the periplasmic expression level of an anti-TNF-α Fab in shake flasks using LB medium and BL21(DE3) strains.
“For instance, Dariushnejad and coworkers have reported that the coexpression of DnaK/DnaJ/GrpE (DnaKJE) results in a 2.5-fold increase in the periplasmic expression level of an anti-TNF-α Fab in shake flasks, using LB medium and BL21(DE3) strains [99,100].”
From Evolution of Escherichia coli Expression System in Producing Antibody Recombinant Fragments (2020) · cites this paper as [100]
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Evolution of Escherichia coli Expression System in Producing Antibody Recombinant Fragments
Sandomenico, A., Sivaccumar, J. P., & Ruvo, M. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21176324 · Cited by 134 · see citing works
“5-fold increase in the periplasmic expression level of an anti-TNF-α Fab in shake flasks, using LB medium and BL21(DE3) strains [99,100].”
Method
- Authors
- Farajnia, S., Ghorbanzadeh, V., & Dariushnejad, H.
- Affiliations
- Tabriz University of Medical Sciences (IR)
- Lorestan University of Medical Sciences (IR)
- Corresponding author
- Dariushnejad, Hassan
- Venue
- International Journal of Peptide Research and Therapeutics
- Published
- 2019-03-09
- Volume
- 26
- Issue
- 1
- Pages
- 251-258
- ISSN
- 1573-3149 (ISSN-L)
- 1573-3904
- OpenAlex
- W2920843420
- Cited by
- 20
- References
- 0
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 1 · 2023: 3 · 2022: 6 · 2021: 2 · 2020: 3
- FWCI
- 0.91
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- International Journal of Peptide Research and Therapeutics (publishedVersion)
- Funders
- Drug Applied Research Center, Tabriz University of Medical Sciences (94/54)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
- Keywords
- molecular chaperones, Fab fragment, anti-TNF-alpha antibody, soluble protein expression, protein solubility, DnaK, Hsp40, GroEL, thioredoxin, TNF-α, recombinant antibodies, Escherichia coli expression
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