CoChat
Sign Up
Cited by 205Essential Oils and Antimicrobial ActivityFungal Plant Pathogen ControlInsect Pest Control Strategies

The DOI matches its Crossref record.

Fungitoxicity of the essential oil of Citrus sinensis on post-harvest pathogens

Sharma, Neeta, Tripathi, Abhishek·2006·World Journal of Microbiology and Biotechnology
DOI 10.1007/s11274-005-9075-3
Open this paper in CoChat — free account

Abstract

The essential oil extracted from the epicarp of Citrus sinensis exhibited absolute fungitoxicity against the 10 post-harvest pathogens. GC-MS studies of the oil revealed the presence of 10 chemical constituents, of which limonene was found to be the major component (84.2%). The activity of the oil was tested by the poisoned food technique (PF) and the volatile activity (VA) assay and the oils showed greater toxicity in the VA assay than in the poisoned food assay. The nature of the toxicity was studied in the VA assay and it was observed that the oil was fungicidal for the 10 pathogens in the 700 ppm (mg/l) to 1000 ppm range. The oil was extremely toxic for spore germination and it was found that at 700 ppm, spore germination was inhibited in the 10 test fungi out of the 12 tested. Treatment at 300 ppm concentration exhibited 70-100% inhibition of spore germination in most of the fungi tested. Scanning electron microscopy (SEM) was done to study the mode of action of the oil in Aspergillus niger and it was observed that treatment with the oil leads to distortion and thinning of the hyphal wall and the reduction in hyphal diameter and absence of conidiophores.

