Best practices for the use and evaluation of animal serum as a component of cell culture medium
Abstract
Animal serum is a common additive for cell culture medium and is often required at 5 to 10% (v/v) for the attachment and growth of primary and continuous anchorage-dependent (monolayer) cultures. The use of animal serum in cell culture medium confers several advantages and also some risks. This article discusses the use of animal serum as a component of cell culture medium. The best practices associated with the sourcing, storage, thawing, testing, and mitigation of risk associated with the use of animal sera are among the topics described in this article.
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Animal sera present several limitations, mainly associated with their complexity: lability, viral contamination (potentially with multiple viruses), and batch-to-batch variability.
“Animal sera present several limitations, mainly associated with their complexity: lability, viral contamination (potentially with multiple viruses), and batch-to-batch variability (Brunner et al., 2010; Gstraunthaler, 2003; Nims and Harbell, 2017).”
From Guidance document on Good cell and tissue culture practice 2.0 (GCCP 2.0) (2021) · cites this paper as Nims and Harbell, 2017
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Drexler, H. G., & Uphoff, C. C. (2002) · Cytotechnology · doi:10.1023/a:1022913015916 · Cited by 340 · see citing works
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Guidance document on Good cell and tissue culture practice 2.0 (GCCP 2.0)
Pamies, D. (2021) · ALTEX · doi:10.14573/altex.2111011 · Cited by 100 · see citing works
- Authors
- Nims, R. W., & Harbell, J. W.
- Venue
- In Vitro Cellular & Developmental Biology - Animal
- Published
- 2017-07-21
- Volume
- 53
- Issue
- 8
- Pages
- 682-690
- ISSN
- 1071-2690
- 1543-706X
- Cited by
- 17
- References
- 1
- Language
- en
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