CoChat
Sign Up
Cited by 46Agriculture, Plant Science, Crop ManagementPlant-Microbe Interactions and ImmunityPhosphorus and nutrient management

The DOI matches its Crossref record.

Solubilization of insoluble inorganic phosphates by a novel temperature-, pH-, and salt-tolerant yeast, Rhodotorula sp. PS4, isolated from seabuckthorn rhizosphere, growing in cold desert of Ladakh, India

Mundra, Sunil, Arora, Richa, Stobdan, Tsering·2011·World Journal of Microbiology and Biotechnology
DOI 10.1007/s11274-011-0708-4
Open this paper in CoChat — free account

Abstract

The study was aimed to develop biofertilizer solubilizing inorganic phosphates for region experiencing temperature, pH and salt stressed conditions. A yeast strain PS4, which was temperature-, pH- and salt-tolerant and capable of solubilizing insoluble inorganic phosphate was isolated from rhizosphere of seabuckthorn (Hippophae rhamnoides L.), growing in the Indian Trans-Himalaya. Based on morphological, biochemical, whole cell FAME analysis and molecular characterization, strain PS4 was identified as Rhodotorula sp. The soluble phosphate production under optimal conditions at pH 7 and 30°C was 278.3 mg l−1. Strain PS4 showed ability to solubilize insoluble phosphate under different stress conditions viz. 5–40°C temperature, 1–5% salt concentration and 3–11 pH range. Soluble phosphate production from Ca3(PO4)2 under combined stress conditions at extreme values of temperature, pH and salt concentration showed 81.6–83.2% reduction as compare to optimal conditions after 5 days incubation. The strain solubilize Ca3(PO4)2 to a great extent than FePO4 and AlPO4. The solubilization of insoluble phosphate was associated with drop in pH of the culture media. Inoculation of tomato seedling with the strain increased fruit yield, roots and shoot length. Rhodotorula sp. PS4 with phosphate-solubilizing ability under stress conditions appeared to be attractive for exploring their plant growth-promoting activity towards the development of microbial inoculants in stressed region.