Overview

Integrity signals

Registry

Crossref · 10.1007/s11274-005-9075-3

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 40

  1. Adams R.P., 1995. Identification of Essential Oil Components by Gas Chromatography/Mass Spectroscopy Allured Publishing Corporation Carol Stream, Illinois ISBN 0931710421
  2. Antonov A., Stewart A., Walter M., 1997. Inhibition of conidium germination and mycelial growth of Botrytis cineria by natural productsProceeding 50th New Zealand Plant Protection Conference 159–164 · DOI
  3. Baruah P., Sharma R.K., Singh R.S., Ghosh A.C., 1996. Fungicidal activity of some naturally occurring essential oils against Fusarium moniliformeJournal of Essential Oil Research 8: 411–441 · DOI
  4. Beg A.Z., Ahmad I., 2002. In vitro fungitoxicity of the essential oil of Syzygium aromaticum World Journal of Microbiology and Biotechnology 18: 313–315
  5. Begum J., Yusuf M., Chowdhary J.U., Wahab M.A., 1993. Studies on essential oils for their antibacterial and antifungal properties. Part I. preliminary screening of 35 essential oilsBangladesh Journal of Science and Industrial Research 28: 25–34
  6. Bishop C.D., Reagan J., 1998. Control of storage pathogen Botrytis cinerea on Dutch white cabbage (Brassica oleracea var. capitata) by essential oils of Monarda citriodora var. citriodora and Melaleuca alternifolia on the post harvest pathogens Journal of Essential Oil Research 10: 57–60 · DOI
  7. Bishop C.D., Thornton I.B., 1997. Evaluation of the antifungal activity of the essential oil of Monarda citriodora var. citriodora and Melaleuca alternifolia on post harvest pathogensJournal of Essential Oil Research 9: 77–82 · DOI
  8. de Billerbeck V.G., Roques C.G., Bessiere J.M. Fonvieille J.L., Dargent R., 2001. Effects of Cymbopogon nardus (L.) W. Watson essential oil on the growth and morphogenesis of Aspergillus niger Canadian Journal of Microbiology 47: 9–17 · DOI
  9. Delespaul D., de Billerbeck V.G., Roques C.G., Michel G., Marquier-Vinuales C., Bessiere J.M., 2000. The antifungal activity of essential oils as determined by different screening methods Journal of Essential Oil Research 12: 256–266 · DOI
  10. Dianz F., Santos M., Blanco R., Tello J.C., 2002. Fungicides resistance in Botrytis cinerea isolate from strawberry crops in Huelva (southwestern Spain) Phytoparasitica 30: 529–534 · DOI
  11. Dixit S.N., Chandra H., Tiwari R., Dixit V., 1995. Development of botanical fungicides against blue mold of mandarinsJournal of Stored Product Research 31: 165–172 · DOI
  12. Dubey, N.K. & Kishore, N. 1988 Exploitation of higher plant products as natural fumigants. In Proceedings of Fifth International Congress on Plant Pathology, Kyoto, Japan, pp. 423 (Abstract)
  13. Ernestina A., Miguel Á.M., Maria M.H., Socorro V., Eduardo P., Irasema V., 2003. Fungicidal potential of methoxylated flavones from citrus for in vitro control of Colletotrichum gloeosporioides, causal agent of anthracnose disease in tropical fruits Pest Management Science 59: 1245–1249 · DOI
  14. Grover R.K., Moore J.D., 1962. Toxicometric studies of fungicides against brown rot organisms Sclerotinia fructicola and S. laxaPhytopathology 52: 876–880
  15. Guenther E., 1948. The Essential Oils 1 D. Van Nostrand Co, New York, NY Princeton York Allured Publication Carol Stream, IL pp 774
  16. Hidalgo P.J., Ubera J.L., Santos J.A., LaFront F., Castelanos C., Palomino A., Roman M., 2002. Essential oils in Culamintha sylvatica Bromf. Ssp. Ascendens (Jorden) P.W. Ball wild and cultivated productions and antifungal activityJournal of Essential Oil Research 14: 68–71 · DOI
  17. Jain N.K., 1977. Antifungal activity of essential oil of Aegle marmelos Correa (Rutaceae)Indian Drugs and Pharmaceutical Industry 12: 55
  18. Karapinar M., 1985. The effects of citrus oils and some spices on growth and aflatoxin production by Aspergillus parasiticus NRRL 2999International Journal of Food Microbiology 2: 239–245 · DOI
  19. Lingk W., 1991. Health risk evaluation of pesticides contaminations in drinking water Gesunde Pflanzen 43: 21–25
  20. Meepagala K.M., Sturtz G., Wedge D.E., 2002. Antifungal constituents of the essential oil fraction of Artemisia drancunculus L. var. dracunculusJournal of Agricultural and Food Chemistry 50: 6989–6992 · DOI
  21. Moss M.O., 2002. Mycotoxin review. 1. Aspergillus and PenicilliumMycologist 16: 116–119
