Assessment of Genetic Stability and Instability of Tissue Culture-Propagated Plantlets of Aloe vera L. by RAPD and ISSR Markers
Abstract
Efficient plantlet regeneration with and without intermediate callus phase was achieved for a selected genotype of Aloe vera L. which is sweet in test and used as a vegetable and source of food. Random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) marker assays were employed to evaluate genetic stability of plantlets and validate the most reliable method for true-to-type propagation of sweet aloe, among two regeneration systems developed so far. Despite phenotypic similarities in plantlets produced through both regeneration systems, the differences in genomic constituents of plantlets produced through intermediate callus phase using soft base of inflorescence have been effectively distinguished by RAPD and ISSR markers. No polymorphism was observed in regenerants produced following direct regeneration of axillary buds, whereas 80% and 73.3% of polymorphism were observed in RAPD and ISSR, respectively, in the regenerants produced indirectly from base of the inflorescence axis via an intermediate callus phase. Overall, 86.6% of variations were observed in the plantlets produced via an intermediate callus phase. The occurrence of genetic polymorphism is associated with choice of explants and method used for plantlet regeneration. This confirms that clonal propagation of sweet aloe using axillary shoot buds can be used for commercial exploitation of the selected genotype where a high degree of fidelity is an essential prerequisite. On the other hand, a…
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In Aloe vera, direct regeneration from axillary buds showed 0% polymorphism, while indirect regeneration via callus from inflorescence base exhibited 80% polymorphism.
“axillary buds showed 0% polymorphism (true to type), inflorescence base exhibited via callus from the while indirect regeneration”
From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [84]
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- Authors
- Rathore, M. S., Chikara, J., Mastan, S. G., Rahman, H., Anand, K. G. V., & Shekhawat, N. S.
- Affiliations
- Central Salt and Marine Chemicals Research Institute (IN)
- Jai Narain Vyas University (IN)
- Corresponding author
- Rathore, Mangal Singh
- Venue
- Applied Biochemistry and Biotechnology
- Published
- 2011-09-02
- Volume
- 165
- Issue
- 5-6
- Pages
- 1356-1365
- ISSN
- 0273-2289 (ISSN-L)
- 1559-0291
- OpenAlex
- W2066114275
- Cited by
- 61
- References
- 27
- Citations by year
- 2025: 4 · 2024: 2 · 2023: 2 · 2022: 4 · 2021: 8 · 2020: 5 · 2019: 3 · 2018: 4 · 2017: 3 · 2016: 6 · 2015: 5 · 2014: 7 · 2013: 6 · 2012: 2
- FWCI
- 1.95
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Applied Biochemistry and Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Council of Scientific and Industrial Research, India
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
- Keywords
- Aloe vera, genetic stability, somaclonal variation, plantlet regeneration, axillary bud explants, RAPD markers, ISSR markers, genetic polymorphism, clonal propagation
- MeSH terms
- Aloe, Cells, Cultured, Genetic Markers, Phylogeny, Polymorphism, Genetic, Microsatellite Repeats, Random Amplified Polymorphic DNA Technique, Tissue Culture Techniques
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