Overview

Integrity signals

Registry

Crossref · 10.1007/s11274-011-0708-4

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 55

  1. Arun AB, Sridhar KR (2005) Growth tolerance of Rhizobia isolated from sand dune legumes of the Southwest coast of India. Eng Life Sci 5:134–138 · DOI
  2. Bai FY, Cai Y, Wang QM, Ohkubo H (2004) Rhodotorula oryzae sp. nov., a novel basidiomycetous yeast species isolated from paddy rice. Antonie van Leeuwenhoek 86:295–299 · DOI
  3. Belimov AA, Postavskaya SM, Khamova OF, Kozehmyakov AP, Kunakova AM, Gruzdeva EV (1994) Effectiveness of barley inoculation with root diazotrophic bacteria and their survival depending on soil moisture and temperature. Microbiology 63:506–510
  4. Brady NC (1990) The nature and properties of soils. Macmillan, New York
  5. Chen YP, Rekha PD, Arun AB, Shen FT, Lai WA, Young CC (2006) Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Appl Soil Ecol 34:33–41 · DOI
  6. Douka CE, Apostalakis CG, Skarloy VD (1978) Studies of Rhizobium meliloti isolated from salt-affected soil. Ann Appl Biol 88:457–460 · DOI
  7. Fasim F, Ahmed N, Parson R, Gadd GM (2002) Solubilization of zinc salts by a bacterium isolated from air environment of a tannery. FEMS Microbiol Lett 213:1–6 · DOI
  8. Fell JW, Statzell-Tallman A (1998) Rhodotorula F.C. Harrison. In: Kurtzman CP, Fell JW (eds) The yeasts, a taxonomic study, 4th edn. Elsevier, Amsterdam, pp 800–827
  9. Fell JW, Boekhout T, Fonseca A, Scorzetti G, Statzell-Tallman A (2000) Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50:1351–1371 · DOI
  10. Fernandez LA, Zalba P, Gomez MA, Sagardoy MA (2007) Phosphate-solubilization activity of bacterial strains in soil and their effect on soybean growth under greenhouse conditions. Biol Fertil Soils 43:805–809 · DOI
  11. Gadanho M, Sampaio JP (2002) Polyphasic taxonomy of the basidiomycetous yeast genus Rhodotorula: Rh. glutinis sensu stricto and Rh. dairenensis comb. nov. FEMS Yeast Res 2:47–58
  12. Gaind S, Gaur AC (1991) Thermotolerant phosphate solubilizing microorganisms and their interaction with mung-bean. Plant Soil 133:141–149 · DOI
  13. Goldstein AH (1986) Bacterial solubilization of mineral phosphates: historical perspectives and future prospects. Am J Altern Agric 1:51–57 · DOI
  14. Goldstein AH, Braverman K, Osorio N (1999) Evidence for mutualism between a plant growing in a phosphate-limited desert environment and a mineral phosphate solubilizing (MPS) bacterium. FEMS Microbiol Ecol 3:295–300 · DOI
  15. Gulati A, Rahi P, Vyas P (2008) Characterization of phosphate-solubilizing fluorescent pseudomonads from the rhizosphere of seabuckthorn growing in the cold deserts of Himalayas. Curr Microbiol 56:73–79 · DOI
  16. Gulati A, Vyas P, Kasana RC (2009) Plant growth-promoting and rhizosphere-competent Acinetobacter rhizosphaerae Strain BIHB 723 from the cold deserts of the Himalayas. Curr Microbiol 58:371–377 · DOI
  17. Gyaneshwar P, Parekh LJ, Archana G, Poole PS, Collins MD, Hutson RA, Kumar GN (1999) Involvement of a phosphate starvation inducible glucose dehydrogenase in soil phosphate solubilization by Enterobacter asburiae. FEMS Microbiol Lett 171:223–229 · DOI
  18. Hamamoto M, Nakase T (2000) Phylogenetic analysis of the ballistoconidium-forming yeast genus Sporobolomyces based on 18S rDNA sequences. Int J Syst Evol Microbiol 50:1373–1380 · DOI
  19. Hilda R, Fraga R (1999) Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol Adv 17:319–339 · DOI
  20. Hua SST, Tsai V, Lichens GM, Noma AT (1982) Accumulation of amino acids in Rhizobium spp WR 10001 in response to sodium chloride salinity. Appl Environ Microbiol 44:135–140 · DOI
  21. Illmer P, Schinner F (1995) Solubilization of inorganic calcium phosphates-solubilization mechanisms. Soil Biol Biochem 27:257–263 · DOI · Paper page
  22. Johri JK, Surange S, Nautiyal CS (1999) Occurrence of salt, pH, and temperature-tolerant, phosphate-solubilizing bacteria in alkaline soils. Curr Microbiol 39:89–93 · DOI
  23. Jones DL, Darrah PR (1994) Role of root derived organic acids in the mobilization of nutrients from the rhizosphere. Plant Soil 166:247–257 · DOI
  24. Komagata K, Nakase T (1967) Reitoshokuhin no biseibutsu nikansuru kenkyu V Shihan reitoshokuhin yori bunri shita kobo no seijo (microbiological studies on frozen foods). Shokuhin Eiseigaku Zasshi 8:53–57 · DOI
  25. Kulkarni S, Nautiyal CS (2000) Effects of salt and pH stress on temperature-tolerant Rhizobium sp. NBRI1330 nodulating Prosopis juliflora. Curr Microbiol 40:221–226 · DOI
  26. Kumar GP, Murkute AA, Gupta S, Singh SB (2009) Carbon sequestration with special reference to agroforestry in cold deserts of Ladakh. Curr Sci 97:1063–1068
  27. Leyval C, Berthelin J (1989) Interaction between Laccaria laccata, Agrobacterium radiobacter and beech roots: influence on P, K, Mg and Fe mobilization from mineral and plant growth. Plant Soil 117:103–110 · DOI
  28. Mnasri B, Mrabet M, Laguerre G, Aouani M, Mhamdi R (2007) Salt-tolerant rhizobia isolated from a Tunisian oasis that are highly effective for symbiotic N2-fixation with Phaseolus vulgaris constitute a novel biovar (bv. Mediterranense) of Sinorhizobium meliloti. Arch Microbiol 187:79–85 · DOI
