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GM130-silencing may aggravate blood-brain barrier damage and affect microglia polarization by down-regulating PD-L1 expression after experimental intracerebral hemorrhage
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Abstract
Background In addition to primary injury, secondary injuries related to BBB disruption and immune-inflammatory response also play an important role in intracerebral hemorrhage (ICH). And the Golgi apparatus play an important role in the state of ICH. Methods ICH model and GM130-silencing ICH model were established in SD rats. The Garcia score was used to score the neurological defects of the rats. Blood-brain barrier (BBB) integrity were assessed by amount of extravasated Evans blue, and tight junction proteins. The expression of PD-L1 and GM130were detected through Western-blot and the subtype of microglia was showing with Immunofluorescence staining. Results Compared with the ICH group, GM130-silencing ICH rats got a worsened neurological deficit and enlarged volume of the hematoma. Evan's blue extravasation aggravated as well. The expression of GM130 in peri-hematoma tissue was further decreased, and the morphology and structure of the Golgi apparatus were further damaged. Meanwhile, the GM130 deficit resulted in decreased expression of PD-L1 and more polarization of microglia to the M1 subtype. Conclusion We demonstrate that GM130 could influence the integrity of BBB and plays a role in neuroinflammation via regulation of PD-L1 after ICH. The manipulation of GM130 might be a promising therapeutical target in ICH.
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Metadata
- Authors
- Chen, X., Ren, Y., Xie, P., Lei, Q., & Lu, W.
- Affiliations
- Central South University (CN)
- Changsha Central Hospital (CN)
- Corresponding author
- Lu, Wei
- Venue
- Molecular Biology Reports
- Published
- 2024-08-19
- Volume
- 51
- Issue
- 1
- Pages
- 919-919
- ISSN
- 0301-4851 (ISSN-L)
- 1573-4978
- OpenAlex
- W4401701148
- Cited by
- 5
- References
- 1
- Citations by year
- 2026: 4 · 2025: 1
- FWCI
- 1.34
- Type
- journal article
- Language
- en
- Open access
- green
- dx.doi.org
- Licence
- Locations
- Molecular Biology Reports (publishedVersion)
- Research Square (acceptedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- This work was funded by the National Natural Science Foundation of China (#81571181)
- Topics
- Health Sciences › Medicine › Clinical Neurology › Intracerebral and Subarachnoid Hemorrhage Research
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Health Sciences › Medicine › Clinical Neurology › Traumatic Brain Injury and Neurovascular Disturbances
- Keywords
- GM130, Golgi apparatus, intracerebral hemorrhage, blood-brain barrier disruption, PD-L1, microglial polarization, M1 microglia, neuroinflammation, secondary brain injury, tight junction proteins, hematoma volume, neurological deficit
- MeSH terms
- Animals, Autoantigens, Blood-Brain Barrier, Cerebral Hemorrhage, Disease Models, Animal, Golgi Apparatus, Male, Membrane Proteins, Down-Regulation, Rats, Sprague-Dawley, Microglia, Rats, B7-H1 Antigen
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- Chen, X., Ren, Y., Xie, P., Lei, Q., & Lu, W. (2024). GM130-silencing may aggravate blood-brain barrier damage and affect microglia polarization by down-regulating PD-L1 expression after experimental intracerebral hemorrhage. Molecular Biology Reports. https://doi.org/10.1007/s11033-024-09859-x
