Molecular Mechanisms of Resistance to 5-Fluorocytosine in Laboratory Mutants of Candida glabrata
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Resistance to 5-fluorocytosine (5-FC) has been poorly investigated in the yeast Candida glabrata. This study was conducted on laboratory mutants obtained by exposure of a wild-type isolate to 5-FC. Based on their susceptibility to 5-fluorouracil (5-FU), two of these mutants were selected for further analysis of the molecular mechanisms of 5-FC resistance. One mutant, resistant to both compounds, exhibited a missense mutation in the gene coding the cytosine deaminase and a decrease in the expression level of the gene coding the uridine monophosphate pyrophosphorylase. The other mutant that showed a reduced susceptibility to 5-FC and 5-FU exhibited an overexpression of the genes coding the thymidylate synthase and a cytosine permease, associated with a missense mutation in the last gene. Thus, beside mutations in the FUR1 gene which represent the most common cause of resistance to 5-FC, other mechanisms may also occur in C. glabrata.
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Decreased susceptibility towards flucytosine has been associated with point mutations in FCY1, FCY2, and FUR1 genes and the deletion of FPS1 and FPS2 genes.
“Decreased susceptibility towards flucytosine has been associated with point mutations in FCY1, FCY2, and FUR1 genes and the deletion of FPS1 and FPS2 genes [36,114,115,116,117].”
From Evolutionary Emergence of Drug Resistance in Candida Opportunistic Pathogens (2018) · cites this paper as [116]
Such flucytosine resistance mechanisms have been observed in C. albicans, C. lusitaniae, and C. glabrata.
“Such resistance mechanisms have been observed in C. albicans [114], C. lusitaniae [115], and C. glabrata [116,117].”
From Evolutionary Emergence of Drug Resistance in Candida Opportunistic Pathogens (2018) · cites this paper as [116]
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References 35 · Cited by 55
- Authors
- Vandeputte, P., Pineau, L., Larcher, G., Noel, T., Brèthes, D., Chabasse, D., & Bouchara, J.-P.
- Affiliations
- Centre Hospitalier Universitaire d'Angers (FR)
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Vandeputte, Patrick
- Venue
- Mycopathologia
- Published
- 2010-07-09
- Volume
- 171
- Issue
- 1
- Pages
- 11-21
- ISSN
- 0301-486X (ISSN-L)
- 1573-0832
- OpenAlex
- W1990206664
- Cited by
- 55
- References
- 35
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 5 · 2023: 3 · 2022: 2 · 2021: 4 · 2020: 2 · 2019: 7 · 2018: 6 · 2017: 7 · 2016: 4 · 2014: 1 · 2013: 1 · 2012: 6
- FWCI
- 1.86
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- other-oa
- www.springer.com/tdm (tdm)
- Locations
- Mycopathologia (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- LillOA (Université de Lille (University Of Lille)) (submittedVersion, other-oa)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Fungal Plant Pathogen Control
- Keywords
- Candida glabrata, 5-fluorocytosine resistance, antifungal resistance, cytosine deaminase, thymidylate synthase, missense mutations, gene overexpression
- MeSH terms
- Antifungal Agents, DNA Mutational Analysis, Flucytosine, Fluorouracil, Pentosyltransferases, Thymidylate Synthase, Gene Expression, Amino Acid Substitution, Mutation, Missense, Drug Resistance, Fungal, Candida glabrata, Cytosine Deaminase
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