The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Characterization of an Arabidopsis thaliana mutant lacking a cytosolic non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase
Open accessAbstract
Non-phosphorylating glyceraldehyde- 3-phosphate dehydrogenase (NP-GAPDH) is a conserved cytosolic protein found in higher plants. In photosynthetic cells, the enzyme is involved in a shuttle transfer mechanism to export NADPH from the chloroplast to the cytosol. To investigate the role of this enzyme in plant tissues, we characterized a mutant from Arabidopsis thaliana having an insertion at the NP-GAPDH gene locus. The homozygous mutant was determined to be null respect to NP-GAPDH, as it exhibited undetectable levels of both transcription of NP-GAPDH mRNA, protein expression and enzyme activity. Transcriptome analysis demonstrated that the insertion mutant plant shows altered expression of several enzymes involved in carbohydrate metabolism. Significantly, cytosolic phosphorylating (NAD-dependent) glyceraldehyde-3-phosphate dehydrogenase mRNA levels are induced in the mutant, which correlates with an increase in enzyme activity. mRNA levels and enzymatic activity of glucose-6-phosphate dehydrogenase were also elevated, correlating with an increase in NADPH concentration. Moreover, increased ROS levels were measured in the mutant plants. Down-regulation of several glycolytic and photosynthetic genes suggests that NP-GAPDH is important for the efficiency of both metabolic processes. The results presented demonstrate that NP-GAPDH has a relevant role in plant growth and development.
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In the citing paper
“Consistent with this, Arabidopsis lines with decreased cytosolic GAPDH are slow growing and have decreased levels of ATP in illuminated leaves (Rius et al., 2006).”
From Leaf Energy Balance Requires Mitochondrial Respiration and Export of Chloroplast NADPH in the Light (2019) · cites this paper as Rius et al., 2006
In the citing paper
“These lines also showed decreased leaf respiration, which is not consistent with our prediction.”
From Leaf Energy Balance Requires Mitochondrial Respiration and Export of Chloroplast NADPH in the Light (2019) · cites this paper as Rius et al., 2006
In the citing paper
“Similarly, data for the maximal catalytic activities of phosphorylating NAD-GAPDH (93 μmol m−2 s−1; Gibon et al., 2004) and nonphosphorylating NADP-GAPDH (0.33 μmol m−2 s−1; Rius et al., 2006) in Arabidopsis leaves were used to constrain the upper limit of the chloroplast TP-3PGA shuttle.”
From Leaf Energy Balance Requires Mitochondrial Respiration and Export of Chloroplast NADPH in the Light (2019) · cites this paper as Rius et al., 2006
In the citing paper
“Moreover, the maximum rate of the reaction from experimental data (0.33 μmol m−2 s−1) means that it cannot have a major bearing on the overall exchange of energy and reducing power between compartments in this system.”
From Leaf Energy Balance Requires Mitochondrial Respiration and Export of Chloroplast NADPH in the Light (2019) · cites this paper as Rius et al., 2006
Citations
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Cited by
Redox Regulation in Photosynthetic Organisms: Signaling, Acclimation, and Practical Implications
Foyer, C. H., & Noctor, G. (2008) · Antioxidants and Redox Signaling · doi:10.1089/ars.2008.2177 · Cited by 1,474
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The metabolomics of oxidative stress
Noctor, G., Lelarge‐Trouverie, C., & Mhamdi, A. (2014) · Phytochemistry · doi:10.1016/j.phytochem.2014.09.002 · Cited by 265
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Metadata
- Authors
- Rius, S. P., Casati, P., Iglesias, A. A., & Gomez-Casati, D. F.
- Affiliations
- Consejo Nacional de Investigaciones Científicas y Técnicas (AR)
- National University of Rosario (AR)
- Venue
- Plant Molecular Biology
- Published
- 2006-08
- Volume
- 61
- Issue
- 6
- Pages
- 945-957
- ISSN
- 0167-4412 (ISSN-L)
- 1573-5028
- OpenAlex
- W2075280488
- Cited by
- 94
- References
- 49
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 5 · 2023: 5 · 2022: 2 · 2021: 8 · 2020: 7 · 2019: 5 · 2018: 6 · 2017: 6 · 2016: 5 · 2015: 6 · 2014: 5 · 2013: 6 · 2012: 4
- FWCI
- 1.22
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- cc-by-nc-sa
- www.springer.com/tdm (tdm)
- Locations
- Plant Molecular Biology (publishedVersion)
- PubMed (publishedVersion)
- CONICET Digital (CONICET) (submittedVersion, cc-by-nc-sa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Keywords
- Arabidopsis thaliana mutants, insertion mutants, carbohydrate metabolism, glucose-6-phosphate dehydrogenase, reactive oxygen species, glycolysis, photosynthesis, plant growth and development
- MeSH terms
- Carbon Dioxide, Chlorophyll, Cytosol, Glycolysis, Microscopy, Fluorescence, Mutation, NADP, Phenotype, Photosynthesis, RNA, Messenger, Superoxides, Gene Expression Regulation, Enzymologic, Mutagenesis, Insertional, Arabidopsis, Reactive Oxygen Species, Gene Expression Regulation, Plant, Gene Expression Regulation, Developmental, Plant Leaves, Reverse Transcriptase Polymerase Chain Reaction, Oligonucleotide Array Sequence Analysis, Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating), Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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