Mitogenic response and probiotic characteristics of lactic acid bacteria isolated from indigenously pickled vegetables and fermented beverages
Abstract
Lactic acid bacteria from indigenous pickled vegetables and fermented beverages (fermented rice and Madhuca longifolia flowers) were isolated and investigated for their functional characteristics in vitro as potential new probiotic strains. Four isolates (all Lactobacillus spp.) selected on the basis of high tolerance to bile (0.2%) were identified by standard and molecular methods (16S rDNA) as L. helveticus, L. casei, L. delbrueckii and L. bulgaricus from pickled vegetables and fermented beverages respectively. These selected strains had antibiotic resistance, tolerance to artificial gastric juice and phenol (0.4%), enzymatic profile, and antagonistic activity against enteric pathogens (Enterobacter sakazakii, Salmonella typhimurium, Shigella flexneri 2a, Listeria monocytogenes, Yersinia enterocolitica and Aeromonas hydrophila). All strains survived well in artificial gastric juice at low pH (3.0) values for 4 h, possessed bile salt hydrolase activity and were susceptible to most antibiotics including vancomycin. Additionally, the isolates exhibited high tolerance to phenol, high cell surface hydrophobicity (>60%) and induced proliferation of murine splenocytes. All the four strains of present study suppressed the Con A-stimulated proliferation of the mouse spleen cells, although L. casei had the strongest suppressive effect. The results of this study suggest a potential application of the strains (following human clinical trials), for developing probiotic foods.
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Probiotic bacteria do not exhibit harmful biochemical properties such as β-glucosidase, α-chymotrypsin, β-glucuronidase, and N-acetyl-β-glucosaminidase activities, which are associated with intestinal diseases and can promote carcinogens and tumor promoters.
“While displaying probiotic properties, the bacteria do not exhibit harmful biochemical properties for the host organism, such as β-glucosidase, α-chymotrypsin, β-glucuronidase, and N-acetyl-β-glucosaminidase activities, which are associated with intestinal diseases and can promote the formation of carcinogens and tumor promoters [105].”
From Current Concepts in Probiotic Safety and Efficacy (2026) · cites this paper as [105]
The regulation of T-cell-mediated immune responses has also been observed in potential probiotic LAB.
“The regulation of T-cell-mediated immune responses has also been observed in the potential probiotic LAB [37,65].”
From Bioprophylactic potential of novel human colostrum probiotics via apoptotic induction of colon cancer cells and cell immune activation (2022) · cites this paper as [37]
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References 43 · Cited by 63
- Authors
- Kumar, M., Ghosh, M., & Ganguli, A.
- Affiliations
- Thapar Institute of Engineering & Technology (IN)
- Corresponding author
- Ganguli, Abhijit
- Venue
- World Journal of Microbiology and Biotechnology
- Published
- 2011-09-14
- Volume
- 28
- Issue
- 2
- Pages
- 703-711
- ISSN
- 0959-3993 (ISSN-L)
- 1573-0972
- OpenAlex
- W2014822901
- Cited by
- 63
- References
- 43
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2023: 5 · 2022: 6 · 2021: 4 · 2020: 5 · 2019: 6 · 2018: 1 · 2017: 7 · 2016: 2 · 2015: 8 · 2014: 4 · 2013: 3 · 2012: 6
- FWCI
- 6.07
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- World Journal of Microbiology and Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
- Keywords
- lactic acid bacteria, probiotics, pickled vegetables, fermented beverages, fermented rice, Madhuca longifolia, Lactobacillus helveticus, Lactobacillus casei, Lactobacillus delbrueckii, Lactobacillus bulgaricus, bile tolerance, gastric juice tolerance, phenol tolerance, bile salt hydrolase, antibiotic susceptibility, cell surface hydrophobicity, mitogen response, splenocyte proliferation
- MeSH terms
- Animals, Beverages, Cell Line, Fermentation, Humans, Lactobacillus, Vegetables, Probiotics, Cell Proliferation, Mice
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