  29. Murphy J, Riley JP (1962) A modified single solution method for the determination of phosphate in natural waters. Anal Chim Acta 27:31–36 · DOI
  30. Nagahama T, Hamamoto M, Horikoshi K (2006) Rhodotorula pacifica sp. nov., a novel yeast species from sediment collected on the deep-sea floor of the North-west Pacific Ocean. Int J Syst Evol Microbiol 56:295–299 · DOI
  31. Nakase T, Suzuki M (1986) Bullera megalospora, a new species of yeast forming large ballistospores isolated from dead leaves of Oryza sativa, Miscanthus sinensis and Sasa sp. in Japan. J Gen Appl Microbiol 32:225–240 · DOI
  32. Nautiyal CS, Bhadauria S, Kumar P, Lal H, Verma MD (2000) Stress induced phosphate solubilization in bacteria isolated from alkaline soils. FEMS Microbiol Lett 182:291–296 · DOI · Paper page
  33. Oehl F, Oberson A, Probst M, Fliessbach A, Roth HR, Frossard E (2001) Kinetics of microbial phosphorus uptake in cultivated soils. Biol Fertil Soil 34:31–41 · DOI
  34. Omar SA (1998) The role of rock-phosphate-solubilizing fungi and vesicular–arbuscular–mycorrhiza (VAM) in growth of wheat plants fertilized with rock phosphate. World J Microbiol Biotechnol 14:211–218 · DOI
  35. Orhan E, Esitken A, Ercisli S, Turan M, Sahin F (2006) Effect of plant growth promoting rhizobacteria (PGPR) on yield, growth and nutrient contents in organically grown raspberry. Sci Hortic 111:38–43 · DOI
  36. Pal SS (1998) Interactions of an acid tolerant strain of phosphate solubilizing bacteria with a few acid tolerant crops. Plant Soil 198:169–177 · DOI
  37. Pikovskaya RI (1948) Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Mikrobiologiya 17:362–373
  38. Puente ME, Bashan Y, Li CY, Lebsky VK (2004) Microbial populations and activities in the rhizoplane of rock-weathering desert plants. Root colonization and weathering of igneous rocks. Plant Biol 6:629–642 · DOI
  39. Rangarajan S, Saleena LM, Nair S (2002) Diversity of Pseudomonas spp. isolated from rice rhizosphere populations grown along a salinity gradient. Microb Ecol 43:280–289 · DOI
  40. Rehman A, Nautiyal CS (2002) Effect of drought on the growth and survival of the stress-tolerant bacterium Rhizobium sp. NBRI2505 sesbania and its drought-sensitive transposon n5 mutant. Curr Microbiol 45:368–377 · DOI
  41. Rodriguez H, Gonzalez T, Selman G (2000) Expression of a mineral phosphate solubilizing gene from Erwinia herbicola in two rhizobacterial strains. J Biotechnol 84:155–161 · DOI
  42. Rodriguez H, Fraga R, Gonzalez T, Bashan Y (2006) Genetics of phosphate solubilization and its potential applications for improving plant growth promoting bacteria. Plant Soil 287:15–21 · DOI
  43. Sasser M (1990a) Technical note 102. Tracking a strain using the microbial identification system. MIS, Newark
  44. Sasser M (1990b) Identification of bacteria through fatty acid analysis. In: Klement Z, Rudolph K, Sands D (eds) Methods in phytobacteriology. Akademiai Kiado, Budapest, pp 199–204
  45. Sasser M, Wichman MD (1991) Identification of microorganisms through use of gas chromatography and high-performance liquid chromatography. In: Balows A, Hausler WJ, Herrman KL, Isenberg HD, Shadomy HJ (eds) Manual of clinical microbiology, 5th edn. American Society for Microbiology, Washington
  46. Selvakumar G, Kundu S, Joshi P, Nazim S, Gupta AD, Mishra PK, Gupta HS (2008) Characterization of a cold-tolerant plant growth-promoting bacterium Pantoea dispersa 1A isolated from a sub-alpine soil in the North Western Indian Himalayas. World J Microbiol Biotechnol 24:955–960 · DOI
  47. Sharan A, Shikha, Daramwal NS, Gaur R (2008) Xanthomonas campestris, a novel stress tolerant, phosphate-solubilizing bacterial strain from saline-alkali soils. World J Microbiol Biotechnol 24:753–759 · DOI
  48. Shivaji S, Bhadra B, Rao RS, Pradhan S (2008) Rhodotorula himalayensis sp. nov., a novel psychrophilic yeast isolated from Roopkund Lake of the Himalayan mountain ranges, India. Extremophiles 12:375–381 · DOI
  49. Son HJ, Park GT, Cha MS, Heo MS (2006) Solubilization of insoluble inorganic phosphates by a novel salt- and pH-tolerant Pantoea agglomerans R-42 isolated from soybean rhizosphere. Bioresour Technol 97:204–210 · DOI
  50. Tripura C, Sashidhar B, Podile AR (2007) Ethyl methanesulfonate mutagenesis-enhanced mineral phosphate solubilization by groundnut-associated Serratia marcescens GPS-5. Curr Microbiol 54:79–84 · DOI
  51. Vyas P, Rahi P, Chauhan A, Gulati A (2007) Phosphate solubilizing potential and stress tolerance of Eupenicillium parvum from tea soil. Mycol Res 111:931–938 · DOI
  52. White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M, Gelfand D, Sninsky JJ, White TJ (eds) PCR protocols. Academic Press, San Diego, pp 315–322 · DOI · Paper page
  53. Yarrow D (1998) Methods for the isolation, maintenance and identification of yeasts. In: Kurtzman CP, Fell JW (eds) The yeasts, a taxonomic study, 4th edn. Elsevier, Amsterdam, pp 77–100 · DOI
  54. Young CC, Chang CH, Chen LF, Chao CC (1998) Characterization of the nitrogen fixing and ferric phosphate solubilizing bacteria isolated from Taiwan soil. J Chin Agric Chem Soc 36:201–210
  55. Zahran HH (1999) Rhizobium legume symbiosis and nitrogen fixation under severe conditions and in arid climate. Microbiol Mol Biol Rev 63:968–989 · DOI

