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Cited by 47Spaceflight effects on biologyFlavonoids in Medical ResearchPhytochemicals and Antioxidant Activities

Protective Effects of Flavonoids Against Oxidative Stress Induced by Simulated Microgravity in SH-SY5Y Cells

Qu, Lina, Chen, Hailong, Liu, Xinmin, Bi, Lei, Xiong, Jianghui, Mao, Zebin, Li, Yinghui·2010·Neurochemical Research
DOI 10.1007/s11064-010-0205-4
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Abstract

Many lines of evidence suggest that microgravity results in increased oxidative stress in the nervous system. In order to protect neuronal cells from oxidative damage induced by microgravity, we selected some flavonoids that might prevent oxidative stress because of their antioxidant activities. Among the 20 flavonoids we examined, we found that isorhamnetin and luteolin had the best protective effects against H(2)O(2) or SIN-1-induced cytotoxicity in SH-SY5Y cells. Using a clinostat to simulate microgravity, we found that isorhamnetin and luteolin treatment protected SH-SY5Y cells by preventing microgravity-induced increases in reactive oxygen species (ROS), nitric oxide (NO) and 3-nitrotyrosine (3-NT) levels, and a decrease in antioxidant power (AP). Moreover, isorhamnetin and luteolin treatment downregulated the expression of inducible nitric oxide synthase (iNOS), and oxidative stress was significantly inhibited by an iNOS inhibitor in SH-SY5Y cells exposed to simulated microgravity (SMG). These results indicate that isorhamnetin and luteolin could protect against microgravity-induced oxidative stress in neuroblastoma SH-SY5Y cells by inhibiting the ROS-NO pathway. These two flavonoids may have potential for preventing oxidative stress induced by space flight or microgravity.

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Authors
Qu, L., Chen, H., Liu, X., Bi, L., Xiong, J., Mao, Z., & Li, Y.
Affiliations
  • Peking University (CN)
  • China Astronaut Research and Training Center (CN)
  • Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
Corresponding author
  • Mao, Zebin
  • Li, Yinghui
ORCID
Venue
Neurochemical Research
Publisher
Springer Science and Business Media LLC
Published
2010-06-23
Volume
35
Issue
9
Pages
1445-1454
ISSN
  • 0364-3190 (ISSN-L)
  • 1573-6903
DOI
10.1007/s11064-010-0205-4
OpenAlex
W2020851500
Cited by
47
References
40
Citations by year
2026: 3 · 2025: 3 · 2024: 7 · 2023: 2 · 2022: 1 · 2021: 7 · 2020: 1 · 2019: 4 · 2018: 1 · 2017: 5 · 2016: 5 · 2015: 1 · 2014: 1 · 2013: 3 · 2012: 2
FWCI
1.30
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Neurochemical Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Academy of Medical Sciences
  • National Natural Science Foundation of China
  • Chinese Academy of Sciences
  • Chinese Academy of Medical Sciences
Topics
  • Health Sciences › Medicine › Physiology (medical) › Spaceflight effects on biology
  • Health Sciences › Medicine › Pharmacology (medical) › Flavonoids in Medical Research
  • Health Sciences › Medicine › Biochemistry (medical) › Phytochemicals and Antioxidant Activities
Keywords
flavonoids, microgravity, oxidative stress, isorhamnetin, luteolin, SH-SY5Y cells, reactive oxygen species, nitric oxide, inducible nitric oxide synthase, nitrotyrosine, antioxidant activity, hydrogen peroxide, SIN-1, neuroprotection, spaceflight
MeSH terms
Animals, Antioxidants, Cell Survival, Humans, Hydrogen Peroxide, Molsidomine, Neurons, Nitric Oxide, Quercetin, Tyrosine, Weightlessness, Molecular Structure, Oxidants, Reactive Oxygen Species, Oxidative Stress, Nitric Oxide Donors, Flavonols, Cell Line, Tumor, Luteolin, Nitric Oxide Synthase Type II

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