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Cited by 101Enzyme Catalysis and ImmobilizationEnzyme Production and Characterizationbiodegradable polymer synthesis and properties

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Fungal Hydrophobin RolA Enhanced PETase Hydrolysis of Polyethylene Terephthalate

Puspitasari, Nathania, Tsai, Shen-Long, Lee, Cheng-Kang·2021·Applied Biochemistry and Biotechnology
DOI 10.1007/s12010-020-03358-y
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Abstract

Polyethylene terephthalate (PET) becomes one of the most well-known polyesters and is widely used as packaging material. Recently, polyethylene terephthalate hydrolase (PETase) has emerged as a potential biocatalyst demonstrating the ability to degrade polyethylene terephthalate (PET). We showed that the rate of PETase hydrolysis could be significantly increased in the presence of hydrophobin RolA. Hydrophobins represent a class of small fungal protein that has a high surface-active substance and can spontaneously self-assemble at hydrophilic-hydrophobic interfaces. In this work, a class I hydrophobin named RolA was extracted from the mycelium pellet collected from a fermentation culture of Aspergillus oryzae. The SDS-PAGE analysis of the isolated RolA showed the presence of 11 kDa polypeptide. Recombinant PETase from Ideonella sakaiensis was also successfully expressed in Escherichia coli as a soluble protein with molecular weight approximately 30 kDa. The hydrophobin RolA could enhance the PET hydrolysis in the presence of the recombinant PETase. The hydrolysis of PET bottle by RolA-PETase achieved the highest weight loss of 26% in 4 days. It is speculated that the wetting effect of RolA acts on PET surface converts PET to become hydrophilic that leads PETase easier to contact and attack the surface. Graphical Abstract.

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Metadata

Authors
Puspitasari, N., Tsai, S.-L., & Lee, C.-K.
Affiliations
National Taiwan University of Science and Technology (TW)
Corresponding author
Lee, Cheng-Kang
ORCID
Venue
Applied Biochemistry and Biotechnology
Publisher
Springer Science and Business Media LLC
Published
2020-06-06
Volume
193
Issue
5
Pages
1284-1295
ISSN
  • 0273-2289 (ISSN-L)
  • 1559-0291
DOI
10.1007/s12010-020-03358-y
OpenAlex
W3033189558
Cited by
101
References
0
Citations by year
2026: 17 · 2025: 14 · 2024: 16 · 2023: 22 · 2022: 14 · 2021: 17 · 2020: 1
FWCI
4.33
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Applied Biochemistry and Biotechnology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
  • Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
Keywords
polyethylene terephthalate, PETase, class I hydrophobins, PET hydrolysis, Aspergillus oryzae, Ideonella sakaiensis, surface wetting, polyester biodegradation, recombinant protein expression
MeSH terms
Aspergillus oryzae, Electrophoresis, Polyacrylamide Gel, Escherichia coli, Fungal Proteins, Hydrolysis, Polyethylene Terephthalates, Hydrophobic and Hydrophilic Interactions

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