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Cited by 80

The DOI matches its Crossref record; 67 of the 67 references it cites were found in a registry or an index.

Best practices for cryopreserving, thawing, recovering, and assessing cells

Baust, John M., Campbell, Lia H., Harbell, John W.·2017·In Vitro Cellular & Developmental Biology - Animal
DOI 10.1007/s11626-017-0201-y
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Abstract

Long-term storage of cell stocks insures that cells are available for use whenever needed. Cryopreservation of cells is the method of choice for preservation of important or rare cell stocks. There are several factors to consider when establishing a protocol for freezing, thawing, and recovery of cells after storage. These parameters may include cell concentration, cryoprotectant choice and concentration, and thawing rate among others. Further, the assessment of cell viability and/or function prior to and following cryopreservation is imperative in order to accurately determine downstream utility as well for optimizing the cryopreservation process. This chapter is designed to provide guidance and insight into developing robust and successful protocols for preserving cells that will preserve cell stocks and provide optimal cell yield and viability.

Overview

Integrity signals

Registry

Crossref · 10.1007/s11626-017-0201-y

Cites

67 of 67 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

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Citations

Cites

Show all 67 references

Research works printed but not linked: 5

  1. Baust JM, Snyder KK, Mathew AJ, Van Buskirk RG, Baust JG (2006) Steps to improving preservation outcome: understanding key factors influencing efficacy. Bio/Technology 31(11):18–20
  2. Baust JM, Van Buskirk RG, Baust JG (2000) Cell viability improves following inhibition of cryopreservation-induced apoptosis. In Vitro Cell Dev Biol 36(4):262–270 · doi:10.1290/1071-2690(2000)036
  3. Coger R, Toner M (1995) Preservation techniques for biomaterials In: Biomedical Engineering Handbook. CRC Press, Boca Raton, pp 1557–1566
  4. Stylianou J, Vowels M, Hadfield K (2005) CryoStor significantly improves cryopreservation of haematopoetic stem cells (HSC). Cryotherapy 7(Suppl 1):117
  5. Van Buskirk RG, Baust JG, Baust JM (2006) Navigating the post-preservation viability fog. Bioprocess Tutorial Genet Eng News 26(19):38–38

Cited by

See all 80

Metadata

Authors
Baust, J. M., Campbell, L. H., & Harbell, J. W.
Venue
In Vitro Cellular & Developmental Biology - Animal
Publisher
Springer Science and Business Media LLC
Published
2017-11-02
Volume
53
Issue
10
Pages
855-871
ISSN
  • 1071-2690
  • 1543-706X
DOI
10.1007/s11626-017-0201-y
Cited by
80
References
67
Language
en
Licence
www.springer.com/tdm (unspecified)

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CoChat. (2026). Citation check: Best practices for cryopreserving, thawing, recovering, and assessing cells. https://app.cochat.ai/papers/10.1007/s11626-017-0201-y
The paper
Baust, J. M., Campbell, L. H., & Harbell, J. W. (2017). Best practices for cryopreserving, thawing, recovering, and assessing cells. In Vitro Cellular & Developmental Biology - Animal. https://doi.org/10.1007/s11626-017-0201-y

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.