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Cited by 20Nail Diseases and TreatmentsAntifungal resistance and susceptibilityFungal Infections and Studies

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In Vitro Activity of Antifungal Drugs Against Trichophyton rubrum and Trichophyton mentagrophytes spp. by E-Test Method and Non-supplemented Mueller–Hinton Agar Plates

Intra, Jari, Sarto, Cecilia, Mazzola, Selene, Fania, Chiara, Tiberti, Natalia, Brambilla, Paolo·2019·Mycopathologia
DOI 10.1007/s11046-019-00360-9
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Abstract

Trichophyton rubrum and Trichophyton mentagrophytes spp. are two of the most frequently isolated dermatophytes causing dermatophytosis worldwide. Since the incidence of resistance to antifungal agents is increasing, antifungal susceptibility tests are needed to successfully treat dermatophytoses. Most of the methods currently available are complicated, time-consuming and lack of reference procedures. The aim of this work was to establish a simple protocol to test the susceptibility of dermatophytes isolated from clinical samples against five antifungal drugs using E-test and disk diffusion methods. We used the E-test on non-supplemented Mueller-Hinton agar plates to determine the minimum inhibitory concentrations (MICs) of fluconazole, itraconazole, voriconazole and amphotericin B, and disk diffusion method to determine the interpretive MIC of terbinafine. Fifty dermatophytes-10 T. rubrum and 40 T. mentagrophytes spp.-were assessed after only 96 h of colony growth. Terbinafine was the most active antifungal agent with an inhibition diameter greater than 70 mm (sensitivity > 20 mm), followed by voriconazole, itraconazole and amphotericin B with MICs ranging from 0.032 to 0.38 µg/mL, from 0.006 to 0.125 µg/mL and from 0.5 to 1.5 µg/mL, respectively. All isolates were resistant to fluconazole. Collectively, the less laborious E-test and disk diffusion method were shown to be suitable and reliable to determine antifungal sensitivity of dermatophytes. This simple standard protocol…

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  • The agar and broth dilution tests can be time-consuming because they require preparation of a series of agar plates or broth tubes each having a different concentration of the antimicrobial agent.

    “The agar and broth dilution tests can be time-consuming because they require the preparation of a series of agar plates or broth tubes each having a different concentration of the antimicrobial agent [53].”

    From Methods for screening and evaluation of antimicrobial activity: A review of protocols, advantages, and limitations (2024) · cites this paper as [53]

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Metadata

Authors
Intra, J., Sarto, C., Mazzola, S., Fania, C., Tiberti, N., & Brambilla, P.
Affiliations
  • University of Milano-Bicocca (IT)
  • Ospedale Sacro Cuore Don Calabria (IT)
Corresponding author
Intra, Jari
ORCID
Venue
Mycopathologia
Publisher
Springer Science and Business Media LLC
Published
2019-07-11
Volume
184
Issue
4
Pages
517-523
ISSN
  • 0301-486X (ISSN-L)
  • 1573-0832
DOI
10.1007/s11046-019-00360-9
OpenAlex
W2956877842
Cited by
20
References
0
Citations by year
2026: 1 · 2025: 3 · 2024: 6 · 2023: 3 · 2022: 2 · 2021: 3 · 2020: 2
FWCI
1.33
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Mycopathologia (publishedVersion)
  • PubMed (publishedVersion)
  • BOA (University of Milano-Bicocca) (submittedVersion)
Topics
  • Health Sciences › Medicine › Dermatology › Nail Diseases and Treatments
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Health Sciences › Medicine › Infectious Diseases › Fungal Infections and Studies
Keywords
Trichophyton rubrum, Trichophyton mentagrophytes, dermatophytes, dermatophytosis, antifungal susceptibility testing, minimum inhibitory concentration, E-test, disk diffusion, fluconazole resistance, terbinafine, voriconazole, itraconazole, amphotericin B
MeSH terms
Agar, Antifungal Agents, Culture Media, Humans, Microbial Sensitivity Tests, Tinea, Trichophyton

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