Translocator protein 18 kDa (TSPO) expression in multiple sclerosis patients
Open accessAbstract
Translocator protein (18 kDa) (TSPO) is a marker of inflammation in the brain. Positron emission tomography (PET) scans with ligands for this receptor show increased expression of TSPO in many neuropathologic conditions. However, expression of TSPO in the periphery and its possible correlation to central nervous system (CNS) inflammation has been largely unstudied. In this paper PBR28, a recently synthesized ligand for TSPO that is shown to have 80-fold higher specific binding than its predecessor PK11195, is used to quantify peripheral TSPO. Data presented in this study show that monocytes account for the majority of TSPO measured in peripheral blood mononuclear cells (PBMC), and that TSPO expression is stable over time in healthy individuals. Previous studies show that areas of increased PBR28 binding in the brains of multiple sclerosis (MS) patients correlate with active demylinating lesions found during magnetic resonance imaging (MRI). To measure peripheral TSPO expression in an inflammatory disease of the CNS, PBR28 is used in an in vitro radioligand binding assay to measure the amount of TSPO in the PBMC of MS and healthy donor cohorts. Surprisingly, MS patients are found to have a significantly lower amount of peripheral TSPO than healthy donors. We suggest that TSPO protein expression is a potential peripheral biomarker of MS, more research is needed to determine if peripheral TSPO expression may also be altered in other neuroinflammatory conditions.
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Our study did not find a strong expression of TSPO in T cells in the brain.
“A previous study has highlighted TSPO expression in several T-cell populations in blood (Harberts et al., 2013), but our study did not find a strong expression of TSPO in T cells in the brain.”
From A quantitative neuropathological assessment of translocator protein expression in multiple sclerosis (2019) · cites this paper as Harberts et al., 2013
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A quantitative neuropathological assessment of translocator protein expression in multiple sclerosis
Nutma, E., Stephenson, J. A., Gorter, R. P., de Bruin, J., Boucherie, D. M., Donat, C. K., Breur, M., van der Valk, P., Matthews, P. M., Owen, D. R., et al. (2019) · Brain · doi:10.1093/brain/awz287 · Cited by 138 · see citing works
“A previous study has highlighted TSPO expression in several T-cell populations in blood (Harberts et al. , 2013), but our study did not find a strong expression of TSPO in T cells in the brain.”
Cell-type-specific epigenetic effects of early life stress on the brain
Rahman, M. F., & McGowan, P. O. (2022) · Translational Psychiatry · doi:10.1038/s41398-022-02076-9 · Cited by 75 · see citing works
- Authors
- Harberts, E., Datta, D., Chen, S., Wohler, J. E., Oh, U., & Jacobson, S.
- Affiliations
- National Institutes of Health (US)
- Corresponding author
- Jacobson, Steven
- Venue
- Journal of Neuroimmune Pharmacology
- Published
- 2012-09-06
- Volume
- 8
- Issue
- 1
- Pages
- 51-57
- ISSN
- 1557-1890 (ISSN-L)
- 1557-1904
- PMC
- PMC4765953
- OpenAlex
- W1967030806
- Cited by
- 36
- References
- 1
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 3 · 2023: 3 · 2022: 2 · 2021: 3 · 2020: 1 · 2019: 3 · 2018: 2 · 2017: 3 · 2016: 5 · 2015: 3 · 2014: 4 · 2013: 1
- FWCI
- 1.85
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Journal of Neuroimmune Pharmacology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (UL1TR000058, KL2TR000057)
- National Institute of Neurological Disorders and Stroke (Z01NS002817, Z01NS003040)
- National Center for Advancing Translational Sciences
- Topics
- Health Sciences › Medicine › Pathology and Forensic Medicine › Multiple Sclerosis Research Studies
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pharmacological Receptor Mechanisms and Effects
- Keywords
- translocator protein (TSPO), multiple sclerosis, peripheral blood mononuclear cells, monocytes, peripheral biomarkers, neuroinflammation, radioligand binding assay, TSPO PET imaging
- MeSH terms
- Acetamides, Adult, Brain, Cell Separation, Female, Humans, Isoquinolines, Leukocytes, Mononuclear, Magnetic Resonance Imaging, Male, Middle Aged, Mitochondria, Multiple Sclerosis, Protein Binding, Pyridines, Radioligand Assay, Biomarkers, Receptors, GABA, Lipopolysaccharide Receptors, Radiopharmaceuticals, Positron-Emission Tomography, Membrane Potential, Mitochondrial, Real-Time Polymerase Chain Reaction
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