Cited by

Sorted by citations

See all 46

Metadata

Authors
Mundra, S., Arora, R., & Stobdan, T.
Affiliations
Defence Institute of High Altitude Research (IN)
Corresponding author
Stobdan, Tsering
ORCID
Venue
World Journal of Microbiology and Biotechnology
Publisher
Springer Science and Business Media LLC
Published
2011-03-08
Volume
27
Issue
10
Pages
2387-2396
ISSN
  • 0959-3993 (ISSN-L)
  • 1573-0972
DOI
10.1007/s11274-011-0708-4
OpenAlex
W2087660294
Cited by
46
References
0
Citations by year
2026: 5 · 2025: 1 · 2024: 3 · 2023: 2 · 2022: 4 · 2021: 8 · 2020: 2 · 2019: 3 · 2018: 2 · 2017: 4 · 2016: 3 · 2014: 3 · 2013: 3 · 2012: 3
FWCI
3.47
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
World Journal of Microbiology and Biotechnology (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Agriculture, Plant Science, Crop Management
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
  • Physical Sciences › Environmental Science › Waste Management and Disposal › Phosphorus and nutrient management
Keywords
phosphate solubilization, Rhodotorula, yeast, inorganic phosphate, calcium phosphate, salt tolerance, pH tolerance, heat tolerance, biofertilizers, plant growth-promoting microorganisms, tomato, cold desert, Ladakh, microbial inoculants

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Solubilization of insoluble inorganic phosphates by a novel temperature-, pH-, and salt-tolerant yeast, Rhodotorula sp. PS4, isolated from seabuckthorn rhizosphere, growing in cold desert of Ladakh, India. https://app.cochat.ai/papers/10.1007/s11274-011-0708-4
The paper
Mundra, S., Arora, R., & Stobdan, T. (2011). Solubilization of insoluble inorganic phosphates by a novel temperature-, pH-, and salt-tolerant yeast, Rhodotorula sp. PS4, isolated from seabuckthorn rhizosphere, growing in cold desert of Ladakh, India. World Journal of Microbiology and Biotechnology. https://doi.org/10.1007/s11274-011-0708-4