  22. Nguefack J., Leth V., AmvamZollo P.H., Mathur S.B. 2004. Evaluation of five essential oils from aromatic plants of Cameroon for controlling food spoilage and mycotoxin producing fungiInternational Journal of Food Microbiology 94: 329–334 · DOI
  23. Nychas G.J.E., 1995. Natural antimicrobials from plants In: Gould G.W., (Ed). New Methods of Food Preservation Academic Professional London. Blackie (58 pp) ISBN 0751400483 · DOI
  24. Pandey D.K., Tripathi N.N., Tripathi R.D., Dixit S.N., 1982. Fungitoxic and phytotoxic properties of the essential oil of H. suaveolensZeitschrift für Pflanzenkrankheiten und Pflanzenschutz 89: 344–349
  25. Pattnaik S., Subramanyam V.R., Kole C., 1996. Antibacterial and antifungal activities of ten essential oils in vitroMicrobios 86: 237–246
  26. Phillips D.J., 1984. Mycotoxins as a postharvest problem In: Moline H.E., (Ed.), Postharvest Pathology of Fruits and Vegetables: Postharvest Losses in Perishable Crops Agricultural Experimental Station, University of California, Berkeley publications NE pp. 50–54
  27. Reimann S., Deising H.B., 2000. Fungicides: risk of resistance development and search for new targetsArchives of Phytopathology and Plant Protection 33: 329–349 · DOI
  28. Reuveni R., Fleischer A., Putievski E., 1984. Fungistatic activity of essential oils from Ocimum basilicum chemotypesPhytopathology 10: 20–22 · DOI
  29. Sharma N., 1998. Control of post-harvest diseases with natural plant products. In Neeta Sharma, Alam M.M., Ed. Postharvest Diseases of Horticultural Perishables International Book Distributing Company Lucknow 226 004 (India) pp. 1–27 ISBN 81-85860-21-1
  30. Sharma, N. & Verma, U.K. 2004 Bioactivity of Hyptis suaveolens on storage mycoflora. In International Conference on Fumigation and Controlled Atmosphere, pp. 55. Conrad Jupiters, Gold coast, Australia (Abstract)
  31. Shukla A.C., Shahi S.K., Dixit A., 2000. Epicarp of Citrus sinensis: a potential source of natural pesticidesIndian Phytopathology 53: 468–471
  32. Singh A.K., Dikshit A., Sharma M.L., Dixit S.N., 1980. Fungitoxic activity of some essential oilsEconomic Botany 34: 186–190 · DOI
  33. Singh G., Upadhyay R.K., Narayanan C.S., Padmkumari K.P., Rao G.P., 1993. Chemical and fungitoxic investigations on the essential oil of Citrus sinensis (L.) Pers Journal of Plant Diseases and Protection 100: 69–74
  34. Soliman K.M., Badeaa R.I., 2002. Effect of oil extracted from some medicinal plants on different mycotoxigenic fungiFood and Chemical Technology 40: 1669–1675 · DOI
  35. Surender P., Janalah C., Reddy V.K., Reddy S.M., 1987. Antifungal activity of secretions of scent glands from Heteropteram bugsIndian Journal of Experimental Biology 25: 233–234
  36. Thomson D.P., 1989. Fungitoxic activity of essential oil components on food storage fungiMycologia 81: 151–153 · DOI
  37. Tiwari R., Mishra D.N., Upadhyay P.S., 1988. Efficacy of some plant volatiles for the control of black mould of onion caused by Aspergillus niger Van Tiegh during storageNational Academy Science Letters 11: 345–347
  38. Unnikrishnan V., Nath B.S., 2002. Hazardous chemicals in foodsIndian Journal of Dairy Bioscience 11: 155–158
  39. Wilson C.L., Wisniewski M.E., 1989. Biological control of postharvest diseases of fruits and vegetables: an emerging technologyAnnual Review of Phytopathology 27: 425–441 · DOI
  40. Zambonelli A., Zechini d’ Aulerio A., Bianchi A., Albasini A. 1996. Effects of essential oil on phytopathogenic fungiPhytopathology 144: 491–494 · DOI

Cited by

Sorted by citations

Metadata

Authors
Sharma, N., & Tripathi, A.
Affiliations
University of Lucknow (IN)
Corresponding author
Sharma, Neeta
Venue
World Journal of Microbiology and Biotechnology
Publisher
Springer Science and Business Media LLC
Published
2006-01-24
Volume
22
Issue
6
Pages
587-593
ISSN
  • 0959-3993 (ISSN-L)
  • 1573-0972
DOI
10.1007/s11274-005-9075-3
OpenAlex
W1972773936
Cited by
205
References
0
Citations by year
2026: 3 · 2025: 12 · 2024: 9 · 2023: 8 · 2022: 19 · 2021: 13 · 2020: 10 · 2019: 12 · 2018: 10 · 2017: 12 · 2016: 9 · 2015: 11 · 2014: 13 · 2013: 19 · 2012: 13
FWCI
8.57
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
World Journal of Microbiology and Biotechnology (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Fungal Plant Pathogen Control
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Insect Pest Control Strategies
Keywords
Citrus sinensis, essential oils, fungitoxicity, limonene, spore germination inhibition, poisoned food technique, Aspergillus niger

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review