HSV-1 stimulation-related protein HSRG1 inhibits viral gene transcriptional elongation by interacting with Cyclin T2
Open accessAbstract
The protein encoded by HSRG1 (HSV-1 stimulation-related gene 1) is a virally induced protein expressed in HSV-1-infected cells. We have already reported that HSRG1 is capable of interacting with transcriptional regulator proteins. To further analyze the effects of HSRG1 on the regulation of viral gene transcription, we expressed the HSRG1 protein in transfected cells and found that it postpones the proliferation of HSV-1. CAT (chloramphenicol acetyltransferase) assays also revealed that HSRG1 reduces transcription from HSV-1 promoters. Yeast two-hybrid and immunoprecipitation assays indicated that HSRG1 interacts with Cyclin T2, the regulatory subunit of P-TEFb, which is required for transcription elongation by RNA Pol II (RNAP II), and that amino acid residues 1-420 in Cyclin T2 are important for binding with HSRG1. Fluorescence assays suggested that the cellular localizations of those two proteins are influenced by their interaction. Further analyses with CAT assays revealed that HSRG1 inhibits the transcriptional activation by Cyclin T2 of viral promoters. Our results suggested that the inhibitory effects of HSRG1 on viral replication and proliferation are probably induced by its binding to Cyclin T2. Therefore, it is likely that HSRG1 inhibits viral gene transcriptional elongation by interacting with Cyclin T2.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
CCNT2 has been shown to be used by herpes simplex virus 1 (HSV-1) when it transcribes its genome.
“CCNT2 has also been shown to be used by herpes simplex virus 1 (HSV-1) when it transcribes its genome [46].”
From Multi-trait association studies discover pleiotropic loci between Alzheimer’s disease and cardiometabolic traits (2021) · cites this paper as [46]
Section 2.5 reports that Cyclin T2 increased transcriptional activity from HSV-1 promoters in transfected reporter assays, supporting a role in viral gene transcription. The paper infers a role during infection but does not directly show HSV-1 using Cyclin T2 to transcribe its genome. (LLM, conf=0.83)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Control of RNA Polymerase II Elongation Potential by a Novel Carboxyl-terminal Domain Kinase
Marshall, N. F., Peng, J., Xie, Z., & Price, D. H. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.43.27176 · Cited by 662 · see citing works
Flagged first, then by citations
Research works printed but not linked: 13
- Weir J P. Regulation of herpes simplex virus gene expression. Gene, 2001, 271: 117-130
- Shimomura Y. Herpes simplex virus latency, reactivation, and a new antiviral therapy for herpetic keratitis. Nippon Ganka Gakkai Zasshi, 2008, 112: 247-264
- Herbst-Kralovetz M, Pyles R. Toll-like receptors, innate immunity and HSV pathogenesis. Herpes, 2006, 13: 37-41
- Li J F, Liu L D, Ma S H, et al. HTRP--an immediate-early gene product induced by HSV1 infection in human embryo fibroblasts, is involved in cellular co-repressors. J Biochem, 2004, 136: 169-176
- Li Q H. Cytokines and Immediate Early Genes Reactions. Kunming: Yunnan University Press, 2001. 34-57
- Dong S, Dong C, Liu L, et al. Identification of a novel human sand family protein in human fibroblasts induced by herpes simplex virus 1 binding. Acta Virol, 2003, 47: 27-32
- Cottage A, Edwards Y J, Elgar G, et al. A new protein family: From nucleic acid to protein structure and function prediction. Comp Funct Genomics, 2001, 2: 226-235
- Poteryaev D, Spang A. A role of SAND-family proteins in endocyto- sis. Biochem Soc Trans, 2005, 33: 606-608
- Guo H X, Cun W, Liu L D, et al. Protein encoded by HSV-1 stimula- tion-related gene 1 (HSRG1) interacts with and inhibits SV40 large T antigen. Cell Prolif, 2006, 39: 507-518
- Marshall N F, Price D H. Purification of P-TEFb, a transcription fac- tor required for the transition into productive elongation. J Biol Chem, 1995, 270: 12335-12338
- Yu X, Li W Z, Liu L D, et al. Functional analysis of transcriptional regulation of herpes simplex virus type 1 tegument protein VP22. Sci China Ser C-Life Sci, 2008, 51: 966-972
- Peng J, Zhu Y, Milton J T, et al. Identification of multiple cyclin sub- units of human P-TEFb. Genes Dev, 1998, 12: 755-762
- Kurosu T, Zhang F, Peterlin B M. Transcriptional activity and sub- strate recognition of cyclin T2 from P-TEFb. Gene, 2005, 343: 173-179
Cited by
Sorted by citations
Program, T. V. M. V., Bone, W. P., Siewert, K. M., Jha, A., Klarin, D., Damrauer, S. M., Chang, K.-M., Tsao, P. S., Assimes, T. L., Ritchie, M. D., et al. (2021) · Alzheimer's Research & Therapy · doi:10.1186/s13195-021-00773-z · Cited by 41 · see citing works
- Authors
- Wu, W., Yu, X., Li, W., Guo, L., Liu, L., Wang, L., & Li, Q.
- Affiliations
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Corresponding author
- Li, QiHan
- Venue
- Science China Life Sciences
- Published
- 2011-04
- Volume
- 54
- Issue
- 4
- Pages
- 359-365
- ISSN
- 1674-7305 (ISSN-L)
- 1869-1889
- OpenAlex
- W1980978677
- Cited by
- 5
- References
- 1
- Citations by year
- 2021: 2 · 2013: 2 · 2012: 1
- FWCI
- 0.72
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Science China Life Sciences (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (30670094)
- Ministry of Education of the People's Republic of China
- Topics
- Health Sciences › Medicine › Epidemiology › Herpesvirus Infections and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Health Sciences › Medicine › Epidemiology › Cytomegalovirus and herpesvirus research
- Keywords
- herpes simplex virus type 1, P-TEFb, transcription elongation, viral transcription, RNA polymerase II, protein-protein interaction, viral replication inhibition, herpesvirus
- MeSH terms
- Animals, Chlorocebus aethiops, Cricetulus, Genes, Viral, Cricetinae, Humans, Promoter Regions, Genetic, Proteins, Transcription, Genetic, Vero Cells, Virus Replication, Gene Expression Regulation, Viral, CHO Cells, Herpesvirus 1, Human, Two-Hybrid System Techniques, Intracellular Signaling Peptides and Proteins, Cyclin T
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
