Potential probiotic lactic acid bacteria of human origin induce antiproliferation of colon cancer cells via synergic actions in adhesion to cancer cells and short-chain fatty acid bioproduction
Abstract
The activities and modes of probiotic action of lactic acid bacteria isolated from infant feces were investigated for alternative application in the prevention and biotherapy of colon cancer. From a total of 81 isolates of Gram-positive rod and cocci bacteria obtained from healthy infants, only 15 isolates had the probiotic criteria which included growth inhibition against eight food-borne pathogens, no blood hemolysis, and tolerance to gastrointestinal tract properties such as pH 2.5 and 0.3 % bile salt. Four probiotic bacteria showed antiproliferation of colon cancer cells with the use of MTT and Trypan blue exclusion assay at the rates of 17-35 %. Through comparison of probiotic 16S rRNA sequences, they were identified as Pediococcus pentosaceus FP3, Lactobacillus salivarius FP25, L. salivarius FP35, and Enterococcus faecium FP51. Finding the mechanism of proliferative inhibition of colon cancer cells in this study indicated synergic induction by probiotic bacteria directly adhered to these cancer cells and triggered the bioproduction of short-chain fatty acids, mainly butyric and propionic acids. This study suggested that the use of these probiotics may be suitable as an alternative bioprophylactic and biotherapeutic strategy for colon cancer.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Probiotics of S. salivarius CP163 and S. salivarius CP208 might play an important role in adherence to colon cancer cells as the initial appearance of probiotic action against colon cancer.
“In agreement with the results of this study, human probiotic origins were found to adhere to colon cancer cells [12,44,54], indicating that probiotics of S. salivarius CP163 and S. salivarius CP208 might play an important role in adherence to colon cancer cells as the initial appearance of probiotic action against colon cancer.”
From Bioprophylactic potential of novel human colostrum probiotics via apoptotic induction of colon cancer cells and cell immune activation (2022) · cites this paper as [12]
The synergistic mechanism of probiotics against colon cancer in the adhesion to colon cancer cells and bioproduction of SCFAs has been postulated.
“The synergistic mechanism of probiotics against colon cancer in the adhesion to colon cancer cells and bioproduction of SCFAs has been postulated [12].”
From Bioprophylactic potential of novel human colostrum probiotics via apoptotic induction of colon cancer cells and cell immune activation (2022) · cites this paper as [12]
Probiotics of Pediococcus pentosaceus FP3, Lactobacillus salivarius FP25, L. salivarius FP35 and Enterococcus faecium FP51, which have a human origin, induced the inhibition of colon cancer cell proliferation.
“Similarly, it was found that probiotics of Pediococcus pentosaceus FP3, Lactobacillus salivarius FP25, L. salivarius FP35 and Enterococcus faecium FP51, which have a human origin, induced the inhibition of colon cancer cell proliferation [12].”
From Bioprophylactic potential of novel human colostrum probiotics via apoptotic induction of colon cancer cells and cell immune activation (2022) · cites this paper as [12]
The synergistic actions between adhesion to colon cancer cells as an initial process and later producing SCFA bioproducts might directly enhance the death of colon cancer cells.
“This might have resulted in the synergistic actions between adhesion to colon cancer cells as an initial process and later producing SCFA bioproducts, which could be found to directly enhance the death of colon cancer cells, similar to this study's previous hypothesis [12].”
From Bioprophylactic potential of novel human colostrum probiotics via apoptotic induction of colon cancer cells and cell immune activation (2022) · cites this paper as [12]
The citation lists haven’t been fetched yet
References 26 · Cited by 135
- Authors
- Thirabunyanon, M., & Hongwittayakorn, P.
- Affiliations
- Maejo University (TH)
- Corresponding author
- Thirabunyanon, Mongkol
- Venue
- Applied Biochemistry and Biotechnology
- Published
- 2012-12-13
- Volume
- 169
- Issue
- 2
- Pages
- 511-525
- ISSN
- 0273-2289 (ISSN-L)
- 1559-0291
- OpenAlex
- W2100816639
- Cited by
- 135
- References
- 26
- Citations by year
- 2026: 2 · 2025: 10 · 2024: 12 · 2023: 13 · 2022: 18 · 2021: 23 · 2020: 15 · 2019: 9 · 2018: 6 · 2017: 3 · 2016: 9 · 2015: 7 · 2014: 5 · 2013: 3
- FWCI
- 7.37
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Applied Biochemistry and Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Thailand Institute of Scientific and Technological Research
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Cancer Research and Treatments
- Keywords
- probiotics, lactic acid bacteria, colon cancer, antiproliferative activity, tumor cell adhesion, short-chain fatty acids, butyrate, propionic acid, Pediococcus pentosaceus, Lactobacillus salivarius, Enterococcus faecium, cancer prevention, bile salt tolerance
- MeSH terms
- Bacterial Adhesion, Fatty Acids, Volatile, Feasibility Studies, Humans, Infant, Newborn, Intestines, Lactobacillus, Neoplasms, Experimental, Caco-2 Cells, Probiotics, Cell Proliferation
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
