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Cited by 79Yeasts and Rust Fungi StudiesFungal Plant Pathogen ControlPostharvest Quality and Shelf Life Management

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Screening and identification of epiphytic yeasts with potential for biological control of green mold of citrus fruits

Taqarort, Naima, Echairi, Abdelouahed, Chaussod, Remi, Nouaim, Rachida, Boubaker, Hassan, Benaoumar, Abdellah A., Boudyach, Elhassan·2008·World Journal of Microbiology and Biotechnology
DOI 10.1007/s11274-008-9849-5
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Abstract

Epiphytic yeasts isolated from the surface of citrus fruits, harvested in several orchards in the Souss-Massa-Drâa Valley, Agadir, Morocco, were in vivo screened for antagonistic activity against Penicillium digitatum, the causal agent of green mold of citrus. From a total of 245 yeast strains assessed for their biocontrol activity against P. digitatum, fifteen reduced the incidence of disease to less than 50%. The effectiveness of the best selected yeast strains showed that Pichia anomala (YT73), Debaryomyces hansenii (YT22) and Hanseniaspora guilliermondii (YT13) were the most effective, with a reduction of green mold incidence from 65 to ~80%, compared to the control. The identification of the fifteen selected yeast strains was carried out through an integrated approach including phenotypic and genotypic (sequencing of D1/D2 domain of 26S rDNA encoding gene) methods. These 15 selected were identified as: H. guilliermondii, D. hansenii, H. uvarum and P. anomala. The study of the dynamics of two of the best strains, H. guilliermondii and D. hansenii, showed that these strains can grow rapidly, by approximately 2 log units, in citrus fruit wounds. Such rapid growth in wounds indicates that these antagonist yeasts are excellent colonizers of citrus wounds and can thrive on citrus fruits as a substrate.

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Metadata

Authors
Taqarort, N., Echairi, A., Chaussod, R., Nouaim, R., Boubaker, H., Benaoumar, A. A., & Boudyach, E.
Affiliations
  • Université Ibn Zohr (MA)
  • Institut National de la Recherche Agronomique (MA)
Corresponding author
Boubaker, Hassan
ORCID
Venue
World Journal of Microbiology and Biotechnology
Publisher
Springer Science and Business Media LLC
Published
2008-09-11
Volume
24
Issue
12
Pages
3031-3038
ISSN
  • 0959-3993 (ISSN-L)
  • 1573-0972
DOI
10.1007/s11274-008-9849-5
OpenAlex
W1997184408
Cited by
79
References
1
Citations by year
2026: 1 · 2025: 2 · 2024: 6 · 2023: 3 · 2022: 5 · 2021: 6 · 2020: 6 · 2019: 5 · 2018: 6 · 2017: 5 · 2016: 5 · 2014: 5 · 2013: 5 · 2012: 5
FWCI
1.91
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
World Journal of Microbiology and Biotechnology (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Microbiology › Yeasts and Rust Fungi Studies
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Fungal Plant Pathogen Control
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Postharvest Quality and Shelf Life Management
Keywords
epiphytic yeasts, biological control, Penicillium digitatum, citrus fruit, Wickerhamomyces anomalus, Debaryomyces hansenii, Hanseniaspora guilliermondii, Hanseniaspora uvarum, antagonistic activity, yeast strain identification, 26S rDNA D1/D2 domain, wound colonization

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CoChat. (2026). Citation check: Screening and identification of epiphytic yeasts with potential for biological control of green mold of citrus fruits. https://app.cochat.ai/papers/10.1007/s11274-008-9849-5
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Taqarort, N., Echairi, A., Chaussod, R., Nouaim, R., Boubaker, H., Benaoumar, A. A., & Boudyach, E. (2008). Screening and identification of epiphytic yeasts with potential for biological control of green mold of citrus fruits. World Journal of Microbiology and Biotechnology. https://doi.org/10.1007/s11274-008-9849-5